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Citations for New England Biolabs :
1 - 50 of 2260 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... pLenti CMV-GFP-Puro was digested with BamHI and SalI (NEB) to remove GFP cassette and served as the vector backbone ...
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bioRxiv - Systems Biology 2022Quote: ... JunDN-HA-T2A-GFP was amplified from the pLVX-CMV backbone using Phusion Mastermix (NEB, catalog # M0531), and inserted into the empty backbone of pAK_Tol2_TRE_Puro (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... the adenovirus expression constructs were linearized using PacI restriction enzyme (NEB) and transfected into HEK293A cells cultured in 6-well plates using Lipofectamine 2,000 to produce the initial adenovirus ...
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bioRxiv - Physiology 2022Quote: ... The scAAV-CMV-GFP plasmid was digested with EcoRI and HpaI (New England Biolabs; Cat. Nos. R3101 and R0105) and the linearized scAAV-CMV plasmid without the GFP was gel purified ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Biochemistry 2023Quote: ... to pcDNA4/TO and PiggyBac-CMV-MCS-IRES-mCherry and PiggyBac-CMV-MCS-IRES-mNeongreen by PCR using Q5 High Fidelity 2X mastermix (NEB). PiggyBac-CMV-MCS-IRES-NLS-TagBFP was used as empty vector control ...
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bioRxiv - Microbiology 2022Quote: ... Briefly 5 µg pAc CrPV 5’UTR-1A-GFP-3’ or pCrPV-3 plasmids were linearized with Eco53KI (NEB) or 5 µg pTOPO dsRNA plasmids with EcoR1 (NEB ...
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bioRxiv - Microbiology 2021Quote: ... forward and reverse primer for GFP (forward 5’TCGACAGTCAGCCGCATCT3’ and reverse primer 5’CCGTTGACTCCGACCTTCA3’) respectively using 1μl taq polymerase (NEB, USA). The PCR amplification was performed as per the following cycle ...
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bioRxiv - Microbiology 2021Quote: ... Chitin magnetic particles (New England Biolabs, #E8036L) were washed in sterile artificial seawater (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Hydrogel chitin particles (New England Biolabs, #E8036L) were washed in sterile artificial seawater (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... CrPV 5’UTR-1A-GFP-3’ UTR was generated using Gibson assembly (NEB Gibson assembly). The respective mutants were generated using Site directed mutagenesis ...
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bioRxiv - Cell Biology 2023Quote: ... we first cut pHAGE-CMV-EGFP-W1 with NotI (NEB # R0189S), followed by the treatment with DNA Polymerase I ...
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bioRxiv - Neuroscience 2023Quote: ... downstream of the CMV promoter using NEBuilder HiFi Assembly (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: ... 5’ and 3’ flanking sequences containing recognition sites for the Type II restriction enzyme BsaI-HF®v2 (NEB, R3733) were added to each IUPAC DNA block ...
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bioRxiv - Biophysics 2021Quote: ... The 5’-terminus was capped with a type I 7-methylguanosine cap (m7G) using the Vaccinia Capping System (NEB, M2080S) and 2’-O-methyltransferase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The insert is fused during cloning through an overlapping proline-encoding CCG codon introduced by reverse and forward primers at the 3’- and 5’-end of the sybodies and MBP respectively and released by digestion with the Type IIS restriction enzyme SapI (NEB). The second proline of the linker is encoded in the forward primer of the MBP (3’ of the overlapping CCG codon ...
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bioRxiv - Microbiology 2021Quote: ... reverse: 5’-GATGGCGTGGAACCATGTC-3’) were obtained from the wild type plasmids pCMV-hnCoV-S via Q5 SiteDirected Mutagenesis Kit (NEB). pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward ...
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bioRxiv - Synthetic Biology 2021Quote: ... Type IIs RE (0.5 μl, NEB), vector backbone and inserts were combined to make 10 μl ...
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bioRxiv - Neuroscience 2020Quote: ... + 5 % non-fat milk for 1 hour at room temperature before incubating with primary antibody: anti-GFP (1:1000, Biolabs) or anti-actin (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Base editing sgRNA directed against the SF3B1 K700 locus was designed using BE-Designer28 and cloned into pLKO.5 Puro-2A-GFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each of the synthesized DNA fragments was amplified by PCR and inserted at the 5’ of the GFP gene in the pPha-NR vector (NovoPro Bioscience) using NEBuilder HiFi DNA Assembly (New England BioLabs). Five μg of the pPha-NR constructs were introduced into P ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3.3 kb DNA sequence upstream of each respective gene was amplified using PCR and cloned into the vector containing the sequence gfp::rab-7::rab-7 3’UTR amplified from plasmid rgef-1p::gfp::rab-7::rab-7 3’UTR using PCR and primers 5’-atgagtaaaggagaagaacttttca-3’ and 3’-aagcttatcgataccgtcgac-5’ were used to generate tissue-specific gfp::rab-7::rab-7 3’UTR by using Gibson assembly (NEB E2611).
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bioRxiv - Developmental Biology 2023Quote: ... the tbb-2 3’UTR sequence was amplified from genomic DNA using PCR and primers 5’-tggatgaactatacaaatagatgcaagatcctttcaagca-3’ and 3’-aggttttcaccgtcatcacccgcgaaaaacccatgtaagt-5’ and the vector containing the sequence of pie-1p::his-15::gfp amplified from plasmid containing pie-1p::his-15::gfp::egg-6 3’UTR using PCR and primers 5’-ggtgatgacggtgaaaacct-3’ and 3’-ctatttgtatagttcatccatgcc-5’ were used to generate pie-1p::his-15::gfp::tbb-2 3’UTR by using Gibson assembly (NEB E2611). To add the wild type or mutant 3xmir-51 seed sequences ...
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bioRxiv - Developmental Biology 2023Quote: ... rgef-1p and gfp were inserted into a vector containing the sequence rab-7 amplified using PCR and primers 5’-atgtcgggaaccagaaagaa-3’ and 3’-aagcttatcgataccgtcgac-5’ to create rgef-1p::gfp::rab-7::rab-7 3’UTR using Gibson assembly (NEB E2611). A full list of reagents and resources can be found in table S2.
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bioRxiv - Synthetic Biology 2020Quote: ... Wild type ϕ29 was purchased from NEB as positive control ...
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bioRxiv - Biophysics 2022Quote: ... type IIS enzymes (BsaI, New England Biolabs #R3733 ...
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bioRxiv - Cancer Biology 2024Quote: ... DNAse type IV (New England Biolabs/ Bioconcept) in DMEM/F12 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: pLenti CMV Hygro DEST (w117-1) was digested with SalI and BamHI (New England BioLabs Inc., MA) according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... the CMV enhancer/promoter region was amplified from the pcDNA3.0 backbone using Phusion High-Fidelity Polymerase (NEB) and subcloned into the pUC19 vector upstream of the circEfnb2 or circMini backsplicing cassettes ...
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bioRxiv - Microbiology 2020Quote: ... type IIS restriction enzyme SapI (New England Biolabs), which recognizes asymmetric DNA sequences and cleave outsides their 5’-GCTCTTCN↓NNN -3’ recognition site ...
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bioRxiv - Molecular Biology 2023Quote: Wild‐type λ‐DNA was purchased from NEB. For generation of 2x T7‐4xCBSs λ‐DNA ...
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bioRxiv - Genomics 2022Quote: ... PIP particles were suspended in buffer with 29 U/mL Proteinase K (NEB #P8107S) and 70 mM DTT (Sigma #D9779 ...
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bioRxiv - Molecular Biology 2022Quote: Bclx and CD44-v5 minigenes were cloned in EGFP-C1 plasmid following the CMV promoter using ExoIII (NEB) enzyme following manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the GFP-KLP-18ts and GFP-ΔC-stalk were created via site-directed mutagenesis (NEB) from this GFP-stalk construct ...
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bioRxiv - Developmental Biology 2020Quote: ... GFP-MyoXΔPH and GFP-MyoXΔFERM were generated with Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S), where the amino acids (1212-1253 ...
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bioRxiv - Cell Biology 2021Quote: All constructs (GFP-EXO70B2, EXO70B2-GFP and mRuby2-EXO70B2) were prepared with the Phusion PC (NEB, USA) reaction using as a template either genomic DNA for intron-less genes and promoters or cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... and iLID constructs were generated by PCR amplifying the parent constructs in pcDNA3.1 (GFP-Gγ3, GFP-Gγ9, and Venus-iLID) with overhangs containing expected nucleotide mutations and DpnI (NEB) digestion (to remove the parent construct ...
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bioRxiv - Cell Biology 2019Quote: The CMV-EPO-eGFP-p2A-A1AT-mCherry construct was assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequences derived from PCSK9-eGFP-p2A-A1AT-mCherry (32 ...
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bioRxiv - Synthetic Biology 2023Quote: ... To generate standard curves for absolute qPCR we linearized pTransgene (p8-pAAV2.1 CMV eGFP3)37 with XhoI and NheI enzymes (NEB) and diluted in PCR-grade water from 2.5× 10^8 to 25 copies/μL in 10-fold serial dilutions ...
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bioRxiv - Neuroscience 2023Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI and NHE-I (NEB #R3131) cut sites ...
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bioRxiv - Cell Biology 2020Quote: ... and pFLOE1p:FLOE1ΔDUF-GFP were obtained by modifying pFLOE1p:FLOE1-GFP using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priDSdeletion-FWD/REV ...
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bioRxiv - Neuroscience 2023Quote: ... We prepared cDNA libraries from GFP+ and GFP-samples (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB) from RNA enriched with mRNA through bead-based polyA selection ...
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bioRxiv - Synthetic Biology 2020Quote: ... Type IIS assembly with BsmbI (New England Biolabs, MA, USA) and annealed oligo cloning ...
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bioRxiv - Immunology 2021Quote: VH and VL sequences of candidate sequences were cloned into a pcDNA.3 based vector with dual CMV promotor harboring IgG1 heavy chain and light chain backbone using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... and the CMV promoter was removed to prevent aberrant transcription by digesting the plasmid with ClaI-HF and BamHI-HF (NEB), gel extracting ...
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bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the Ef1a promoter with a CMV promoter cassette in pLV-Ef1a-Cre-U6-sgRNA(MS2) using PCR and HiFi assembly (NEB). The pLV-CMV-CreERT2-P2A-[puroR/zeoR/hygR]-U6-sgRNA(MS2 ...
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced into either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... The SETD3 V266D mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 V266D construct was then used as a template to sequentially incorporate Q257A and Y288P mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Biophysics 2022Quote: ... This SETD3 mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs).