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Citations for New England Biolabs :
2351 - 2400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared with 5ng of CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (Cat # E7645S, NEB) following manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... Exonuclease I treatment (NEB M0293 L) was used to remove excess primers ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Immunology 2024Quote: ... The third PCR was performed with the Phusion hot Start (NEB) to add the Illumina adapter and index ...
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bioRxiv - Immunology 2024Quote: ... A second amplification was performed with the Phusion Hot Start DNA Polymerase (NEB). A forward primer ...
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bioRxiv - Immunology 2024Quote: ... cDNA for 3’ RACE was generated using RNA from IFN-β treated A549 cells using the Template Switch RT Enzyme Mix (NEB, #M0466) and anchored primers ...
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bioRxiv - Immunology 2024Quote: ... IVT DI was in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs) followed by DNAse I clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Immunology 2024Quote: ... and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... 1% penicillin/streptomycin (Nordic Biolabs), referred to complete medium (CM) ...
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bioRxiv - Immunology 2024Quote: VR therapy vectors and the CAR therapy vector were first designed through Gibson Assembly molecular cloning (HiFi DNA Assembly Cloning Kit, NEB) using the backbone pSLCAR-CD19-BBz (Addgene) ...
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bioRxiv - Microbiology 2024Quote: ... Linker was ligated into purified linearized vector using T4 ligase (NEB) at 4°C overnight followed by transformation into DH5α E ...
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bioRxiv - Microbiology 2024Quote: ... Version: NBE_9134_v112_revE_01Dec2021) using a NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367), a NEBNext Quick Ligation Reaction Buffer (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and a NEBNext Companion Module for Oxford Nanopore Technologies (New England Biolabs, cat. no. E7180S). Sequencing was performed on the NanoPore MINIon MkB1 instrument using the R10.4 version of reagents (cat ...
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bioRxiv - Microbiology 2024Quote: ... the total RNA samples were fragmented using ultrasound (4 pulses of 30 sec at 4°C) followed by a treatment with T4 Polynucleotide Kinase (New England Biolabs). The RNA samples were then split into two halves and one half was subjected to Terminator Exonuclease treatment (+TEX) ...
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bioRxiv - Microbiology 2024Quote: ... Libraries for Illumina sequencing were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Examples of plasmid DNA with similar profiles were additionally differentiated by digestion with restriction enzymes BamHI and HindIII (NEB, USA). The digestion was performed according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2024Quote: ... a NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. no. B6058) and a NEBNext Companion Module for Oxford Nanopore Technologies (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... 2 kb fragments upstream and downstream of SrcF were PCR amplified with Q5 High Fidelity DNA Polymerase (New England Biolabs) and cloned in PCR amplified pEx-deletion-ermG via DNA Gibson assembly (HiFi DNA Assembly Master Mix ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs), multiplexed ...
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bioRxiv - Genomics 2024Quote: Between 2 and 4 million cells were harvested by centrifugation and high molecular weight (HMW) genomic DNA was extracted using the Monarch High Molecular Weight DNA Extraction kit (NEB) and agitation speeds of 1,500 rpm.
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bioRxiv - Genomics 2024Quote: ... and the NEBNext UltraII End Repair/dA-Tailing Module (New England BioLabs) and followed by the sequencing adaptors ligation ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with the manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... cells were washed three times with PBS-T and incubated with a gap-fill Phusion mix (1x Ampligase Buffer, 0.4 U/mL RNase H, 100 nM padlock probe oPD_CROPseq_iT7, 0.0125 U/mL NEB Phusion polymerase ...
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bioRxiv - Genomics 2024Quote: ... and individually dual-barcoded using a New England Biolabs Ultra II Directional kit (NEB #E7765). Individually barcoded samples were pooled and sequenced paired end and 150 bp in length on two lanes of a Novaseq at the University of New Hampshire Hubbard Center of Genome Studies.
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bioRxiv - Genomics 2024Quote: ... Genomically integrated guide sequences were amplified using NEBNext PCR polymerase (NEB) and staggered guide-specific primers (Supplementary Table 1 ...
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bioRxiv - Genomics 2024Quote: ... The high-quality HMW DNA molecules were treated with Nb.BssS1 (New England BioLabs, Ipswich, MA) to generate single-strand breaks with sequence-specific motifs (GCTCTTC) ...
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bioRxiv - Genomics 2024Quote: ... 4.0 μg of the pool DNA was repaired and end-repaired using NEBNext FFPE DNA Repair Mix (New England BioLabs, Ipswich, MA, USA) and the NEBNext UltraII End Repair/dA-Tailing Module (New England BioLabs ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) and the supplied protocol for yeast ...
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bioRxiv - Immunology 2024Quote: ... and ligated by NEBuilder HiFi DNA Assembly (NEB) per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The reaction consisted of 50 µL of NEBNext High Fidelity 2X PCR Master Mix (NEB), 4 µg of genomic DNA ...
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bioRxiv - Immunology 2024Quote: ... by NEBuilder HiFi DNA Assembly (NEB) using the same approach as BaEVRless plasmid ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions with the following specifications and modifications ...
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bioRxiv - Immunology 2024Quote: ... top and bottom strand oligos (1 µl of 100 µM each) were phosphorylated with T4 PNK (New England Biolabs) for 30 minutes at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... NEBNext adaptor for Illumina from NEBNext Multiplex Oligos for Illumina (New England Biolabs) was diluted 25-fold in TBS buffer ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Second-strand synthesis was performed by addition of 2 µl Klenow-Fragment DNA Polymerase (New England Biolabs) and incubation for 15 minutes at 30°C ...
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bioRxiv - Immunology 2024Quote: ... including DNAseI (RNase free, New England Biolabs) digestion to remove genomic DNA ...
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bioRxiv - Immunology 2024Quote: Reverse-transcription on total RNA (100 to 500 ng) was performed with random hexamer/oligo-dT-Primer containing LunaScript RT Supermix (New England Biolabs) following the manufactureŕs instructions ...
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bioRxiv - Immunology 2024Quote: ... Q5 NEBNext® Ultra™ II Q5® Master Mix (New England Biolabs) for 10-14 cycles ...
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bioRxiv - Immunology 2024Quote: ... 0.15 µl Murine RNase Inhibitor (New England Biolabs) and 0.2 µl Superscript III enzyme (Thermofisher ...
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bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Two microliters were subsequently used in real-time quantitative PCR reactions containing SYBR-green based Luna universal dye qPCR mix (New England Biolabs) and gene specific PCR primers ...
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bioRxiv - Immunology 2024Quote: ... Then the poly(A) RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB) under 86°C for 7 min ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA was amplified in two rounds using NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs). In PCR 1 ...
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bioRxiv - Immunology 2024Quote: ... The sgRNA cassette was amplified with NEBNext Ultra II Q5 HiFi polymerase (New England Biolabs) with indexed primers using the following steps with 5 μg of gDNA input per 50 μL reaction ...
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bioRxiv - Microbiology 2024Quote: ... and Ph.D.-C7 peptide library (New England Biolabs, Ipswich, MA, USA) for the identification of AbOmpA binding phage ...
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bioRxiv - Immunology 2024Quote: ... The unpurified DNA product was then subjected to in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), incubating at 37 °C for 16 h ...
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bioRxiv - Immunology 2024Quote: ... The NEBNextII kit (New England Biolabs) was used to prepare the sequencing library ...
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bioRxiv - Immunology 2024Quote: ... and sgRNA scaffold were amplified using Phusion polymerase (New England Biolabs). The unpurified DNA product was then subjected to in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... All PCR products were amplified using Q5-High Fidelity DNA polymerase according to the manufacturer (New England Biolabs).