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Citations for New England Biolabs :
2751 - 2800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... was mixed with 1.75 μl of Ultra II End-prep Reaction Buffer and 1.5 μl of Ultra II End-prep Enzyme Mix (cat # E7546, New England Biolabs MA, USA) and incubated at 20°C for 5 min ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 0.2 U/µL RNase Inhibitor (NEB cat. #M0314L). A gentleMACS Octo Dissociator (Miltenyi Biotec cat ...
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bioRxiv - Genomics 2024Quote: ... Incompletely formed SMRTbell templates were removed by digestion with a combination of exonuclease III and exonuclease VII (NEB, MA). The qualification and quantification of the SMRTbell libraries were made on a Qubit fluorimeter (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... 27% second strand synthesis buffer (NEB), and 13% second strand synthesis enzyme mix (NEB ...
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bioRxiv - Genetics 2024Quote: ... then resuspended and incubated in 20uL digestion buffer (1.1mL 1M Tris-HCl of pH6.8 in 50mL deionized water) with 2uL proteinase K (New England Biolabs) at 37°C for 15min ...
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bioRxiv - Genetics 2024Quote: In vitro transcription was carried out on a linear DNA template using the HighScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... followed by HiFi cloning (NEB) to generate pzf8 ...
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bioRxiv - Genetics 2024Quote: Wild-type minigene plasmids were prepared using target exons and variable flanking intronic sequences of the DMD gene amplified from a control genomic DNA using Q5 High-Fidelity DNA Polymerase (NEB, USA). The primers that were used are described in Table S1 ...
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bioRxiv - Genetics 2024Quote: ... The PCR products were cloned into the pSpl3-Flu2-TK-del vector using Gibson Assembly Master Mix (NEB, USA).
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bioRxiv - Genetics 2024Quote: ... and after phenol-chloroform extraction the resulting fragments were ligated with T4 ligase (NEB) for 2 hours at room temperature to circularize them ...
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bioRxiv - Genomics 2024Quote: ... Amplicons were then barcoded for NGS by combining 2 µL of amplicon from the previous PCR with 1X Q5 High-Fidelity Master Mix (New England Biolabs M0492) and 500 nM forward and reverse indexing primers (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Genomics 2024Quote: The libraries for sequencing were made with NEBNext DNA library Prep Master Mix Set for 454 (E6070, New England Biolabs) and with Illumina-specific adaptors [54] using established protocols [54–56] ...
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bioRxiv - Genomics 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (Dual Index Primers, NEB) following standard protocols ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB#7490) and NEBNext Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genomics 2024Quote: ... The library was constructed with PstI/MspI enzyme combination (NEB, Ipswich, MA, USA) and the Illumina TruSeq HT Library Preparation Kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... we added 10 µL of DpnII (NEB, 50 U/μl) and continued the incubation until the end of the day when another 10 μl DpnII was added to each sample to digest overnight ...
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bioRxiv - Genomics 2024Quote: ... and NEBNext High-Fidelity 2x PCR Master Mix (NEB) for 12 cycles to generate each library ...
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bioRxiv - Genomics 2024Quote: ... Fifteen microliters of each RNA was converted to cDNA using Protoscript II First Strand cDNA synthesis (NEB) and random primer 6 (NEB) ...
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bioRxiv - Genomics 2024Quote: Libraries for metagenomic sequencing of CS2022-0121 and F16-31-UTM were prepared using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) to elucidate the enrichment efficiency of the target capture sequencing ...
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bioRxiv - Genetics 2024Quote: ... digested with restriction enzyme HpaII (NEB) and after phenol-chloroform extraction the resulting fragments were ligated with T4 ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... Purified product was Gibson cloned into a previously described PiggyBac transposon vector (37) using standard protocols in a 10 μl reaction (NEB E2621S). 2.5 μl of the Gibson reaction was electroporated into 25 μl of 10-beta electrocompetent E ...
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bioRxiv - Genomics 2024Quote: ... adapter ligation was conducted using the NEBNext Quick Ligation Module (New England Biolabs UK, Catalogue number: E6056S), with the library cleaned using AMPure XP Beads and the long-fragment buffer provided with the ONT library construction kit ...
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bioRxiv - Genomics 2024Quote: ... The pool was PCR amplified using Q5 polymerase (NEB M0492S) for 8 cycles with primers that contained overhangs for subsequent cloning ...
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bioRxiv - Genomics 2024Quote: ... using the barcodes provided and the NEB Blunt/TA Ligase Master Mix (New England Biolabs UK, Catalogue number: M0367L), with samples then being pooled together and cleaned as before ...
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bioRxiv - Genomics 2024Quote: ... Two 50 μl PCR reactions were performed per sample with Q5 Polymerase (NEB M0492S) with real-time tracking using SYBR green dye with the following cycling parameters 98°C - 3 min ...
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bioRxiv - Genomics 2024Quote: ... 10 µl of 2% BSA (NEB, B9000S, 0.01% final) and 930 µl of nuclease-free water and supplemented with 1U/ml Protector RNase inhibitor (Millipore Sigma ...
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bioRxiv - Genomics 2024Quote: ... 400 ng DNA was aliquoted per barcoded sample for end and single-strand nick repair using NEBNext Ultra II End repair/dA-tailing Module and NEBNext FFPE Repair Mix (New England Biolabs UK, Catalogue numbers: M6630S, E7546S), with samples then being cleaned using AMPure XP Beads (Beckman Coulter UK ...
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bioRxiv - Genomics 2024Quote: ... T7 IVT was performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) in a 60 μl total reaction per sample ...
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bioRxiv - Genomics 2024Quote: ... Four 50 μl PCR reactions were performed per sample with Q5 Polymerase (NEB M0492S) with the following cycling parameters ...
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bioRxiv - Genomics 2024Quote: ... 1% BSA (NEB, B9000S), and 1U/ml Protector RNase Inhibitor (between 100-200 μl depending on pellet volume) ...
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bioRxiv - Genomics 2024Quote: ... 2 μl of Antarctic Phosphatase Enzyme (NEB), and 2 μl of water ...
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bioRxiv - Genomics 2024Quote: ... 4 μl of Inorganic Pyrophosphatase (NEB) and 2 μl of Phi29 DNA Polymerase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... and then 2 μl of T7 Endonuclease (NEB) was added ...
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bioRxiv - Genomics 2024Quote: ... any residual linear DNA is digested using Exonuclease V (NEB). At this point ...
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bioRxiv - Genomics 2024Quote: ... and 2 μl of Phi29 DNA Polymerase (NEB). The reaction is incubated for 8 hours at 30°C.
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bioRxiv - Genomics 2024Quote: ... Large (Klenow) fragment without exonuclease activity (exo-) (NEB) in combination with dATP or dGTP ...
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bioRxiv - Genomics 2024Quote: ... 5 μl of CutSmart 10x (NEB), 2 μl of BSA (NEB) ...
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bioRxiv - Genomics 2024Quote: Methylation enrichment of cfDNA was performed using the EpiMark Methylated DNA Enrichment Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 5 μM each) from IDT were annealed in 1x New England Biolabs (NEB) buffer 2 (20 μl in total) ...
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bioRxiv - Genomics 2024Quote: The reaction is supplemented with 2 μl Exonuclease V (NEB) and 5 μl of ATP 10 mM ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries for regular cfDNA were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... the cfDNA can be de-phosphorylated by adding 6 μl of Antarctic Phosphatase buffer 10x (NEB), 2 μl of Antarctic Phosphatase Enzyme (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 2.5 μl of DTT and 2 μl of Blunting Enzyme (NEB) in a total reaction volume of 50 μl ...
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bioRxiv - Genomics 2024Quote: ... the reaction is treated with 2 μl of Proteinase K (NEB). The digestion is carried on at 37°C for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... dTTP (NEB), and 10 U Klenow exo− (M0212L ...
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bioRxiv - Genomics 2024Quote: For a typical blunting reaction 5-50 ng of cfDNA are mixed with 5 μl of CutSmart 10x (NEB), 5 μl of dNTPs 1mM each ...
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bioRxiv - Genomics 2024Quote: ... 2.0 U of Proteinase K (New England Biolabs) was introduced to the reaction mixture and incubated at 50°C for 1 hour to stop the oxidation process ...
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bioRxiv - Genomics 2024Quote: ... IVT reactions were set up in 30 μl total volume with the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) with 2 μl of each NTP ...
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bioRxiv - Genomics 2024Quote: ... 2 μl of BSA (NEB), 15 μl of dNTPs 10 mM ...