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Citations for New England Biolabs :
2401 - 2450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged MyoID was generated for this study by cloning cDNA clone SD01662 from Drosophila Genomics Research Center (DGRC) into a pUAST vector using NEBuilder HiFi DNA Assembly Master Mix (NEB # E2621L). Plasmids encoding different Ds-ICD motif deletion constructs with a C-terminal V5-tag were created in this study ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Developmental Biology 2024Quote: ... of 8 or greater were subjected to Poly-A enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB catalog number E7490). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (catalog number E7760L ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were taken up in 520 µl of 1× DpnII buffer (NEB, R0543M) and incubated with 7.5 µl of 20% SDS for 1 hour shaking at 600 r.p.m ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified DNA was inserted into the Hsp68-lacZ vector using the Gibson Assembly kit (E2611S, New England Biolabs). The resulting constructs were verified ...
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bioRxiv - Developmental Biology 2024Quote: ... or SP6 RNA polymerase (NEB, M0207S; sense probe). Embryos used for in situ hybridization were fixed in 4% PFA ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... The rbpms2bsa9329 surrounding genomic region was amplified for 35 cycles with an annealing temperature of 60°C and the mutant allele was digested with MboII (New England Biolabs, R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... The region around the miossa22946 allele was amplified for 35 cycles using 60°C for annealing and the mutant allele was identified by restriction enzyme digestion with DdeI for 1 hour (New England BioLabs, R0175S). The genomic region around the miossa20 allele was amplified for 35 cycles using 57°C for annealing and identified by restriction enzyme digestion of the wild-type allele with BsaWI for 1 hour (New England BioLabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5% SDS) with 10 µg proteinase K (New England Biolabs, P8107S), vortexed briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... 8 injected embryos and 8 control uninjected siblings were assayed by PCR amplification and T7 endonuclease (New England Biolabs, M0302S) digest for mutation analysis41 ...
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bioRxiv - Developmental Biology 2024Quote: ... The genomic region around the miossa20 allele was amplified for 35 cycles using 57°C for annealing and identified by restriction enzyme digestion of the wild-type allele with BsaWI for 1 hour (New England BioLabs). The genomic region surrounding the spo11uc73allele was amplified for 35 cycles with an annealing temperature of 57°C and products were resolved without digestion48 ...
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bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Q5® Site-Directed Mutagenesis (New England BioLabs, Inc.) was used to introduce nonsynonymous mutations into the mlc-4 sequence to generate mlc-4(T15D ...
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bioRxiv - Developmental Biology 2024Quote: ... or 0.2% DMSO (New England BioLabs B0515A) as control ...
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bioRxiv - Developmental Biology 2024Quote: ... and mRNA was transcribed in vitro from the templates using HiScribe T7 ARCA mRNA Kit with Tailing (NEB, E2060) or mMESSAGE mMACHINE SP6 Transcription Kit (Thermo ...
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bioRxiv - Developmental Biology 2024Quote: ... was synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). For RNA-sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR cycles were carried out with Illumina Nextera adapter primers using the NEBNext High Fidelity 2x Master Mix (NEB, Cat# M0541S) using the following PCR program ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR amplified products and cloning vector (pcDNA3.1) were digested to create compatible sites for ligation and transformed into NEB10 beta competent bacteria (NEB, Cat# C3019H). To create plasmids for chick electroporation ...
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bioRxiv - Developmental Biology 2024Quote: ... Sox17 and sox32 deletion and domain switch constructs (Table S1) were generated using pCS2+sox17 and pCS2+myc-sox32 by PCR amplification using Q5 polymerase (NEB) using described primers (Table S2 ...
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bioRxiv - Developmental Biology 2024Quote: ... except for Sox17Δ7aa + Sox32 25aa and myc-Sox32ΔHMG + Sox17 HMG where NEBuilder HiFi DNA Assembly (NEB), with Sox17Δ7aa + Sox32 25aa as a gBlock (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mM ribonucleoside vanadyl complex (New England Biolabs), and 2 mg/ml bovine serum albumin (Jackson ImmunoResearch) ...
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bioRxiv - Developmental Biology 2024Quote: ... ∼300 ngs of genomic DNA were digested with 100U of MspI (New England Biolabs), and cleaned up using QIAquick PCR purification columns (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was treated with DNAse I (NEB, M0303S) according to manufacturer’s protocol and used for reverse transcription with iScript cDNA synthesis kit (Bio-rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Total cDNA for RT-qPCR was generated from 1.5 µg total RNA using a random primer mix and M-MuLV reverse transcriptase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of plasmid was incubated with 8 U M.SssI (NEB) at 37°C for 4 h for in vitro DNA methylation ...
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bioRxiv - Genetics 2024Quote: ... Streptavidin magnetic beads (New England Biolabs) were washed 3 times with B&W buffer I (100 mM NaCl ...
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bioRxiv - Genetics 2024Quote: ... and 0.1U Shrimp Alkaline Phosphatase (NEB).
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bioRxiv - Genetics 2024Quote: ... DNA digested overnight with XhoI was further digested for ∼5 h with HF-BamHI or NgoMIV (New England Biolabs). Digested DNA was precipitated using standard ethanol/ sodium acetate precipitation.
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bioRxiv - Genetics 2024Quote: ... Genome PCR products which were confirmed as a single band in AGE were subjected to direct Sanger sequencing after decomposing remaining nucleotides and primers by the treatment with 2U Exonuclease I (NEB) and 0.1U Shrimp Alkaline Phosphatase (NEB).
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bioRxiv - Genetics 2024Quote: ... the slides were treated with 3 U/µl exonuclease III (New England Biolabs) in 1x NEB cutsmart buffer and incubated at 37°C for 15 min to digest nicked BrdU-positive strands ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA libraries were amplified using 1X NEBNext Ultra II Q5 Master Mix (New England Biolabs M0544), 1X EvaGreen dye (Biotium 31000) ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL rAlbumin (New England Biolabs B9200S), and 1 U/µL Phi29 DNA polymerase (Thermo Fisher Scientific EP0091) ...
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bioRxiv - Genomics 2024Quote: ... and 100 µg/mL rAlbumin (New England Biolabs B9200S) in 1X PBS ...
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bioRxiv - Genomics 2024Quote: ... 250 µM dNTPs (New England Biolabs N0447L), 200 µg/mL rAlbumin (New England Biolabs B9200S) ...
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bioRxiv - Genomics 2024Quote: ... 50 nM dNTPs (New England Biolabs N0447L), 0.1 µM each padlock probe ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL rAlbumin (New England Biolabs B9200S), 0.4 U/µL RNase H (Enzymatics Y9220L) ...
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bioRxiv - Genomics 2024Quote: ... 250 µM dNTPs (New England Biolabs N0447L), 1 µM each biotinylated reverse transcription primer (Integrated DNA Technologies) ...
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bioRxiv - Genetics 2024Quote: ... no more than 5µg total RNA was treated with DNase I (NEB) for 30 minutes at 37°C then purified using RNA Clean & Concentrator -5 Kit ...
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bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
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bioRxiv - Genomics 2024Quote: ... and random primer 6 (NEB). The NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module was subsequently used to convert single-stranded cDNA to double-stranded cDNA ...
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bioRxiv - Genomics 2024Quote: ... Reverse transcription cDNA synthesis was performed using NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB, E6421). Samples were barcoded and the library pool was prepared according to the guidelines laid out in the Iso-Seq protocol version 02 (PacBio ...
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bioRxiv - Genetics 2024Quote: ... coli 10β cells (New England BioLabs, C3019I). Following heat-shock ...
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bioRxiv - Genetics 2024Quote: ... and the recommended protocol from NEB was followed ...
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bioRxiv - Genetics 2024Quote: ... cDNA synthesis was performed using the ProtoScript® First Strand cDNA Synthesis Kit (NEB). One microliter of oligo d(T)23VN primer (50 μM ...
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bioRxiv - Genetics 2024Quote: ... PCR amplification was performed with Phusion or Q5 High Fidelity DNA Polymerase (New England Biolabs). Primer sequences are provided in Supplementary Table 4.
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bioRxiv - Genetics 2024Quote: ... One microliter of oligo d(T)23VN primer (50 μM) (NEB) was added to 200 ng/µL of RNA (170 ng worm RNA + 35 ng yeast RNA ...