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Citations for New England Biolabs :
1 - 50 of 1638 citations for 5α CHOLESTAN 3 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... coli 5α (NEB) were used for cloning ...
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bioRxiv - Biochemistry 2023Quote: ... coli 5α cells (NEB) via heat shock and plated on LB medium containing kanamycin at a concentration of 50 μg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB-5α (NEB, C2987H) was used for plasmid propagation and was cultivated in LB Broth (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... coli NEB-5α (New England Biolabs) cells and screened by colony PCR ...
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bioRxiv - Neuroscience 2020Quote: ... coli cells (NEB 5αs, New England Biolabs). Clonal bacterial cultures were then produced ...
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bioRxiv - Neuroscience 2020Quote: ... coli cells (NEB 5αs, New England Biolabs). Clonal bacterial cultures were then produced ...
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bioRxiv - Biochemistry 2022Quote: ... 5α Competent Escherichia coli (30 μl; NEB) was then transformed with 2 μl of the assembly reaction mixture by heat shocking for 30 s at 42 °C ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB- 5α (New England Biolabs) cells were used for all cloning reactions ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB-5α (New England Biolabs) was used for MES transformation when constructing plasmids as well as for plasmid propagation ...
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bioRxiv - Biochemistry 2021Quote: ... transformed clone of NEB-5α (New England Biolabs) grown in LB and 100 μg/mL ampicillin antibiotic ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB-5α (New England Biolabs, Beverley, MA) was grown in Luria broth (LB ...
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bioRxiv - Developmental Biology 2022Quote: ... Transformations were performed using 5α-competent E.coli (New England Biolabs C2987I). To generate ES cells with Doxycycline inducible Gata4 ...
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bioRxiv - Microbiology 2022Quote: ... and transformed them into chemically competent NEB-5α cells (New England Biolabs) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligated products were transformed into NEB 5α competent cells (New England BioLabs) using the heat shock method ...
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bioRxiv - Synthetic Biology 2021Quote: Entry and expression plasmids were grown in NEB-5α cells (New England Biolabs). Destination plasmids were grown in One Shot ccdB survival cells (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The Escherichia coli strain used for routine cloning was NEB 5α (New England Biolabs). The plasmids used in this article are listed below (Table S2) ...
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bioRxiv - Immunology 2021Quote: ... the digested product transformed into NEB 5α competent cells (New England Biolabs, Ipswich-MA, USA). The mutation was confirmed by sequencing and restriction digestion.
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bioRxiv - Microbiology 2022Quote: ... coli strain used in this study for cloning purposes was NEB-5α (New England Biolabs) (Table 1) ...
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bioRxiv - Biophysics 2023Quote: ... Calmodulin and myosin-5 expression plasmids were prepared from E.coli 5α cells (New England Biolabs), transformed with the each of plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... The ligation product was introduced into NEB 5α competent Escherichia coli cells (NEB, Evry, France), transformants selected on kanamycin (50 µg.mL-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RNA Protection buffer (New England Biolabs) and RNA was isolated according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit ...
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bioRxiv - Genetics 2022Quote: The detection of aberrant Scyl1 transcripts was performed by RT-PCR using primers designed to amplify sequences between exon 2 to exon 5 (RT-Scyl1_F21 5’-CGCAGTGTCCATCTTCGTGTA-3’ and RT-Scyl1_R51 5’-CCCGGCAGTTCTGCAGGAA-3’) following the OneTaq One-Step RT-PCR Kit (New England Biolabs, E5315S) procedure ...
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bioRxiv - Microbiology 2023Quote: ... 10 μM SARS-CoV-2 E-gene Forward (5’- ACAGGTACCTTAATAGTTAATAGCGT-3’) and Reverse (5’-ATATTGCAGCAGTACGCACACA-3’) primers were used with Luna Universal One-step RT-qPCR kit (New England Biolabs) and added to 1 μl supernatant (5μl total ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... One piece of brain tissue (∼300 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Microbiology 2019Quote: ... NEB One Taq One-Step RT-PCR Kit (NEB # E5310S) was used for nucleic acid amplification ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler or an Applied Biosystems StepOnePlus system ...
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bioRxiv - Biochemistry 2021Quote: ... 5’-TAATCAGACAAGGAACTGATTA-3’ (Forward) and 5’-CGAAGGTGTGACTTCCATG-3’ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler ...
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bioRxiv - Immunology 2022Quote: ... and nCoV_IP4-14084Probe(+) TCATACAAACCACGCCAGG [5’]Hex [3’]BHQ-1 19) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB). Serial dilutions of a titrated viral stock were analyzed simultaneously to express viral loads as eqPFU per gram of tissue.
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bioRxiv - Microbiology 2019Quote: ... Individual fragments were designed that possessed 5’ and/or 3’ sequence homology to one another using Q5 high-fidelity DNA polymerase (New England Biolabs), followed by stitching the individual fragments together in a SOE reaction.
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bioRxiv - Plant Biology 2020Quote: ... epicentre cat # ER0720 or ER81050-we used this one) and A-tailing (100mM dATP; bioPioneer inc, Klenow; (3’-5’ exo- NEB) # M0212L (1,000 units ...
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bioRxiv - Plant Biology 2022Quote: ... Four vectors including one of the active 5′gRNA-pairs and one of the active 3′gRNA-pairs were digested by BglI (New England Biolabs) and ligated at once ...
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bioRxiv - Biochemistry 2023Quote: ... 5′ FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1 3′) and the Luna Universal Probe one-step RT-qPCR kit (catalog no. E3006; New England Biolabs). A 20-μL RT-qPCR mixture contained 7 μL of sample ...
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bioRxiv - Genomics 2021Quote: ... One μL APOBEC3A (NEB #E7120S) was added directly to the reaction with 10 μL of 10x APOBEC3A reaction buffer and 1 μL BSA (10 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Neuroscience 2021Quote: ... Genes of interest were amplified with the One Taq One-Step RT-PCR kit (NEB) using 1 μg of RNA ...
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bioRxiv - Microbiology 2021Quote: ... with Luna Universal One-Step Rt-qPCR & Probe One-Step RT-qPCR Kits (NEB, USA), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... One was to use a kit to generate sequencing libraries in one-tube reactions (NEB, E7103S). Another modification was to spike-in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher ...
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bioRxiv - Genomics 2023Quote: ... one female and one male HRFI) were artificially methylated using the M.Sss1 Enzyme (New England BioLabs) following the manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... One microliter of RNase H (NEB) was added to each tube ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution DNase (NEB) digestions were performed ...
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bioRxiv - Neuroscience 2022Quote: ... One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England BioLabs) in a total volume of 20 µl and a template concentration of 50 ng/µl according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... NEB One Taq RT-PCR kit (One Taq® RT-PCR Kit, New England Biolabs INC, Frankfurt, Germany) was used for cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Genomics 2021Quote: ... One microliter of QuickCIP (New England Biolabs) was added and the solution was incubated at 37 °C for 10 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One microliter of T7 endonuclease Ⅰ (NEB) was added to the sample ...
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bioRxiv - Bioengineering 2022Quote: ... and one with BseYI (NEB cat# R0635S) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL Klenow fragment (3’→5’ exo-) (NEB), and 9 μL of DEPC H2O to each reaction and incubating at 37 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs were used as templates for SYBR green-based one-step reverse-transcriptase quantitative PCR (RT-qPCR) using the NEB Luna One-Step RT-qPCR kit (New England Biolabs). All primers were validated by standard curve analysis and had PCR efficiencies ranging from 90-110% ...