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Citations for New England Biolabs :
3301 - 3350 of 10000+ citations for Glutathione Fluorescent Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs; #E0554S). Guide RNA and repair template sequences are listed in Supplementary Data 3c.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer’s instructions and the libraries were sequenced at 50 bp read length and were sequenced on an NovaSeq SP with a target of ∼40M PE reads per sample.
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bioRxiv - Cancer Biology 2024Quote: ... using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer’s instructions and sequenced using Hiseq 4000 PE 150bp ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Library Quant Kit for Illumina (New England BioLabs) according to the instructions of the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... Libraries were constructed using the NEBNext Enzymatic Methyl-seq Kit (NEB), following the manufacturer’s guidance ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBnext Ultra II Directional RNA library prep kits (NEB #E7760L). Paired end 200 bp sequencing with additional reads for dual 8/8 indices plus the 11nt UMI after the i7 index was performed on an Illumina NovaSeq 6000 at the University of Chicago Genomics Facility.
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start Taq DNA Polymerase kit (New England Biolabs) was used according to the manufacturer’s protocol to amplify the SEGS-1 region from DNA samples collected from 4 plants of each genotype ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid extraction and DNA purification were performed using Monarch kits (NEB). Sequences were verified with Sanger sequencing by Azenta Life Sciences or Primordium Labs for whole plasmid sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... and then quantified by qPCR (NEBNext Library Quant Kit, NEB, E7630). 20 pM of DNA were loaded onto an Illumina MiSeq (v3 ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was used with the EpiMark Bisulfite Conversion Kit (NEB) using the manufacturer instructions.
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bioRxiv - Immunology 2024Quote: ... and gRNAs were purified with the Monarch RNA Cleanup Kit (NEB), aliquoted ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and Biolux Cypridina luciferase assay kit (New England Biolabs, USA; #E3309), respectively ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA was extracted using Monarch Genomic DNA Purification Kit (NEB) according to manufacturer’s instructions and eluted in 20 µL water.
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bioRxiv - Cell Biology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554), the DpnI (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Monarch® PCR & DNA Cleanup Kit (NEB). An aliquot (1 µg ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (NEB), and quality checked using a genomic Tapestation 4200 (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... using the 2X Gibson Assembly Kit (Cat # E2611S, New England Biolabs). Assembled constructs were confirmed by whole plasmid sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2024Quote: ... using the NEBNext Ultra II DNA Library prep Kit (E7370; NEB). Libraries were sequenced using an Illumina NextSeq 2000 sequencer 75 bp Single End.
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bioRxiv - Microbiology 2024Quote: ... Gibson Assembly® Cloning Kit was employed (New England BioLabs Inc) to make the deletion construct pEX-18Tc-femI ...
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bioRxiv - Microbiology 2024Quote: ... and cloned into pLGB13 using the NEBuilder HiFi cloning kit (NEB). The pLGB13 plasmid was linearized by double digest with BamHI and SalI restriction enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... dsRNA was purified using Monarch RNA Cleanup Kit (50 μg; NEB) and checked on 1% agarose gel and concentration measured with a NanoDrop One spectrophotometer (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... or the Monarch® DNA Gel Extraction Kit (New England Biolabs). In a few cases ...
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bioRxiv - Systems Biology 2022Quote: ... 100ng of vector backbone and 20ng of trcrRNA-CaptureSeq-5’LTR(truncated) cassette were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...
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bioRxiv - Plant Biology 2019Quote: ... A portion of leaf tissue (approximately 5 mm2) was homogenized by grinding in 50 μL of 1X Q5 reaction buffer (New England Biolabs Cat. No. B9027S) in a 1.5 mL microcentrifuge tube with a micropestle ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of 5 μl of UDG reaction solution (New England Biolabs, 0.02 U μl−1 UDG in 1X UDG buffer) and an additional 30 min incubation at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... A 3’ A overhang was added to the ends of the blunted DNA fragment with Klenow Fragment (3’-5’ exo; NEB; Cat. No. M0212L). The DNA fragments was then ligated with diversity-increased custom barcodes (Shi et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was slowly cooled down to room temperature with a Δ -1°C / second gradient and 0.5 μl of each RNase H (NEB, 5 U/μl), RNase T1 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... cDNA (2.5 μL) was amplified in two multiplexed PCR reactions using Q5 Hot-Start DNA High-fidelity Polymerase (New England Biolabs, Ipswich, MA, USA) using the following cycling conditions ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was analyzed in duplicate 10-µl reactions containing 5 µl Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA), 2 µM of each primer ...
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bioRxiv - Molecular Biology 2019Quote: ... a volume of 0.5 µl of the respective restriction enzyme BbsI (5 units; ThermoFisherScientific, Waltham, US) or BsaI-HF®v2 (10 units; New England Biolabs, Ipswich, US) and then 1 µl (1-3 units ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA (2.5 μL) was amplified in two multiplexed PCR reactions using Q5 Hot-Start DNA High-fidelity Polymerase (New England Biolabs, Ipswich, MA, USA) using the following cycling conditions ...
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bioRxiv - Genomics 2021Quote: ... followed by with a buffer adapted from (5-9) consisting of 0.1% (1mg/mL) BSA (New England Biolabs, Ipswich, MA, Item No. B9000S) / 0.3% Igepal CA 630 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... The single stranded cDNA was ligated with a partial Illumina 5’ adaptor (HZG885:/5phos/AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTddC) using T4 RNA ligase 1 (New England Biolabs, Ipswich, MA, US) and incubated overnight at 22 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ssDNA standards were generated from PCR products amplified with a forward primer that was 5’ phosphorylated to promote Lambda exonuclease digestion of that strand (NEB, Cat. No. M0262S) and a reverse primer with phosphorothioate bonds between the first four 5’ nucleotides to block digestion ...
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bioRxiv - Microbiology 2024Quote: ... The initial dephosphorylation reaction was in a mixture (50 μL) containing 5 μL of terminal transferase buffer (NEB Tdt Reaction Buffer, Catalog # M0315S), 1 μL of shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Microbiology 2024Quote: ... The initial dephosphorylation reaction was in a mixture (50 μL) containing 5 μl of terminal transferase buffer (NEB Tdt Reaction Buffer, Catalog # M0315S), 1 μL of shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl of the dissolved cells were added to a 50 µl PCR reaction (Q5 DNA polymerase, NEB; see Table S2 for primer sequences) and amplified with 36 rounds of thermal cycling ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA (400 ng) was either mock digested (i.e. without enzyme addition) or digested with BglII (New England Biolabs, 5 units/rxn, NEBuffer 3.1) for 1 hour at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... The resulting product (typically 50 – 100 μL of 5 – 20 μM of protein) was mixed with an equimolar amount of SNAP-Surface 549 (New England Biolabs; 1 mM in DMSO) and incubated at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x, MgCl2 5 mM, 0.5 µl NEB nuclease P1, 0.5 µg benzonase) samples were incubated over night at 37 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng purified linear vector fragments were assembled with 5 µl of annealed oligonucleotide pool using NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA, USA, #E2621L) according to the manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... and for the synthesis of the second strand of cDNA was used the Klenow fragment 3’-5’ exo (New England Biolabs Inc., Ipswich, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... ChIP DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... were mixed for DNA assembly using the Golden Gate assembly kit (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit (NEB), modified for CUT&RUN as previously described(54) ...
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bioRxiv - Cell Biology 2019Quote: ... then purified with Monarch PCR & DNA Purification Kit (New England BioLabs, T1030S) followed by T4 DNA Ligase reaction (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... and the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760). The libraries were sequenced using Next-Seq with single-end ...