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Citations for New England Biolabs :
4451 - 4500 of 10000+ citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Neuroscience 2024Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) was performed on the RNA ...
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bioRxiv - Genomics 2024Quote: ... The pooled DNA was PCR-amplified using Phusion® High-Fidelity PCR Kit (NEB®), followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®) ...
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bioRxiv - Molecular Biology 2024Quote: ... Y511A mutation in pBAD-His-BrxX was introduced using Q5 site-directed mutagenesis kit (NEB), while introduction of Y511A to the pBREX AL was carried by joining 2 plasmid fragments ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Genomics 2020Quote: ... 50-100 fmol of PCR products were diluted in 9 µl then mixed with 1 µl of Rapid 1D Adapter and 1 µl of Ligase T4 Blunt (New England Biolabs, Ipswich, MA, USA). Pre-sequencing mix was incubated 10 min at 25°C and left on ice until ready to load ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 µM of the 6-kb fragment was incubated with 1 unit/µL terminal deoxynucleotidyl transferase (TdT, New England Biolabs, MA, USA, #M0315S) and 0.5 mM deoxyadenosine triphosphate (dATP ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro methylation experiments were performed for 10 min (DNA) or 1 h (RNA) at 37 °C in 8 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc., Massachusetts, USA) with 20 nM DNA or 50 nM RNA with 1 unit RNase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified NbPRS4 PCR product was gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs), digested with XbaI and XhoI (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... The insert was further cloned into pcDNA3.4 using the HiFi DNA Assembly Kit (New England Biolabs) and PCR products (Phusion polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... GSE1 coding sequence was modified using the Q5 Site-directed Mutagenesis Kit (E0554S, New England Biolabs) to substitute the cytosine (C ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNALibrary Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was synthesized from 700ng of amplified oligos using T7 RNA polymerase (HiScribe T7 kit, NEB) following manufactural instruction ...
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bioRxiv - Cell Biology 2019Quote: ... A point mutation (D697A) was introduced with the Q5® Site-Directed Mutagenesis Kit (E0554S NEB). Yeasts were cultured to an OD600 of 4 ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg total RNA was used to synthesise cDNA with the ProtoScript II kit (New England Biolabs) using random primers in a 20μl reaction ...
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bioRxiv - Cell Biology 2020Quote: ... The sgRNAs were in vitro synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Transcription reactions were treated with RNase-free DNaseI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and circularized by an intramolecular ligation reaction using the Quick Ligation™ Kit (New England Biolabs)
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bioRxiv - Immunology 2021Quote: ... the mRNA was synthesized following the HiScribeTM T7 ARCA mRNA Kit (with tailing) (New England Biolabs). Once the mRNA was synthesized ...
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bioRxiv - Genetics 2019Quote: ... Libraries for sequencing were prepped using an NEBNext Ultra II Library prep kit for Illumina (NEB) and loaded for paired-end sequencing using the Illumina HiSeq 1500.
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bioRxiv - Immunology 2021Quote: ... Site-directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Constructs were verified by Sanger DNA Sequencing at the London Regional Genomics Center ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Alternative splicing analysis of RNA sequencing data was completed using the Vertebrate Alternative Splicing and Transcript Tools (VAST-TOOLS ...
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bioRxiv - Molecular Biology 2021Quote: ... The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and paired-end sequenced on an Illumina MiSeq.
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bioRxiv - Developmental Biology 2021Quote: The resulting PCR fragments were gel purified using the Monarch Gel Purification Kit (New England Biolabs) and TA cloned into pGEM-T Easy (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Illumina library preparation was performed with this pool using the NEBNext Ultra kit (NEB, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), and sequenced on an Illumina Novaseq PE150 platform ...
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bioRxiv - Microbiology 2021Quote: ... SMU_313 with mutated SmsR4-binding site was constructed using the Q5 Mutagenesis Kit (New England Biolabs) and mutations were confirmed through Sanger sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were in vitro transcribed using the HiScribe T7 Quick High Yield RNA synthesis kit (NEB) using 75 ng of template ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by mutation of the MAO binding site using the Q5 site directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The cleaved DNAs were subjected to A-tailing using NEBNext Ultra II End Prep kit (NEB) and followed by ligation of an annealed hairpin adaptor (5’-phos-CGGTGGACCGATGATC/ideoxyU/ATCGGTCCACCG*T-3’ ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, MA, E6300S) using PolyDT primers ...
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bioRxiv - Physiology 2022Quote: ... All mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...
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bioRxiv - Molecular Biology 2022Quote: ... gel purified and in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB) in a 20 μl reaction for 10 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by IDT and converted into sgRNAs using the EnGen Cas9 sgRNA kit (NEB) according to instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... we introduced a 6-bp mutation using the Q5 site-directed mutagenesis kit (New England Biolabs). The pUAST-attB constructs were inserted into either attP40 or attP86Fb (for Piezo constructs ...
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bioRxiv - Neuroscience 2021Quote: RNA was collected from cultured granule cells using the Monarch® Total RNA Miniprep Kit (NEB). Then cDNA was reverse transcribed using SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Library amplification: Amplification was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... or the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, USA) was used to transcribe the templates ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq experiments were performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2020Quote: ... cDNA was generated using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB (UK Ltd.), Hitchen ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) with NEBNext Multiplex Oligos (New England Biolabs) ...