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Citations for New England Biolabs :
1 - 50 of 2199 citations for 6 Amino 5 formamido uracil since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Uracil-DNA glycosylase (NEB, #M0280S, 5 U/ul) was added and the reaction was incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The labeled oligonucleotide (∼5 pmol) was treated with uracil glycosylase (NEB) in 1x UDG buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S ...
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bioRxiv - Biochemistry 2024Quote: ... Uracil was removed by uracil DNA glycosylase (0.025 U/μl, New England Biolabs) at 37 °C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins, UK) and 0.75U uracil-DNA glycosylase (NEB) were added to 10µg protein lysate at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 U of uracil DNA glycosylase (NEB), 100 μM RSH 5194 and 1x UDG buffer (NEB)48 ...
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bioRxiv - Immunology 2020Quote: ... Residual template switch adaptor was removed by incubation with 5 U of uracil DNA glycosylase (New England BioLabs) for 40 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... Looped adapter sequences were opened by removal of uracil from hairpin structures by adding 3 units of USER enzyme (Uracil-Specific Excision Reagent) (NEB, M5505S) and incubation at 37 °C for 15 min ...
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bioRxiv - Immunology 2020Quote: ... cDNA synthesised with SMARTNNN oligos were treated with 1 μl of Uracil DNA glycosylase (5 U/μl, New England Biolabs) and incubated for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5 µL of uracil DNA glycosylase enzyme (NEB M0280S), 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 units of uracil DNA glycosylase (New England BioLabs), and 10 mM EDTA] ...
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bioRxiv - Biochemistry 2022Quote: ... The annealed duplex was treated with uracil glycosylase (NEB) in 1×UDG buffer for 2 h at 37°C followed by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of 10x uracil DNA glycosylase buffer (NEB M0280S), 2.5 µL RNase A (M0280S ...
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bioRxiv - Developmental Biology 2020Quote: ... and the Uracil-Specific Excision Reagent enzyme (NEB, cat.: M5505L) was used to digest the products.
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bioRxiv - Microbiology 2021Quote: ... and 2U of Uracil-D-glycosylase (New England Biolabs, USA). Conditions of PCR were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... The annealed duplex was then treated with uracil glycosylase (NEB) in 1x UDG buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The annealed duplex was then treated with uracil glycosylase (NEB) in 1X UDG buffer (20 mM Tris-HCl ...
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bioRxiv - Genomics 2019Quote: ... and 6 µL Klenow (5 U/µL, NEB), and DNA blunting was carried out for 1 h at room temperature with shaking at 800 rpm ...
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bioRxiv - Evolutionary Biology 2019Quote: ... to remove Uracil residues and Endonuclease VIII (New England Biolabs M0299) to cleave DNA strands at abasic sites ...
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bioRxiv - Microbiology 2019Quote: ... The adapter loop was opened by Uracil-Specific Excision Reagent (NEB) to excise the uracil ...
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bioRxiv - Bioengineering 2020Quote: ... such that a Uracil-Specific Excision Reagent (USER, New England Biolabs) will cut the cDNA at these locations thereby releasing the desired product and permitting amplification of this product via PCR ...
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bioRxiv - Biochemistry 2022Quote: ... The USER™ (uracil-specific excision reagent) Friendly Cloning Kit (NEB) was used for all the cloning procedures ...
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bioRxiv - Synthetic Biology 2020Quote: ... by USER™ (uracil-specific excision reagent) cloning (New England Biolabs), as previously described (D’Ambrosio et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... to remove uracil residues and Endonuclease VIII (New England Biolabs M0299) to cleave DNA strands at abasic sites ...
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bioRxiv - Synthetic Biology 2022Quote: ... This reaction consisted of 0.05U/µl Uracil-DNA Glycosylase (NEB; M0280), 0.1 U/µl Endonuclease IV (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... uracil was digested by USER enzyme from NEB (Ipswich, MA, USA) in a loop structure of the adapter ...
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bioRxiv - Genetics 2024Quote: ... The samples were treated with Uracil-DNA-Glycosylase (UDG) from NEB to degrade the original uracilated template ...
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bioRxiv - Neuroscience 2020Quote: ... comprising 6 mM 5’ cap analog (New England Biolabs), 7.5 mM adenosine triphosphate and 1.5 mM guanosine triphosphate (USB ...
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bioRxiv - Molecular Biology 2019Quote: ... 16.5 µl cleared lysate and uracil DNA glycosylase (UDG; NEB, 1.25 units). Abasic site cleavage was induced by the addition of 100 mM NaOH and incubation at 95° C ...
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bioRxiv - Genomics 2019Quote: ... and the barcode information cleaved using a Uracil-Specific Excision Reagent (NEB) targeting the 5d(U ...
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bioRxiv - Biochemistry 2021Quote: MtDNA was treated with 1 μl uracil-DNA glycosylase (UDG) (NEB, M0280), 1.5 μl Endonuclease IV (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... containing uracil and overhangs compatible with Thermolabile USER II digestion/ligation (NEB). Following outPCR ...
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bioRxiv - Biochemistry 2020Quote: ... The substrate was then treated with 50 U of uracil DNA glycosylase (NEB) for 2 h at 37°C to form the abasic site ...
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bioRxiv - Cell Biology 2019Quote: ... the second strand cDNA was digested by USER (Uracil-Specific Excision Reagent, NEB) prior to selecting for fragments approximately 150-200bp in length with AMPure XP beads ...
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bioRxiv - Genomics 2020Quote: ... and 3 μL of Uracil-Specific Excision Reagent (USER) Enzyme (NEB, Beijing, China) was then used with the size-selected and adaptor-ligated cDNA at 37°C for 15 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protocol included the treatment with Uracil-DNA-Glycolase (New England Biolabs M0279) to remove Uracil residues and Endonuclease VIII (New England Biolabs M0299 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protocol included the treatment with Uracil-DNA-Glycolase (New England Biolabs M0279) to remove uracil residues and Endonuclease VIII (New England Biolabs M0299 ...
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were then treated with 0.5 U/reaction uracil DNA glycosylase (NEB, USA) for 10 min at 37°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We repaired the DNA with USER (Uracil-Specific Excision Reagent) Enzyme (NEB, USA) for deanimation ...
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bioRxiv - Genomics 2023Quote: ... and Uracil-Specific Excision Reagent (1 U/μl USER, catalog num. M5505L, NEB) treatment ...
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bioRxiv - Cell Biology 2023Quote: ... the products were treated with uracil-specific excision reagent (USER) enzyme (NEB, N5505S) to remove the forward primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we prepared new DNAlibraries adding uracil-DNA glycosylase (USER™ enzyme (New England Biolabs)) during the blunting step20 ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µg DNA was treated with uracil DNA glycosylase (New England Biolabs, Inc.) at 37 °C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... The dUTP-containing strand was degraded using Uracil-DNA Glycosylase (New England Biolabs #M0280S). The resulting single-stranded cDNA was amplified with 13 or 15 PCR cycles followed by size-selection using Pippin Prep (Sage Science) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uracil-containing strands were degraded by treatment with USER Enzyme (New England Biolabs, USA). Sequencing was performed using Illumina Novaseq 6000 Platform in paired-end format with the Phred score of 64 ...
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bioRxiv - Immunology 2021Quote: ... The sample was then treated with 0.5 units/μl of Uracil-DNA Glycosylase (NEB) for 60 min at 37°C to reduce UMI interference ...
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bioRxiv - Genomics 2020Quote: ... 0.3 uL of TSO digestion buffer containing 1U Uracil-DNA glycosylase (UDG, NEB M0280S) were added to each well ...
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bioRxiv - Genomics 2022Quote: ... the probes were subjected to uracil-specific excision reagent (USER) enzyme (NEB, Cat. N5505S) treatment for ∼24 h at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... gap repaired with HiFi HotStart Uracil+ Ready Mix (Kapa) and Taq DNA ligase (NEB), and treated with a mixture of USER enzyme and T4 polynucleotide kinase (NEB) ...