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Citations for New England Biolabs :
1 - 50 of 8993 citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Genomics 2020Quote: ... and SAP (NEB, M0371S) treatment ...
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bioRxiv - Systems Biology 2019Quote: ... and shrimp alkaline phosphatase (SAP, NEB). The concentrations of the caps ...
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bioRxiv - Genomics 2024Quote: ... followed by dephosphorylation with Quick SAP (NEB). gRNAs were re-folded prior ABE8e:gRNA complexation ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were treated with Shrimp Alkaline Phosphatase (SAP; from NEB). In short ...
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bioRxiv - Genomics 2019Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 µl at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was pre-treated with SAP (10 U, New England Biolabs #M0371S) in CutSmart buffer in a total volume of 10 μl at 37°C for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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bioRxiv - Neuroscience 2024Quote: ... a V5 tag was inserted after the start codon of Ten-m cDNA (isoform B) in the plasmid pUAST-attB-Ten-m5 using the Q5 site-directed mutagenesis kit (New England Biolabs). To generate the UAS-V5-Ten-m-ΔICD and UAS-V5-Ten-m-ΔECD constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1-ATP6V1H(Δ176-191)-FLAG and pcDNA3.1-ATP6V1H(Δ176-193)-FLAG were generated using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). pENTR-ATP6V1H-FLAG was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... the pTXB1 plasmid was double-digested with Nde-I and Sap-I (New England Biolabs, Ipswich, MA, USA) enzymes at the respective restriction sites to generate binding sites for insert ligation ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified products were cleaned using Exonuclease-Shrimp Alkaline Phosphatase (Exo-SAP) mixture (New England Biolabs, Ipswich, Massachusetts) and sequenced bidirectionally using BigDye v3.1 terminator kit in ABI 3500XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-LC3B-K51R and FLAG-BIRC6-C4597A were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0552). The pSuper-Birc6-shRNA plasmid was generated by cloning shRNA for rat Birc6 (5’-CACCCCAGTAGTCATACCA-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... Bleo-treated WT-PNKP-FLAG and K226R-PNKP-FLAG cells using 10 μg of custom-made rabbit polyclonal anti-AcK226 Abs (Ez Biolabs). All other subsequent steps and buffers used for IP were the same as described earlier (17,18) ...
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bioRxiv - Cell Biology 2021Quote: ... were subcloned into phCMV3 to express C-terminal FLAG-tagged CatSper subunits (phCMV3-CatSperd or z-Flag) using NEBuilder® HiFi DNA Assembly Kit (NEB). A stop codon was placed at the upstream of HA-encoding sequences of phCMV3 vector for FLAG-tagged CatSper subunit cloning.
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bioRxiv - Cell Biology 2023Quote: ... DmMIC10b(C64S)-FLAG were produced using the Site-Directed Mutagenesis Kit (NEB) and the oligonucleotides given below.
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were run by electrophoresis on a 2% agarose gel to ensure the absence of primer-dimers and PCR samples for each primer pair-opsin target were purified using Exonuclease (EXO) and Shrimp alkaline phosphatase (SAP) (NEB) prior to Sanger Sequencing to confirm gene-specific amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of DNA isolate was used for PCR (primers are in supplementary table 1.4) and PCR cleanup was performed using Exo-SAP (NEB, M0293, M0371). Sanger Sequencing reactions were prepared using (Thermo Fisher # 4337455) ...
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bioRxiv - Genetics 2022Quote: ... or FLAG (amino acid sequence: DYKDDDDK) with a kit (Gibson assembly kit from New England Biolabs, catalogue # E5510S). The different combinations were cloned into pJFRC7-20XUAS-IVS-mCD8::GFP (Addgene # 26220 ...
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bioRxiv - Plant Biology 2021Quote: ... 500 ng of the SAP-treated RNA was treated with 12.5 units RNA 5’ Pyrophophohydrolase (RppH; New England BioLabs; Ipswitch, MA, USA) in 1X T4 RNA Ligase Buffer (New England BioLabs ...
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bioRxiv - Cell Biology 2022Quote: ... and ligated into pcDNA5/FRT/TO-N-FLAG-BirA* using Quick Ligation Kit (NEB). Top10 competent E ...
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bioRxiv - Microbiology 2024Quote: PCR products of Asaia positive samples were cleaned up using Exonuclease I (Exo I) and Shrimp Alkaline Phosphate (Exo-SAP protocol) according to the protocol of New England Biolabs (NEB, MA, and USA) and sent for sequencing ...
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bioRxiv - Microbiology 2021Quote: The pCDNA3.1-hACE2-FLAG plasmid and a Q5 Site-Directed Mutagenesis Kit (New England Biolabs) were used according to the manufacturer’s specifications to generate recombinant plasmids that expressed hACE2 N-glycosylation mutants ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-Flag immobilized Mif2-6xHis-6xFlag was then treated with lambda-phosphatase (New England Biolabs) according to the manufacturer’s instruction and incubated for 2 h at 30 °C and 1200 rpm in a thermomixer ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-Flag immunoprecipitates were subject to Lambda phosphatase treatment (NEB #P0753L, Ipswich, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-βTrCP2 (WD40Mut) were generated using the Q5 site-directed mutagenesis kit (NEB; Cat. #E0552S). The βTrCP1 WD40Mut changes amino acid 510 from R>A based on isoform 1 in uniprot (R474A when referring to isoform 2 in uniprot) ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Microbiology 2023Quote: ... rLCMV-GP1-FLAG was generated by insertion PCR utilising Q5 site-directed mutagenesis kit (New England BioLabs) and FLAG primers ...
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bioRxiv - Developmental Biology 2020Quote: UASp-KibS677A-GFP-FLAG was generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Catalog #E0554S) using primers KibS677A (see primers table) ...
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bioRxiv - Cell Biology 2023Quote: ... pREP81-FLAG plasmid carrying wild-type srs2+ was constructed using the Gibson Assembly® Cloning Kit (NEB E5510S). After Gibson cloning ...
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bioRxiv - Biochemistry 2022Quote: ... the pcDNA3.1 Flag-mRBPJ A284V CRr and the pcDNA3.1 Flag-mRBPJ F261A/A284V CRr were digested with NotI (NEB) and the cDNAs were inserted into the pMY-Bio IRES Blasticidin pre-digested with NotI (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... σA-FLAG and RbpA-FLAG proteins were prepared by PCR using Q5® High-Fidelity DNA Polymerase (NEB) with primers #3130 + #3131 (HelD) ...
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bioRxiv - Developmental Biology 2022Quote: ... EGFP-FLAG was removed using BamHI (NEB) and NotI (NEB ...
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bioRxiv - Cell Biology 2022Quote: Recombinant C-terminally FLAG-Tagged TEX264 was produced by PURExpress in vitro protein synthesis kit (E6800; New England Biolabs). Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B ...
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bioRxiv - Cell Biology 2022Quote: ... ICL3 and S305A mutations were generated using back-to-back primers on pCDNA3.1-HCAR1-Flag vector by Q5 Site-Directed Mutagenesis Kit (NEB). Fluorogen activating peptide fusion to HCAR1 was synthesized with insertion of HCAR1 using BsmI site into pMFAP-β1 vector (Spectragenetics) ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
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bioRxiv - Molecular Biology 2023Quote: ... pLenti-PGK-ATP6V1H(Δ176-191)-FLAG-Puro was produced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). M4P-EGFP-LC3B and pOGP were kind gifts from F ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 5U Enterokinase (cat#: P8070S, NEB) in the 1x EK reaction buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 10U CIP Phosphatase (cat#: M0290S, NEB) in the 1x CIP buffer (50 mM Potassium Acetate ...
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bioRxiv - Microbiology 2019Quote: In vitro transcribed 3X-FLAG tagged manY transcripts – wild-type or Δenh (1 pmol) were translated in vitro using the PURExpress translation kit (NEB). Translation reactions were stopped by adding Laemmli sample buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... were generated as described previously.23 In vitro transcription/translation of (fMVF-Flag was carried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers OL87 ...
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bioRxiv - Cell Biology 2021Quote: ... A 12xHis tag was cloned into the N-terminus of pcDNA3.1-MICAL1-FLAG using Q5® Site-Directed Mutagenesis Kits (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
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bioRxiv - Microbiology 2023Quote: Epitope-tagged constructs using the GST tag or the 3X FLAG tag were generated using the HiFi DNA Assembly cloning kit (NEB). MntS was cloned into pGEX-6-1 and the GST-MntS fusion was subcloned into pKT25c such that the resulting plasmid lacked the T25 region ...