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Citations for New England Biolabs :
2701 - 2750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The cDNA was then used as input for the first-round PCR with oJL105 and oJL106 to add the i5/i7 primer sites using Q5 DNA polymerase (NEB M0491L). Libraries were amplified with an initial 98 °C denaturation step for 30 s ...
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bioRxiv - Genomics 2024Quote: ... Reverse transcription was performed with dT priming using the ProtoScript II RT (NEB M0368L) as described by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... and half the elution was used as a template in a second round of 8-10 cycle PCR to prepare Illumina libraries using NEBNext multiplex oligos using the same conditions described above (NEB E7335L and E7500L). The final PCR product was purified using AMPure XP beads (Beckman Coulter A63882 ...
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bioRxiv - Genomics 2024Quote: ... and 2 U/µl Thermolabile Exonuclease I (New England Biolabs, catalog no. M0568S) and incubated for 15 min at 37 °C on a shaking thermoblock at 400 rpm ...
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bioRxiv - Genomics 2024Quote: ... samples containing a mixture of single-cell ATAC and RNA libraries were resuspended in the pre-amplification mastermix containing 1x NEBNext HF 2x PCR Master Mix (NEB, catalog no. M0541L) and a combination of three primers ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing either rpsL-cat or the inducible promoter tetO1 [18] flanked by regions homologous to the DNA up- and downstream of the ggt-promoter were constructed using a commercially available Gibson Assembly Cloning Kit (NEB). Homologous regions were amplified from the genomic DNA of strain PMSS1 using primer pairs FlgK fwd/FlgK rev (rpsL/tetO1 ...
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bioRxiv - Microbiology 2024Quote: ... All three fragments were purified and combined in a Gibson assembly (New England Biolabs [41]). Sequencing revealed a point mutation in the cd3284 open reading frame ...
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bioRxiv - Microbiology 2024Quote: ... Single-construct plasmids expressing A-subunits were constructed via restriction digestion (NdeI and PstI, New England Biolabs) and ligation using pBAD24 (Amp+ ...
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bioRxiv - Microbiology 2024Quote: ... Chimeras were cloned in plasmid pSHU1234 using restriction digestion with NdeI and PstI (New England Biolabs). Plasmids carrying chimeric constructs were used as templates to generate the double-stranded donor DNA used to replace the motA and motB gene on the RP437 chromosome with pomA and potD variants ...
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bioRxiv - Immunology 2024Quote: ... 2μl of endo H (1000U) (New England Biolabs) or 2μl of PBS (mock ...
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bioRxiv - Immunology 2024Quote: ... mRNA used for comparison in NAGE gels was produced using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The PCR amplicon was purified by sequential gel extraction (1.5% agarose gel prepared in 0.5x TAE) and affinity column chromatography (Monarch® PCR & DNA Cleanup Kit, New England Biolabs). For IVT synthesis ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of 2000 U/mL DNase I (New England Biolabs) was added to bring the final reaction volume to 10 µL ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNAs were then further purified using Monarch® RNA Cleanup Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of sample from an individual cDNA reaction was mixed with 12.5 µL of 2x Q5® High-Fidelity Master Mix (New England Biolabs), 0.5 µL of each primer (0.2 µM final concentration of each primer) ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using the NEBNext Ultra II Directional RNA kit (New England BioLabs, Ipswich, MA) and sequenced with an NovaSeq 6000 at the Vanderbilt University Medical Center genomics core (VANTAGE).
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bioRxiv - Immunology 2024Quote: ... Template DNA digestion was carried out at 37 °C for 30 minutes in a thermocycler block prior to purification of the RNA using a Monarch RNA Cleanup Kit (New England Biolabs). Purified mRNA was eluted in DEPC-treated water and stored at -80 °C prior to use ...
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bioRxiv - Immunology 2024Quote: ... and 2 µL of 2000 U/mL DNase I to the IVT reaction mixture (New England Biolabs). Template DNA digestion was carried out at 37 °C for 30 minutes in a thermocycler block prior to purification of the RNA using a Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... isolated RNA samples were treated with RNase-free DNase I (New England Biolabs) to remove any contaminant DNAs which might yield false positives in downstream PCR tests ...
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bioRxiv - Immunology 2024Quote: cDNAs were synthesized from isolated RNA samples using ProtoScript II First Strand cDNA Synthesis Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: The gRNA libraries were amplified from each gDNA sample across 100 μL PCR reactions using Q5 hot start polymerase (NEB, M0493) with 1 μg of gDNA per reaction ...
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bioRxiv - Genomics 2024Quote: ... Tagmented DNA was then amplified with Phusion high-fidelity PCR master mix (NEB) using 14 PCR cycles ...
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bioRxiv - Genomics 2024Quote: ... using the Monarch DNA purification kit (New England Biolabs, Inc., Rowley, MA, USA) to extract genomic DNA from the original frozen whole blood samples ...
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bioRxiv - Genomics 2024Quote: ... coli Lemo21 (DE3) (New England Biolabs), which was grown at 30°C in lysogeny broth with 30 mg L-1 chloramphenicol and 50 mg L-1 kanamycin until an optical density (600 nm ...
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bioRxiv - Genomics 2024Quote: ... PAP enzyme and ATP (NEB, M0276L) were also added to the cell mix to facilitate in vitro polyadenylation ...
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bioRxiv - Genomics 2024Quote: ... 20 µL of 2% BSA (New England Biolabs, catalog no. B9000S), and 1.86 mL of nuclease-free water ...
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bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: In vitro transcription was carried out using HiScribe® SP6 RNA Synthesis Kit (NEB, E2070S) in a 25 μl reaction containing 1 x SP6 Reaction Buffer ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Genomics 2024Quote: ... The library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina-compatible y-adaptor ...
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bioRxiv - Genomics 2024Quote: DNA templates for in vitro transcription of λRNA were generated from a defined region of λDNA (NEB, N3011S) through PCR amplification ...
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bioRxiv - Genomics 2024Quote: ... Poly(A) mRNA was selected using Oligo d(T)25 Magnetic Beads (NEB, S1419S) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Library amplification (10-12 PCR cycles) was carried out using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs), with IDT for Illumina UD Indexes (96x ...
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bioRxiv - Genomics 2024Quote: ... A library for short-read sequencing was prepared using an NEBNext Ultra II DNA Library Prep kit (New England Biolabs, Beverly, MA) and sequenced using a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2024Quote: ... 30 µL of the eluted DNA samples were mixed with 7 µL of TSO digestion mix (3.5 µL UDG-DNA glycosylase and 3.5 µL UDG Buffer, NEB M0280S) and incubated at 37 ºC for 30 min ...
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bioRxiv - Genetics 2024Quote: ... 2 mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Genomics 2024Quote: ... and 5 units of EPAP (NEB, M0276L). The reaction was incubated at 37°C for 5 min ...
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bioRxiv - Genomics 2024Quote: ... together with the low range ssRNA ladder (NEB, 0364S).
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bioRxiv - Genomics 2024Quote: ... the DNA fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were constructed using Illumina Nextera Dual Indexing ...
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bioRxiv - Genomics 2024Quote: Selected genomic regions of range 175-200 bp were synthesized using GeneArt Strings DNA fragments along with restriction enzyme sites for with Kpn1 (NEB, R0142) and XhoI (NEB ...
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bioRxiv - Genomics 2024Quote: ... Poly-A selected libraries were prepared with a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced using the NovaSeq 6000 (Ilumina ...
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bioRxiv - Genomics 2024Quote: ... and XhoI (NEB, R0146) to clone in pNL3.2 vector ...
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bioRxiv - Genomics 2024Quote: ... were amplified by PCR and cloned at the ClaI site in the Rosa26-targeting vector pEN111 by Gibson assembly (New England Biolabs). The resulting vectors obtained (pEN111-TRE3G-LKF/AKF-IRES-ZsGreen1-rtta3G_Rosa26-donor ...
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: ... A combination of 48 i7 oligos (NEBNext Multiplex Oligos for Illumina, NEB) and 24 i5 oligos (Microsynth ...
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Genomics 2024Quote: ... in the presence of 1% VRC (Vanadyl Ribonucleoside Complex; NEB, S1402S). Coverslips were then rinsed 3 times in 70% EtOH and stored at -20C ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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bioRxiv - Genomics 2024Quote: ... RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog #: E7760, New England Biolabs Inc.), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: The custom double stranded adapter containing primer-binding site was ligated by mixing 10 μl of NEB TA/Blunt Ligase Master Mix (cat #M0367, New England Biolabs MA, USA), 2 μl of duplex adapter (1 μM ...