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Citations for New England Biolabs :
2301 - 2350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... coli DNA ligase (New England Biolabs). After the second strand synthesis the cDNA of all single cells of a plate were pooled ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using the NEBNext rRNA Depletion kit (New England Biolabs), followed by the conversion of the remaining RNA into an Illumina sequencing library using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2uL 10uM uniquely barcoded i7 primer and 25uL PCR master mix (NEB Phusion® High-Fidelity PCR Master Mix with HF Buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... and the Cap structure analog (New England Biolabs). Pink Flamindo mRNA was synthesized with the mMESSAGE mMACHINE T3 Transcription kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were prepared with NEBNext Ultra I Directional RNA Library Prep with rRNA Depletion (New England Biolabs, Inc., Ipswich, MA). Reads were aligned (Novoalign ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by the conversion of the remaining RNA into an Illumina sequencing library using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs). Following library preparation ...
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bioRxiv - Developmental Biology 2024Quote: ... The ribosome profiling library was prepared using NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... and ProtoScript® II reverse transcriptase (M0368L, New England Biolabs). RT-qPCR was performed on a LightCycler 480 instrument (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... the RNA underwent quantification and quality assessment before library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs, US). All steps were performed as recommended in user manual E7760 (Version 1.0_02-2017 ...
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bioRxiv - Cell Biology 2024Quote: ... The purified cDNA was added to a mixture of 10× terminal transferase buffer (NEB, no. M0315S), 2.5 mM CoCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 U/μL Taq DNA polymerase (NEB) for incubation at 25°C for 60 min and heating at 68°C for 30 s and then at 75°C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... RNF43 mutants were generated by PCR-subcloning using Q5 High-Fidelity 2× Master Mix (NEB). Domain swapped constructs were generated by in-fusion cloning (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 U/μL terminal transferase (NEB, no. M0315S). After incubation at 37°C for 30 min and termination at 70°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... coli (New England Biolabs C2527H) underwent transformation with wild-type α-syn/pET21a ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA resistant mCherry-ALG-2 was created by mutagenesis (primers sequences 5’-ATTTCGATGTTTGACCGTGAGAAC-3’, 5’-AATGGACCTGACAGTCACTGGATTAAA-3’) using a Q5 Site-Directed Mutagenesis Kit (E0554S, NEB). The plasmid encoding IST1-GFP is as described (63 ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmid encoding TMEM192-GCAMP7 was constructed by cloning TMEM192 and GCAMP7 sequence into pcDNA 3.1/Zeo (+) using Gibson Assembly (E2611, NEB). The plasmids encoding GCAMP7-TRPML1 ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids in which multiple cdk5 phosphorylation sites in the DCX C-terminus were replaced with non-phosphorylatable or phosphomimetic residues were cloned by Gibson assembly (New England Biolabs) of a synthetic DNA fragment carrying these changes (synthesized by Twist Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... newly deposited CENP-A was labelled with 3 µM SNAP-Cell® 647-siR (S910102S, NEB) for 30 mins before washing excess with media and allowing to grow for a further 30 mins ...
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bioRxiv - Cell Biology 2024Quote: ... and was assembled with the PCR fragments in NEBuilder® HiFi DNA assembly reaction (NEB) and transformed into DH5α E ...
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bioRxiv - Immunology 2024Quote: ... the plasmids were linearized by digestion with SpeI (New England BioLabs), followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Immunology 2024Quote: ... Resulting mRNA products were purified using Monarch RNA Cleanup Kit (New England BioLabs), visualized on 1% TBE gel ...
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bioRxiv - Immunology 2024Quote: ... pUC57-mini plasmids harboring the WT or mutated coding sequence downstream of T7 promoter were first transcribed into mRNA using the HiScribe T7 ARCA mRNA Kit (New England BioLabs) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: N-linked glycans were enzymatically released from purified mouse THP using PNGase-F kit (catalog no P0709S, New England Biolabs). N-glycans were then purified from the reaction mixture containing denaturing buffer and de-N-glycosylated proteins by solid phase extraction method using Sep-Pak C18 (1 cc Vac-cartridges ...
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bioRxiv - Immunology 2024Quote: ... were prepared and sequenced at Azenta (South Plainfield, NJ) using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The sequencing libraries were validated on the Agilent TapeStation ...
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bioRxiv - Immunology 2024Quote: ... Gibson assembly was performed using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). 100 ng/μl of pET770 was co-injected along with a body wall muscle co-injection marker (17 ng/μl ...
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bioRxiv - Immunology 2024Quote: ... Deoxynucleotide (dNTP) Solution Mix (N0447L, NEB); Dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2024Quote: ... the drh-1(2CARD) sequence was deleted from pET770 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers LEB003 and LEB004 were used to amplify around the drh-1(2CARD ...
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bioRxiv - Immunology 2024Quote: ... PCR reaction was performed with high-fidelity Q5 polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and PCR products were assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) to generate the final construct pET788 ...
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bioRxiv - Immunology 2024Quote: ... The linearized fragment was subsequently recircularized and transformed into chemically-comptent NEB DH5α cells (New England Biolabs) to generate the resulting plasmid pET786 [vha-6p::mScarlet::unc-54 3’utr] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... NEBNext® Multiplex Oligos for Illumina® (New England BioLabs, Ipswich, Massachusetts, USA) using DNA NEBNext® UltraTM II Library Prep Kits at half the recommended volume ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was extracted with the RNA Miniprep Kit (New England Biolabs, #T2010) according to the manufacturer’s instructions and used for standard strand-specific Illumina library prep ...
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bioRxiv - Genomics 2024Quote: ... After adding 10 µL of 20 mg/µL proteinase K (New England Biolabs), proteins were degraded for 30 minutes at 55°C followed by an overnight incubation at 68°C with shaking to reverse crosslinks ...
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bioRxiv - Genomics 2024Quote: ... Q5 Site-directed mutagenesis kit (#E0554S, NEB) was used to produce intronless ...
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bioRxiv - Genomics 2024Quote: ... For molecular cloning applications Q5 High-Fidelity DNA polymerase (NEB) was used ...
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bioRxiv - Genomics 2024Quote: ... dATP (3x 1.5 µL of 10 mM solutions) and 8 µL of 5 U/µl Klenow fragment of DNA polymerase I (New England Biolabs) and a 30-minute incubation at 37°C with rotation ...
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bioRxiv - Genomics 2024Quote: ... teleta were assayed by Enzymatic-Methyl seq (EM-seq) (NEB #E7120) according to the manufacturer-provided protocol ...
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bioRxiv - Genomics 2024Quote: RT-PCR was conducted using Quick-Load Taq 2X Master Mix (NEB). For molecular cloning applications Q5 High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 120 µL 10x T4 DNA ligase buffer and 5 µL of 400 U/µL T4 DNA ligase (New England Biolabs) was added ...
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bioRxiv - Genomics 2024Quote: ... 25 µL 10x CutSmart buffer and 10 µl 10 U/µL MseI restriction enzyme (New England Biolabs) were added ...
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bioRxiv - Genetics 2024Quote: ... in reactions containing 1X ThermoPol buffer (12.5mM tricine, 42.5mM KCl, 1.5mM MgCl2, 6% dextran; New England BioLabs, Ipswich, MA), 0.32 mM dNTP mix ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2.5 μl 25μl p7 custom primer (Buenrostro et al. 2013) and 25 μl NEBNext Ultra II Q5 2x Master Mix (NEB) and a PCR with 10 cycles was conducted as stated in the Omni-ATAC protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and gel excision of DNA between 150 bp and 1,500 bp was performed using the Monarch DNA Gel Excision Kit (NEB). Concentrations of the purified libraries were measured using PicoGreen (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reaction mix was purified with Monarch® PCR & DNA Cleanup Kit (NEB, Ipswich, MA). Next ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 100 ng of DNA was cleaved with PstI HF and MseI restrictases in CutSmart® buffer (New England Biolabs; 3 hours at 37°C). P1 and P2 barcoded adapters corresponding to the restriction site of the enzymes were ligated immediately afterwards with T4 ligase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was digested with the restriction enzymes MseI and EcoRI (New England Biolabs, Ipswich, MA, USA). Genomic libraries were pooled at equimolar ratios and size selected for fragments between 200-250 base pairs (bp ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pET09 vector was opened with BsaI enzyme and dephosphorylated with rSAP enzyme (NEB, Ipswich, MA), PCR amplicons of furnished FPs were digested with BsmBI enzymes and following clean-up all three elements were ligated with T4 DNA ligase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used PCR with long protruding primers to add GGS sequences and BsaI sites directly to the genes of mTurquiose2 and mVenus and assembled them with the pET09 via NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2) (NEB, Ipswich, MA). Control proteins were PCR amplified from either E.coli DH5 αgenome or in-house generated constructs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplicons of furnished FPs were digested with BsmBI enzymes and following clean-up all three elements were ligated with T4 DNA ligase (NEB, Ipswich, MA).