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Citations for New England Biolabs :
1 - 50 of 2110 citations for Adenovirus Type 5 Particles CMV Luciferase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Renilla luciferase was PCR amplified from pMT-DEST48-FLP 31 with Renilla Luciferase Forward: 5’-TGGAAGCTTGGCATTCCGGTACTGTTGGTAAAGCCACCATGACTTCGAAAGTTTATG-3’ and Renilla Luciferase Reverse: 5’-TGGAAGCTTTTATTATTGTTCATTTTTGAGAAC-3’ and digested with HindIII (NEB). Renilla luciferase was then ligated into pGL3-Control digested HindIII (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... the adenovirus expression constructs were linearized using PacI restriction enzyme (NEB) and transfected into HEK293A cells cultured in 6-well plates using Lipofectamine 2,000 to produce the initial adenovirus ...
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bioRxiv - Cell Biology 2020Quote: ... pCS2-luciferase CDS or pCS2-luciferase with G3BP1 5’UTR and 3’UTR were linearized with Sal I (New England Biolabs) and gel purified (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Biochemistry 2023Quote: ... to pcDNA4/TO and PiggyBac-CMV-MCS-IRES-mCherry and PiggyBac-CMV-MCS-IRES-mNeongreen by PCR using Q5 High Fidelity 2X mastermix (NEB). PiggyBac-CMV-MCS-IRES-NLS-TagBFP was used as empty vector control ...
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bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
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bioRxiv - Microbiology 2021Quote: ... Chitin magnetic particles (New England Biolabs, #E8036L) were washed in sterile artificial seawater (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Hydrogel chitin particles (New England Biolabs, #E8036L) were washed in sterile artificial seawater (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... pLenti CMV-GFP-Puro was digested with BamHI and SalI (NEB) to remove GFP cassette and served as the vector backbone ...
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bioRxiv - Cell Biology 2023Quote: ... we first cut pHAGE-CMV-EGFP-W1 with NotI (NEB # R0189S), followed by the treatment with DNA Polymerase I ...
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bioRxiv - Immunology 2021Quote: We quantified plasma luciferase activity by a BioLux Gaussia Luciferase Assay Kit (NEB, Ipswich, MA) from 10 μL plasma acquired by retro-orbital bleeding ...
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bioRxiv - Microbiology 2019Quote: ... Luciferase reporters were constructed using the luciferase reporter vector pBBRlux digested with BamHI and SpeI (NEB). Expression vectors for VpsT and VpsR were constructed by removing the ribosome binding site (RBS) ...
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bioRxiv - Neuroscience 2023Quote: ... downstream of the CMV promoter using NEBuilder HiFi Assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... 5’ and 3’ flanking sequences containing recognition sites for the Type II restriction enzyme BsaI-HF®v2 (NEB, R3733) were added to each IUPAC DNA block ...
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bioRxiv - Biophysics 2021Quote: ... The 5’-terminus was capped with a type I 7-methylguanosine cap (m7G) using the Vaccinia Capping System (NEB, M2080S) and 2’-O-methyltransferase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The insert is fused during cloning through an overlapping proline-encoding CCG codon introduced by reverse and forward primers at the 3’- and 5’-end of the sybodies and MBP respectively and released by digestion with the Type IIS restriction enzyme SapI (NEB). The second proline of the linker is encoded in the forward primer of the MBP (3’ of the overlapping CCG codon ...
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bioRxiv - Microbiology 2021Quote: ... reverse: 5’-GATGGCGTGGAACCATGTC-3’) were obtained from the wild type plasmids pCMV-hnCoV-S via Q5 SiteDirected Mutagenesis Kit (NEB). pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward ...
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bioRxiv - Microbiology 2022Quote: ... Gaussia princeps luciferase (New England Biolabs, #E8023) or Tubulin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Secreted cypridina Luciferase (cLuc) activity from the internal control plasmid was similarly measured using the cypridina Luciferase kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Type IIs RE (0.5 μl, NEB), vector backbone and inserts were combined to make 10 μl ...
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bioRxiv - Neuroscience 2020Quote: ... pGL3 BRE Luciferase was digested with KpnI (NEB), blunted using a commercial blunting kit (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-Gaussia luciferase (E8023, New England Biolabs) at a 1:2,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Gaussia luciferase (New England Biolabs, E8023, 1:10,000), and revealed with appropriate secondary antibodies (GE Healthcare or Sigma ...
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bioRxiv - Microbiology 2020Quote: ... the BioLux® Gaussia Luciferase Assay Kit (NEB, discontinued) or the GAR-2B Gaussia Luciferase Assay (Targeting Systems ...
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bioRxiv - Synthetic Biology 2020Quote: ... Wild type ϕ29 was purchased from NEB as positive control ...
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bioRxiv - Biophysics 2022Quote: ... type IIS enzymes (BsaI, New England Biolabs #R3733 ...
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bioRxiv - Cancer Biology 2024Quote: ... DNAse type IV (New England Biolabs/ Bioconcept) in DMEM/F12 (Sigma-Aldrich ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Systems Biology 2022Quote: ... JunDN-HA-T2A-GFP was amplified from the pLVX-CMV backbone using Phusion Mastermix (NEB, catalog # M0531), and inserted into the empty backbone of pAK_Tol2_TRE_Puro (Addgene ...
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bioRxiv - Cancer Biology 2021Quote: pLenti CMV Hygro DEST (w117-1) was digested with SalI and BamHI (New England BioLabs Inc., MA) according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... the CMV enhancer/promoter region was amplified from the pcDNA3.0 backbone using Phusion High-Fidelity Polymerase (NEB) and subcloned into the pUC19 vector upstream of the circEfnb2 or circMini backsplicing cassettes ...
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bioRxiv - Cell Biology 2020Quote: ... or BioLux® Cypridina Luciferase Assay Kit (New England Biolabs) and Gaussia luciferase was assayed from 1:40 diluted cell culture supernatant using either the Stop & Glo reagent from the DualLuciferase® Reporter Assay System (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... or BioLux® Gaussia Luciferase Assay Kit (New England Biolabs) in a Lumat LB 9507 luminometer (Berthold Technologies).
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bioRxiv - Microbiology 2021Quote: ... or a Biolux Gaussia luciferase assay kit (New England BioLabs) and a GloMax microplate reader (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... type IIS restriction enzyme SapI (New England Biolabs), which recognizes asymmetric DNA sequences and cleave outsides their 5’-GCTCTTCN↓NNN -3’ recognition site ...
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bioRxiv - Molecular Biology 2023Quote: Wild‐type λ‐DNA was purchased from NEB. For generation of 2x T7‐4xCBSs λ‐DNA ...
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bioRxiv - Genomics 2022Quote: ... PIP particles were suspended in buffer with 29 U/mL Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Molecular Biology 2022Quote: Bclx and CD44-v5 minigenes were cloned in EGFP-C1 plasmid following the CMV promoter using ExoIII (NEB) enzyme following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Renilla luciferase was then ligated into pGL3-Control digested HindIII (NEB) to make pGL3-Renilla-FF ...
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bioRxiv - Cell Biology 2019Quote: ... and assessed for luminescence using the stabilized Gaussia luciferase kit (NEB; 50 μl of a 1:1 dilution with 18 MΩ water ...
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bioRxiv - Cell Biology 2022Quote: MaMTH reporter cells stably expressing Gaussia princeps luciferase (New England Biolabs) under the control of a 5xGAL4 UAS were seeded in 96-well tissue culture-treated plates (∼15 000 cells/well ...
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bioRxiv - Cell Biology 2019Quote: The CMV-EPO-eGFP-p2A-A1AT-mCherry construct was assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequences derived from PCSK9-eGFP-p2A-A1AT-mCherry (32 ...
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bioRxiv - Physiology 2022Quote: ... The scAAV-CMV-GFP plasmid was digested with EcoRI and HpaI (New England Biolabs; Cat. Nos. R3101 and R0105) and the linearized scAAV-CMV plasmid without the GFP was gel purified ...
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bioRxiv - Synthetic Biology 2023Quote: ... To generate standard curves for absolute qPCR we linearized pTransgene (p8-pAAV2.1 CMV eGFP3)37 with XhoI and NheI enzymes (NEB) and diluted in PCR-grade water from 2.5× 10^8 to 25 copies/μL in 10-fold serial dilutions ...
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bioRxiv - Neuroscience 2023Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI and NHE-I (NEB #R3131) cut sites ...
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bioRxiv - Microbiology 2019Quote: ... Assays were performed using NEB BioLux Gaussia Luciferase (GLuc) kit (NEB #E3300L) following the stabilized protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: Modified pGL4 luciferase constructs were generated via Gibson cloning (New England Biolabs) to contain an 1826 bp oligo corresponding to region of interest in CECR5/HDHD5 with variants corresponding to a European reference (EUR-EUR) ...