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Citations for New England Biolabs :
2501 - 2550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and used in a 1:1:1 molar ratio Golden Gate assembly reaction with BsaI (NEB) and T4 ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... NEBuilder DNA HIFI assembly (New England BioLabs), or ligation independent cloning (pLATE31) ...
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bioRxiv - Microbiology 2024Quote: ... A 10 μL volume of 10-fold DNAse I reaction buffer (NEW ENGLAND BioLabs, catalogue-B0303S), 5 units (2.5 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclear soluble (NEB), chromatin-bound regions (ChrB) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting eluant was further treated with 1% SDS and 8 U/mL proteinase K (NEB, P8107S) at 42°C for 20 minutes then phenol chloroform extracted and ethanol precipitated.
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bioRxiv - Molecular Biology 2024Quote: ... Purified in vitro transcribed RNA was capped using the Vaccinia Capping System (NEB, M2080S) according the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and digested with BsmBI-HF (NEB) at 55°C for 3h followed by purification using the Zymo kit ...
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bioRxiv - Microbiology 2024Quote: ... and AvrII (NEB #R0174S) restriction endonucleases to allow for insertion of TgVEG_PIGJ by homology directed ligation ...
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bioRxiv - Microbiology 2024Quote: ... isolated DNA from MPA/xanthine selected parasites was PCR amplified using GRA6 primers and the purified amplicon was fragmented with MseI (NEB cat# R0525M). Products were run on a gel to determine parasite type I or III based on unique fragmentation sizes indictive of the genotype.
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bioRxiv - Microbiology 2024Quote: The pLIC-HA-DHFR-TS (gift from Jeroen Saeij, University of California, Davis) plasmid was treated simultaneously with PacI (NEB #R0547S) and AvrII (NEB #R0174S ...
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bioRxiv - Microbiology 2024Quote: ... and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB, cat# E5520) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was incubated with EcoRV (NEB) that cuts inside mCherry for 30 min at 37°C to linearize any remaining template DNA ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the annealed oligos were phosphorylated in a 10 µl reaction containing 0.25 µl T4 PNK (10000 units/ml, NEB) and 1 µl 10x T4 ligase buffer for 40 min at 37°Ϲ before heat-inactivation at 65°Ϲ for 20 min ...
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bioRxiv - Microbiology 2024Quote: Plasmid pET28bR109H for overexpression of the R109H mutant was obtained by site-directed mutagenesis of plasmid pET28bRho (kindly provided by Pr. James Berger, Johns Hopkins University) using the NEBuilder HiFi kit (New England Biolabs). The R109H mutant was overexpressed in BL21(DE3)pLysS cells carrying the pET28bR109H plasmid and purified as described for WT Rho 105.
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bioRxiv - Microbiology 2024Quote: ... coli RNA polymerase (0.4 pmol; New England Biolabs), Rho (1.4 pmol) ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Strains were typically constructed by PCR amplifying vector and insert fragments with compatible 25-30 bp overhangs and ligating them by Gibson assembly (NEB). For the alt.-3-targeting pCas9 construct ...
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bioRxiv - Microbiology 2024Quote: ... and BsaI-v2 (NEB). Site-directed mutagenesis (Y41A ...
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bioRxiv - Microbiology 2024Quote: ... T4 DNA ligase (NEB) and BsaI-v2 (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... and Phusion High-Fidelity PCR Master Mix (New England Biolabs) were used to set up PCR reactions ...
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bioRxiv - Microbiology 2024Quote: ... samples were split and treated with either 100 U Nuclease P1 (NEB) and 10 U Antarctic phosphatase ...
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bioRxiv - Cancer Biology 2024Quote: ... and Ligase Buffer (New England BioLabs #B0202S). Cell-ligase reaction mixture (40 µL ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM NAD+ (NEB), 4 µg of DNA or RNA oligo ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB) and sent for Sanger sequencing (GENEWIZ).
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bioRxiv - Microbiology 2024Quote: ... These plasmids (Table S6) in combination with the cell-free system PURExpress (NEB) were used to produce bacteriocins in vitro ...
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bioRxiv - Microbiology 2024Quote: ... PCR fragments were amplified with the Q5 polymerase (NEB, Ipswich, MA, USA), following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2024Quote: PCR products of Asaia positive samples were cleaned up using Exonuclease I (Exo I) and Shrimp Alkaline Phosphate (Exo-SAP protocol) according to the protocol of New England Biolabs (NEB, MA, and USA) and sent for sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Deletions of single genes in plasmid pGM255 were performed using the KLD enzyme mix (NEB).
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bioRxiv - Microbiology 2024Quote: ... Using ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs), RNA is denatured and cDNA was synthesized ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids and inserts were assembled using NEBuilder HiFi DNA Assembly (NEB), followed by incubation at 50°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... DNA contamination was removed by DNase I treatment (NEB, USA). The quality and quantity of RNA samples were assessed using NanoDrop One UV-Vis Spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Libraries for Illumina sequencing (average insert size: 700 bp) were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and sequenced using Illumina MiSeq to generate 300 bp paired-end reads ...
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bioRxiv - Molecular Biology 2024Quote: For proximity ligation, the pellet was resuspended into 500 μL of ligation mix (1× T4 DNA ligase buffer, 1× BSA (NEB, B9000S), 5000 CEU T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x, MgCl2 5 mM, 0.5 µl NEB nuclease P1, 0.5 µg benzonase) samples were incubated over night at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin-dNTP at unligated ends was then removed by incubation with 200 U Exonuclease III (NEB, M0206S) in 1× NEB buffer 1 (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼1,000 U of MNase (NEB, M0247S) was added to every cell derived from 100-mL cell culture ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was washed once by 1× T4 DNA ligase buffer (NEB, B0202) and pelleted by centrifugation.
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bioRxiv - Molecular Biology 2024Quote: ... 5000 CEU T4 DNA ligase (NEB, M0202L)) and rotated on a slow rotator for 3 hours or more at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Sequencing of the library was performed using Illumina HiSeq2500 and NextSeq2000 to produce 65-bp single-end reads and 36-bp paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... using Gibson assembly (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Amino acids substitutions in ompT were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Barcodes (Native Barcoding Kit 96 (SQK-NBD109.96)) were ligated using Blunt/TA Ligase Master Mil (NEB). Barcoded libraries were purified using KAPA Pure Beads (Roche ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α (Cat. C2988J, New England Biolabs, Ipswich, MA) and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were processed using the NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB). Barcodes (Native Barcoding Kit 96 (SQK-NBD109.96) ...
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bioRxiv - Microbiology 2024Quote: ... The final adapter (Adapter Mix II Expansion (Oxford Nanopore Technologies, UK) was ligated to the pooled library using the NEBNext Quick Ligation Module (NEB). The prepared DNA library (12 μL ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µL of T4 DNA ligase (New England Biolabs). Following ligation ...
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bioRxiv - Microbiology 2024Quote: ... First-strand cDNA was synthesized using ProtoScript II first strand cDNA synthesis kit (NEB), and quantitative real-time PCR was performed on QuantStudio 3 real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...