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Citations for New England Biolabs :
2201 - 2250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 2 μl of Antarctic Phosphatase Enzyme (NEB), and 2 μl of water ...
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bioRxiv - Genomics 2024Quote: ... 4 μl of Inorganic Pyrophosphatase (NEB) and 2 μl of Phi29 DNA Polymerase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... and then 2 μl of T7 Endonuclease (NEB) was added ...
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bioRxiv - Genomics 2024Quote: ... any residual linear DNA is digested using Exonuclease V (NEB). At this point ...
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bioRxiv - Genomics 2024Quote: ... and 2 μl of Phi29 DNA Polymerase (NEB). The reaction is incubated for 8 hours at 30°C.
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bioRxiv - Genomics 2024Quote: ... Large (Klenow) fragment without exonuclease activity (exo-) (NEB) in combination with dATP or dGTP ...
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bioRxiv - Genomics 2024Quote: ... 5 μl of CutSmart 10x (NEB), 2 μl of BSA (NEB) ...
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bioRxiv - Genomics 2024Quote: Methylation enrichment of cfDNA was performed using the EpiMark Methylated DNA Enrichment Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 5 μM each) from IDT were annealed in 1x New England Biolabs (NEB) buffer 2 (20 μl in total) ...
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bioRxiv - Genomics 2024Quote: The reaction is supplemented with 2 μl Exonuclease V (NEB) and 5 μl of ATP 10 mM ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries for regular cfDNA were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... the cfDNA can be de-phosphorylated by adding 6 μl of Antarctic Phosphatase buffer 10x (NEB), 2 μl of Antarctic Phosphatase Enzyme (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 2.5 μl of DTT and 2 μl of Blunting Enzyme (NEB) in a total reaction volume of 50 μl ...
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bioRxiv - Genomics 2024Quote: ... the reaction is treated with 2 μl of Proteinase K (NEB). The digestion is carried on at 37°C for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... dTTP (NEB), and 10 U Klenow exo− (M0212L ...
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bioRxiv - Genomics 2024Quote: For a typical blunting reaction 5-50 ng of cfDNA are mixed with 5 μl of CutSmart 10x (NEB), 5 μl of dNTPs 1mM each ...
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bioRxiv - Genomics 2024Quote: ... 2.0 U of Proteinase K (New England Biolabs) was introduced to the reaction mixture and incubated at 50°C for 1 hour to stop the oxidation process ...
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bioRxiv - Genomics 2024Quote: ... IVT reactions were set up in 30 μl total volume with the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) with 2 μl of each NTP ...
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bioRxiv - Genomics 2024Quote: ... 2 μl of BSA (NEB), 15 μl of dNTPs 10 mM ...
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bioRxiv - Genomics 2024Quote: ... were amplified by PCR and cloned at the ClaI site in the Rosa26-targeting vector pEN111 by Gibson assembly (New England Biolabs). The resulting vectors obtained (pEN111-TRE3G-LKF/AKF-IRES-ZsGreen1-rtta3G_Rosa26-donor ...
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: ... A combination of 48 i7 oligos (NEBNext Multiplex Oligos for Illumina, NEB) and 24 i5 oligos (Microsynth ...
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Genomics 2024Quote: ... in the presence of 1% VRC (Vanadyl Ribonucleoside Complex; NEB, S1402S). Coverslips were then rinsed 3 times in 70% EtOH and stored at -20C ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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bioRxiv - Genomics 2024Quote: ... RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog #: E7760, New England Biolabs Inc.), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: The custom double stranded adapter containing primer-binding site was ligated by mixing 10 μl of NEB TA/Blunt Ligase Master Mix (cat #M0367, New England Biolabs MA, USA), 2 μl of duplex adapter (1 μM ...
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bioRxiv - Genomics 2024Quote: ... was mixed with 1.75 μl of Ultra II End-prep Reaction Buffer and 1.5 μl of Ultra II End-prep Enzyme Mix (cat # E7546, New England Biolabs MA, USA) and incubated at 20°C for 5 min ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 0.2 U/µL RNase Inhibitor (NEB cat. #M0314L). A gentleMACS Octo Dissociator (Miltenyi Biotec cat ...
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bioRxiv - Genomics 2024Quote: ... Incompletely formed SMRTbell templates were removed by digestion with a combination of exonuclease III and exonuclease VII (NEB, MA). The qualification and quantification of the SMRTbell libraries were made on a Qubit fluorimeter (Invitrogen ...
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bioRxiv - Genomics 2024Quote: The gRNA libraries were amplified from each gDNA sample across 100 μL PCR reactions using Q5 hot start polymerase (NEB, M0493) with 1 μg of gDNA per reaction ...
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bioRxiv - Genomics 2024Quote: ... Tagmented DNA was then amplified with Phusion high-fidelity PCR master mix (NEB) using 14 PCR cycles ...
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bioRxiv - Genomics 2024Quote: ... using the Monarch DNA purification kit (New England Biolabs, Inc., Rowley, MA, USA) to extract genomic DNA from the original frozen whole blood samples ...
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bioRxiv - Genomics 2024Quote: ... coli Lemo21 (DE3) (New England Biolabs), which was grown at 30°C in lysogeny broth with 30 mg L-1 chloramphenicol and 50 mg L-1 kanamycin until an optical density (600 nm ...
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bioRxiv - Genomics 2024Quote: ... PAP enzyme and ATP (NEB, M0276L) were also added to the cell mix to facilitate in vitro polyadenylation ...
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bioRxiv - Genomics 2024Quote: ... 20 µL of 2% BSA (New England Biolabs, catalog no. B9000S), and 1.86 mL of nuclease-free water ...
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bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: In vitro transcription was carried out using HiScribe® SP6 RNA Synthesis Kit (NEB, E2070S) in a 25 μl reaction containing 1 x SP6 Reaction Buffer ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Genomics 2024Quote: ... The library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina-compatible y-adaptor ...
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bioRxiv - Genomics 2024Quote: DNA templates for in vitro transcription of λRNA were generated from a defined region of λDNA (NEB, N3011S) through PCR amplification ...
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bioRxiv - Genomics 2024Quote: ... Poly(A) mRNA was selected using Oligo d(T)25 Magnetic Beads (NEB, S1419S) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and 13% second strand synthesis enzyme mix (NEB) was added ...
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bioRxiv - Genomics 2024Quote: ... along with a 4.8uL 3:2 master mix of T4 ligase buffer:T4 ligase (New England Biosciences, NEB) and 9.4uL of nuclei buffer with BSA (NBB ...
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bioRxiv - Genomics 2024Quote: ... 20uL 2X NEBnext PCR master mix (NEB) was added ...
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bioRxiv - Genomics 2024Quote: ... Resulting dsDNA was purified using a 1X SPRIbead clean-up within the 96-well plate, and the resulting product was subjected to USER digestion (80% ddH2O, 10% 10X rCutsmart, 10% USER enzyme (NEB)) ...
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bioRxiv - Immunology 2024Quote: ... Poly(A) mRNA Magnetic Isolation Module (New England BioLabs) was used for cDNA library preparation according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using NEBNext Ultra II Library Prep Kit for Illumina (NEB), size selected (150-500 bp) ...
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bioRxiv - Immunology 2024Quote: ... DNA library construction was performed using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645L). The libraries were sequenced on Illumina NovaSeq 6000 system (50 bp paired-end ...
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bioRxiv - Genomics 2024Quote: ... and 1X BsmBI-v2 Golden Gate Enzyme Mix (New England Biolabs E1602S) with the thermal cycling protocol ...
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bioRxiv - Genomics 2024Quote: ... dephosphorylated with rSAP (New England Biolabs M0371L), and gel purified with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506) ...