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Citations for New England Biolabs :
2451 - 2500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... coli NiCo21(DE3) (NEB BioLabs) was used to express recombinant proteins ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of 10 mM MnCl2 and 1 μl (400 units) of λPP (New England Biolabs, Ipswich, MA). Untreated lysates received 1 μl of H2O in place of λPP ...
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bioRxiv - Immunology 2024Quote: ... and converted into Illumina sequencing libraries with the NEBNext Small RNA Library kit (NEB). Libraries were sequenced on the Illumina NextSeq-550 in the Boston University Microarray and Sequencing Core ...
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bioRxiv - Immunology 2024Quote: ... The sequences containing T7 promoter and sgRNA were PCR-amplified and used as templates to produce sgRNAs through in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). All sgRNAs were purified by lithium chloride precipitation and stored at −80°C.
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bioRxiv - Microbiology 2024Quote: All PCRs needed for cloning were carried out with high fidelity enzymes Q5 polymerase (New England Biolabs) or Accuzyme (Bioline) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing either rpsL-cat or the inducible promoter tetO1 [18] flanked by regions homologous to the DNA up- and downstream of the ggt-promoter were constructed using a commercially available Gibson Assembly Cloning Kit (NEB). Homologous regions were amplified from the genomic DNA of strain PMSS1 using primer pairs FlgK fwd/FlgK rev (rpsL/tetO1 ...
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bioRxiv - Microbiology 2024Quote: ... All three fragments were purified and combined in a Gibson assembly (New England Biolabs [41]). Sequencing revealed a point mutation in the cd3284 open reading frame ...
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bioRxiv - Microbiology 2024Quote: ... Single-construct plasmids expressing A-subunits were constructed via restriction digestion (NdeI and PstI, New England Biolabs) and ligation using pBAD24 (Amp+ ...
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bioRxiv - Microbiology 2024Quote: ... Chimeras were cloned in plasmid pSHU1234 using restriction digestion with NdeI and PstI (New England Biolabs). Plasmids carrying chimeric constructs were used as templates to generate the double-stranded donor DNA used to replace the motA and motB gene on the RP437 chromosome with pomA and potD variants ...
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bioRxiv - Immunology 2024Quote: ... 2μl of endo H (1000U) (New England Biolabs) or 2μl of PBS (mock ...
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bioRxiv - Immunology 2024Quote: ... Gibson assembly was performed using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). 100 ng/μl of pET770 was co-injected along with a body wall muscle co-injection marker (17 ng/μl ...
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bioRxiv - Immunology 2024Quote: ... Deoxynucleotide (dNTP) Solution Mix (N0447L, NEB); Dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2024Quote: ... the drh-1(2CARD) sequence was deleted from pET770 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers LEB003 and LEB004 were used to amplify around the drh-1(2CARD ...
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bioRxiv - Immunology 2024Quote: ... PCR reaction was performed with high-fidelity Q5 polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and PCR products were assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) to generate the final construct pET788 ...
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bioRxiv - Immunology 2024Quote: ... The linearized fragment was subsequently recircularized and transformed into chemically-comptent NEB DH5α cells (New England Biolabs) to generate the resulting plasmid pET786 [vha-6p::mScarlet::unc-54 3’utr] ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were then size-selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Immunology 2024Quote: ... Sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... a ligation reaction was conducted using the T4 DNA ligase (New England Biolabs) with a vector-to-insert ratio of 1:3 ...
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bioRxiv - Microbiology 2024Quote: ... The linearized vector and PCR-amplified sequences were mixed at a 1:2 molar ratio and assembled using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... treated with DNase I (NEB, cat# 50-814-118) and cDNA was prepared with the High Capacity cDNA reverse transcriptase kit (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... and IpgD were constructed using site-directed mutagenesis by PCR amplification using Q5 DNA polymerase (NEB; Table S4).
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bioRxiv - Cell Biology 2024Quote: ... wild-type or mutant VPS35L sequences were amplified using Q5 High-Fidelity 2X Master Mix (NEB, M0492) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the plasmid was also treated with 1.5 µl of quick-CIP (NEB, M0525). Following digestion ...
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bioRxiv - Cell Biology 2024Quote: ... All other site-directed mutagenesis was carried out using Q5 High-Fidelity 2X Master Mix (NEB, M0492) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... These plasmids were transformed into E.coli BL21 DE3 competent cell (New England Biolabs) and plated on agar plates containing Ampicillin or Kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... the isolated DNA was processed to single nucleosides by applying Nucleoside Digestion Mix (NEB, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... GST-IpaH4-3XFlag was cloned into pGEX6P-1 with a 3XFlag peptide followed by the coding sequence for ubiquitin using Gibson Cloning (NEB; Table S4). Sequence-verified constructs were transformed into E ...
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bioRxiv - Microbiology 2024Quote: ... Restriction and cloning enzymes were obtained from New England Biolabs (NEB). Unless stated otherwise ...
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bioRxiv - Cell Biology 2024Quote: ... 1X T4 Ligase (NEB, M0202)) overnight at 4°C in dark humidity chamber followed by proximity ligation assay between biotin and protein of interest.
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were then inverted onto a 50μL drop on parafilm of Ligation Reaction (0.1μM DI-PLA Linker, 1X T4 Ligation Buffer (NEB, B0202), 1mM ATP ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of isolated phage T4 in Pi-Mg buffer was used as a PCR template in the first PCR with the following reaction mix: 0.125 µM each primer (Supplementary Table 2) in 1x High Fidelity Master Mix (NEB) with a total volume of 10 µl ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were amplified using Phusion DNA polymerase (NEB) in reactions containing 10% DMSO and using GC buffer ...
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bioRxiv - Microbiology 2024Quote: Extracted maPgV RNA was subjected to poly(U) tailing using a poly-U polymerase (NEB, Ipswitch, MA) followed by cDNA synthesis with Superscript IV RT (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The SFFV promoter is removed using Q5 site-directed mutagenesis (New England Biolabs). All plasmid constructs were verified using Primordium whole plasmid sequencing (Primordium Labs).
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were capped co-transcriptionally during the T7 RNA polymerase reaction by decreasing GTP to 0.125 mM with addition of 2.5 mM cap analog (G-cap, NEB S1407S; A-cap, NEB S1406S).
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bioRxiv - Cancer Biology 2024Quote: ... were treated with micrococcal nuclease (NEB M0247S) and 0.8 mM CaCl2 for 10 min at 25 °C ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microlitre reaction mixtures were prepared using the Luna® Universal qPCR Master Mix kit (New England Biolabs) as follows ...
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bioRxiv - Microbiology 2024Quote: ... and used in a 1:1 molar ratio Golden Gate assembly reaction with BsaI-HF2 (NEB) and T4 ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and ligated to a puromycin linker primer ([5’Phos]CTCCCGCCCCCCGTCC[SP18][SP18][SP18][SP18][SP18]CC[Puromycin]) using T4 RNA ligase (30 min, 25 °C) (New England Biolabs). First round translation was performed on a 150 µL scale ...
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bioRxiv - Microbiology 2024Quote: ... and T4 ligase (NEB) for 60 cycles of 1.5 min at 37°C followed by 3 min at 16°C ...
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bioRxiv - Microbiology 2024Quote: The PCR product was digested with SfiI and NotI enzymes (NEB) and ligated into a pHEN2 vector modified with a triple c-Myc tag108 which was used to transform electrocompetent TG1 cells (Lucigen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly-A enriched RNA was used to prepare RNA-seq libraries using the NEBNext Ultra RNA Library Prep Kit from Illumina following the manufacturer’s protocol (New England Biolabs). The samples were sequenced in a 2 x 75 bp paired-end configuration using the Illumina Nextseq 500 platform ...
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bioRxiv - Microbiology 2024Quote: ... 25 °C) using a custom methionine(-) Flexible In vitro Translation system composed by PURExpressTM (ΔRF123) Kit (New England Biolabs) solution B ...
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid was blunted by digestion with Quick Blunting™ Kit (NEB, USA) and ligated to re-circularize the plasmid ...
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bioRxiv - Microbiology 2024Quote: ... and T4 ligase (NEB) for 50 cycles of 1.5 min at 37°C followed by 3 min at 16°C ...
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bioRxiv - Microbiology 2024Quote: ... The full-length coding region with introns spliced out of PIGJ was amplified using the Q5 high fidelity polymerase (NEB, M0491) from a CEP cDNA library preparation using primers designed to contain 19 bp homology with the 3’ end of the PIGJ promoter sequence and 25 bp homology with end of the digested pLIC-HA plasmid that contains and provides a 3x HA tag ...
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bioRxiv - Microbiology 2024Quote: ... and T4 ligase (NEB) for 50 cycles of 1.5 min at 37°C followed by 3 min at 16°C ...
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bioRxiv - Microbiology 2024Quote: ... and T4 ligase (NEB) for 50 cycles of 1.5 min at 37°C followed by 3 min at 16°C ...