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Citations for New England Biolabs :
2651 - 2700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... extension 15s) genes for 30 cycles using OneTaq DNA Polymerase (NEB, M0480L).
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bioRxiv - Microbiology 2024Quote: ... sticky-end annealed sgRNAs were ligated into a BbsI-HF (NEB, R0539S) digestion of pT7sgRNA ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot of 1 A260 unit of ribosome-bound mRNA fraction was digested with 800 units Micrococcal nuclease (NEB) for 20 min at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... The resulting GlnA1_opt PCR-product and vector pRS375 were restricted with NdeI and BamHI (NEB, Schwalbach, Germany); the resulting pRS375 vector fragment and the GlnA1 fragment were ligated resulting in pRS1841 ...
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bioRxiv - Microbiology 2024Quote: ... which was linearized with BbsI (R3539, NEB, USA). The p3xFlag-GGCX (Flag-GGCX ...
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bioRxiv - Microbiology 2024Quote: ... acidocaldarius DSM 639 wild type using the primers (Eurofins Genomics) listed in supplementary Table 4 employing the Q5 polymerase (NEB, USA) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... PCR products and the respective antibiotic resistance gene were used for Gibson assembly (NEB, USA) (38) ...
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bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of D072 primer (Table 1) and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 μCi) ...
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bioRxiv - Microbiology 2024Quote: ... Polyadenylated RNA was then isolated from the whole-cell RNA using NEB Oligo d(T)25 Magnetic beads (NEB, S1419S) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2024Quote: ... Samples were DNase I (NEB, M0303) treated for 20 mins at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 200U/ml murine RNase inhibitor (NEB, M0314)) ...
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bioRxiv - Microbiology 2024Quote: ... The backbone fragments were generated using Q5 high fidelity polymerase (NEB, M0491), treated overnight with DpnI and the DNA was purified using MicroElute Cycle-Pure Kit (Omega Bio-Tek) ...
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bioRxiv - Microbiology 2024Quote: ... L742A and L786A mutations were inserted into pACEBac1-Strep-Psc-BICD2711-800 (either individually or in combination) by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). The full-length mouse BICD1 sequence (UniProt Q8BR07 ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM DTT and 100 μM ATP) and incubated with 10 μM SNAP-Cell TMR-Star dye (NEB) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A fragment containing the CRISPR array plus the extra BsrG1 restriction site was cut from the synthesized pUC19 by digestion with DraI and BstZ17I enzymes and cloned into the BsaI-linearized pSTU-1 plasmid using the NEBuilder HiFi cloning kit (New England Biolabs, UK). The plasmid sequence was confirmed by sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... were digested with NotI and the wc-1 and wc-2 fragments were cloned into the respective plasmids with NEB HiFi DNA assembly mastermix (NEB, US). This resulted in the prey plasmid wc-1-pMB29 and bait plasmid wc-2-pMB28 ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x r3.1 buffer (NEB), 2 µl of 100µM DTT and 1 µl of NudC (M0607S ...
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bioRxiv - Microbiology 2024Quote: ... PCR product was then directly used for circularization with KLD enzyme mix (NEB) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... and 2 µg/µL of Proteinase K (New England Biolabs, #P8107S), was prepared and loaded into a 3 mL syringe (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... DNA was then amplified using NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) for 5 cycles ...
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bioRxiv - Immunology 2024Quote: ... DNA libraries were then prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequencing adaptors were ligated using NEBNext Multiplex Oligos for Illumina (#E7500S ...
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bioRxiv - Immunology 2024Quote: ... The poly(A) mRNA isolation was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). The mRNA fragmentation and priming were performed using NEBNext First Strand Synthesis Reaction Buffer and NEBNext Random Primers ...
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bioRxiv - Immunology 2024Quote: ... Second round nested-PCR reactions were performed using Phusion proof reading polymerase (NEB). A third round of PCR was performed with HotStar Taq DNA polymerase master mix (Qiagen ...
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bioRxiv - Immunology 2024Quote: PBMCs were co-emulsified with oligo d(T)25 magnetic beads (New England Biolabs) in lysis buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... with 10 × DNase I buffer diluted in water (New England Biolabs #M0303S). Samples were incubated in the thermocycler for 30 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: One µg of SQBs was incubated with 1.0 U/µL DNase I (NEB) with 10 × DNase I buffer diluted in water (New England Biolabs #M0303S) ...
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bioRxiv - Immunology 2024Quote: ... or a low input method using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB, Cat. No. 7530). First-strand cDNA synthesis and tailing by reverse transcription were performed using SMART (Switching Mechanism at 5’ End of RNA Template ...
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bioRxiv - Immunology 2024Quote: ... plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S, New England Biolabs).
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bioRxiv - Immunology 2024Quote: Libraries for sequencing were prepared using extracted RNA and the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (New England Biolabs). Quality of all RNA and library preparations were evaluated with BioAnalyzer 2100 (Agilent).
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bioRxiv - Molecular Biology 2024Quote: ... PNK treatment (NEB) for 20 min at 37°C and reannealing of the RT-primer (8 μM) ...
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bioRxiv - Immunology 2024Quote: ... and 2mM Vanadyl-ribonucleoside-complex (New England Biolabs).
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bioRxiv - Immunology 2024Quote: ... 2mg/ml BSA (New England Biolabs), and 2mM Vanadyl Ribonucleoside Complex (VRC ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested plasmid and the hybridised oligonucleotides were ligated using the T4 ligase (NEB) and then transformed in Escherichia coli HST08 competent cells (Takara) ...
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bioRxiv - Molecular Biology 2024Quote: ... After DNase I digestion (NEB, cat # M0303L), cDNA was synthesized from 1µg RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Table S4) were hybridised by slow cooling down from 95-25°C and then phosphorylated using the T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridised oligonucleotides were ligated using the T4 ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... RNase H (NEB, cat.m0297, USA) and dUTP Solution (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... truncated KQ (M0373L, New England Biolabs) (Mito et al. ...
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bioRxiv - Microbiology 2024Quote: ... the mRNA was fragmented into short fragments using divalent cations under elevated temperature (Magnesium RNA Fragmentation Module (NEB, cat. e6150, USA) under 94℃ 5-7min) ...
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bioRxiv - Microbiology 2024Quote: ... and ISG65N188A,H189A,Y190A were generated using Gibson Assembly (NEB) and expressed and purified as described for ISG65 24-385 above ...
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bioRxiv - Neuroscience 2024Quote: ... The rRNA-depleted samples were reverse-transcribed with ProtoScript II (M0368L, New England Biolabs), circularized with CircLigase II (CL9025K ...
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bioRxiv - Microbiology 2024Quote: ... coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB ...
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bioRxiv - Microbiology 2024Quote: ... After the heat-labile UDG enzyme (NEB, cat.m0280, USA) treatment of the U-labeled second-stranded DNAs ...
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bioRxiv - Neuroscience 2024Quote: ... and PCR-amplified using Phusion polymerase (M0530S, New England Biolabs) (Mito et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... which was digested with the restriction enzymes EcoRI-HF (R3101S, NEW ENGLAND BioLabs) and AgeI-HF (R3552S ...
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bioRxiv - Cancer Biology 2024Quote: ... and AgeI-HF (R3552S, NEW ENGLAND BioLabs). Plasmids containing shRNA or corresponding empty vector were cotransfected with lentivirus packaging plasmids (pLP1 ...
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bioRxiv - Bioengineering 2024Quote: ... coli T7SHuffle (NEB). pET28a and pETDuet constructs were transformed into chemically competent E ...
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bioRxiv - Bioengineering 2024Quote: Constructs were cloned by PCR methods using Q5 High-Fidelity Polymerase (New England Biolabs) and fragments were assembled by Gibson assembly ...
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bioRxiv - Bioengineering 2024Quote: ... coli T7 Express (NEB). Colonies were picked into 10 mL LB containing either 100 µg/mL ampicillin (pDEST14/pETDuet ...