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Citations for New England Biolabs :
2151 - 2200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The Ni-elution was collected directly on a pre-equilibrated amylose column (amylose high flow resin, New England Biolabs, Ipswich, Massachusetts). Amylose column was washed with 5 column volume cold wash buffer before fractionated elution in a buffer containing 25 mM Hepes pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcription was performed by the ProtoScript® II reverse transcriptase (NEB) using random primers ...
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Developmental Biology 2024Quote: ... Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs) or by Qubit (Thermo) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA and library preparation were performed using NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina (New England Biolabs). Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 600-800 ng of genomic DNA per-sample (depending on the library, see Table S2, Table S3) was digested using the SbfI-HF enzyme (New England Biolabs). Custom P1 adapters containing unique 7-bp barcodes (Hohenlohe ...
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bioRxiv - Genomics 2024Quote: Experiments in T-47D and LNCaP cells were conducted using restriction enzyme MboI (New England Biolabs). Crosslinked chromatin was sonicated using Covaris E220 with the settings of fill level=10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Custom P1 adapters containing unique 7-bp barcodes (Hohenlohe, Bassham, Currey, & Cresko, 2012) were ligated to each individual sample with T4 ligase (New England Biolabs). The DNA from all uniquely barcoded individuals in a library was pooled at equimolar concentration ...
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bioRxiv - Developmental Biology 2024Quote: ... a mix containing 18µM sgRNA and 12µM Cas9 protein (EnGen™ Spy Cas9-NLS S. pyogenes, NEB Cat# M0646T) was incubated for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Cas9 Nuclease Reaction Buffer (NEB Cat# B0386A) was added to obtain a final 45µM sgRNA stock solution ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 200 ng DNA using the NEB Enzymatic Methyl-Seq kit (NEB #E7120) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We digested genomic DNA with restriction enzymes EcoRI and MseI (New England Biolabs) and ligated Illumina primer and adapter sequences to the cut sites with an individual 8-nucleotide barcode on the EcoRI side ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 U of Q5 HotStart Taq polymerase (New England Biolabs) in 1 X Q5 buffer ...
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bioRxiv - Physiology 2024Quote: ... mRNA Magnetic Isolation Module was followed by library preparation using the NEBNext Ultra II RNA Directional Library Prep Kit for Illumina (New England BioLabs). Single read sequencing took place on a NextSeq 2000 System (Illumina) ...
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bioRxiv - Plant Biology 2024Quote: ... Both the synthesized genes and PCR amplified genes were then inserted into a doubly digested BamHI/XhoI pET28b(+) through Gibson assembly using the 2X Gibson Assembly Master Mix (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Q5 2X Hotstart master mix (New England Biolabs, Ipswich, MA, USA) was used for cloning PCRs ...
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bioRxiv - Developmental Biology 2024Quote: ... the rbm8a wild type allele was genotyped by restriction digest of the PCR product with Xmn1 (R0194S, NEB) and the rbm8aΔ3 allele by Hinf1 (R0155S ...
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bioRxiv - Synthetic Biology 2024Quote: ... while Gibson assembly reactions used the NEBuilder(R) HiFi DNA Assembly Cloning Kit (NEB). Transformed bacteria were grown for 24h at 32°C ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction digest into Tol2kit vector 396 (pCS2FA:Tol2)161 cut with BamHI and XbaI (R0145S, NEB).
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was purified using the MonarchR RNA Cleanup Kit (NEB). The integrity of the purified RNA was determined by running RNA in a 1% agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... Insert was amplified by PCR and digested with BbsI (NEB) to produce sticky ends compatible with plasmid ligation.
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bioRxiv - Developmental Biology 2024Quote: ... NheI (R3131S, NEB) restriction digest into Tol2kit vector 396 (pCS2FA:Tol2)161 cut with BamHI and XbaI (R0145S ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Developmental Biology 2024Quote: ... A primer extension reaction was performed using Phusion DNA polymerase (M0530L, NEB) followed by QIAquick purification (28106 ...
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bioRxiv - Synthetic Biology 2024Quote: Desired sequences were amplified using two subsequent PCR reactions using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB). All extension steps were carried out in a 15s timeframe and the first PCR round consisted of 10 cycles ...
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bioRxiv - Synthetic Biology 2024Quote: All cloning was performed using the Gibson assembly (NEBuilder HiFi DNA Assembly, New England Biolabs). PCR reactions were performed utilizing the Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2024Quote: All protein expression constructs (Supplementary Table S1) were cloned using Gibson assembly (NEB, where double-stranded DNA fragments are either ordered from IDT as gBlocks or PCR amplified from existing constructs with at least 25-bp overlap in sequence ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed with DNaseI reaction buffer (New England Biolabs, Ipswitch, MA), resuspended in DNaseI buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Mutagenesis of NGR1p::NGR1-GFP was performed using Gibson assembly (NEB HiFi DNA assembly E2621L), overlapping compatible cohesive ends were modified ...
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bioRxiv - Synthetic Biology 2024Quote: ... SapI and PaqCI (New England Biolabs, Ipswitch, MA) were used in different levels of modular assembly ...
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bioRxiv - Plant Biology 2024Quote: ... A 517bp product was amplified using the primers specified in Table S4 and S5 and digested with BsaI-HFv2 (NEB) according to the manufacturer’s guidelines before gel electrophoresis of the resulting product on 3% agarose gel ...
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bioRxiv - Plant Biology 2024Quote: ... The NEB Next® DNA Library Prep Master Mix set for Illumina kit (NEB #E6040S) was implemented to prepare the DAP-Seq gDNA library ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Genomics 2024Quote: ... HiChIP in Ishikawa cells was conducted using restriction enzyme DpnII (New England Biolabs). Crosslinked chromatin was sonicated using an EpiShear probe-in sonicator (Active Motif ...
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bioRxiv - Genomics 2024Quote: ... The 5′ adapter was ligated with T4 RNA ligase (NEB), followed by a third biotinylated RNA enrichment ...
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bioRxiv - Genomics 2024Quote: ... in multiple ligation reactions (NEB T4 ligase). Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB ...
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bioRxiv - Genomics 2024Quote: ... and digested with NheI-HF and SphI-HF (NEB). Adding mBCs to the reporter gene allows us to test larger libraries by amplifying sublibraries with different primer sets and cloning each sublibrary in parallel with a unique mBC ...
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genomics 2024Quote: ... and digested using DpnII (NEB) overnight at 37°C ...
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bioRxiv - Genomics 2024Quote: ... then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase). Ligation products were transformed into electrocompetent cells and grown in liquid culture from which we prepared plasmid DNA.
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bioRxiv - Genomics 2024Quote: ... followed by 5′ cap repair with RppH (NEB) and 5′ hydroxyl repair with PNK (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.01% Molecular Grade BSA (New England Biolabs), 146 mM NaCl (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep kit with poly(A) mRNA isolation was used to construct RNA-seq libraries according to the manufacturer’s instructions (NEB). Sequencing reads were aligned to hg19 build of the human genome using HISAT2 (Kim et al. ...
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bioRxiv - Genomics 2024Quote: ... dCas9-VP16(10x) plasmid was linearized with restriction enzyme AflII (New England Biolabs R0520S). Successful integration of the dCas9-VP16(10x ...
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bioRxiv - Genomics 2024Quote: ... followed by 3′ adapter ligation using T4 RNA ligase (NEB). Biotinylated RNAs were enriched for a second time ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genomics 2024Quote: ... Plasmid pools were digested with SphI-HF and SpeI-HF and treated with Antarctic phosphatase or Quick CIP (NEB), then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase) ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBNext Small RNA Library Prep kit (NEB), RNA oligonucleotides (Horizon Discoveries) ...