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Citations for New England Biolabs :
2101 - 2150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were fixed with 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized with ice-cold permeabilization buffer (1X-PBS, 0.5% Triton X-100, 2 mM vanadyl-ribonucleoside complex (New England Biolabs)) for 4 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... Genotyping was performed with Quick-Load Taq 2X Master Mix (M0271L, BioLabs), on 2% agarose gel for the PCR bands ...
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bioRxiv - Developmental Biology 2024Quote: Sequencing libraries were prepared using a commercial kit (NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, New England Biolabs, Ipswich, USA), and cDNA of three replicates per strain was sequenced as 150 bp (100 bp for Mpyr_CVe13f ...
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bioRxiv - Developmental Biology 2024Quote: ... Poly(A)-tailed RNAs were enriched and purified using oligo-dT beads from the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). These samples then underwent fragmentation ...
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bioRxiv - Developmental Biology 2024Quote: ... and PCR amplification to prepare cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, USA). Library preparation and next-generation sequencing were outsourced to Rhelixa Inc ...
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bioRxiv - Developmental Biology 2024Quote: ... The above two sets of PCR products were gel purified and cloned in a pUAST-attB vector digested with EcoRI using NEBuilder HiFi DNA Assembly Master Mix (NEB # E2621L). Transfected cells were lysed in RIPA buffer (140mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... Once PCNA foci disappear the G2 phase starts and lasts until nuclear envelope breakdown (NEB). After apical mitosis ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged MyoID was generated for this study by cloning cDNA clone SD01662 from Drosophila Genomics Research Center (DGRC) into a pUAST vector using NEBuilder HiFi DNA Assembly Master Mix (NEB # E2621L). Plasmids encoding different Ds-ICD motif deletion constructs with a C-terminal V5-tag were created in this study ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Developmental Biology 2024Quote: ... of 8 or greater were subjected to Poly-A enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB catalog number E7490). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (catalog number E7760L ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were taken up in 520 µl of 1× DpnII buffer (NEB, R0543M) and incubated with 7.5 µl of 20% SDS for 1 hour shaking at 600 r.p.m ...
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bioRxiv - Developmental Biology 2024Quote: ... ∼300 ngs of genomic DNA were digested with 100U of MspI (New England Biolabs), and cleaned up using QIAquick PCR purification columns (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was treated with DNAse I (NEB, M0303S) according to manufacturer’s protocol and used for reverse transcription with iScript cDNA synthesis kit (Bio-rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Total cDNA for RT-qPCR was generated from 1.5 µg total RNA using a random primer mix and M-MuLV reverse transcriptase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of plasmid was incubated with 8 U M.SssI (NEB) at 37°C for 4 h for in vitro DNA methylation ...
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bioRxiv - Genetics 2024Quote: ... Streptavidin magnetic beads (New England Biolabs) were washed 3 times with B&W buffer I (100 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... was synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). For RNA-sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR cycles were carried out with Illumina Nextera adapter primers using the NEBNext High Fidelity 2x Master Mix (NEB, Cat# M0541S) using the following PCR program ...
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bioRxiv - Genetics 2024Quote: ... A mixture of 20 ng/μL transgene and 70 ng/μL 1 kb ladder (NEB) filler DNA was microinjected into CZ26711 young adult hermaphrodites ...
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bioRxiv - Genetics 2024Quote: ... the MseI (New England BioLabs Inc.®), were used ...
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bioRxiv - Genetics 2024Quote: ... RNA sequencing libraries were prepared with unique barcodes using the NEBNext Ultra RNA library kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The fragmented samples were purified with Expin™ PCR SV kit (GeneAll) and prepared as an NGS library with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB). The prepared samples were sequenced with MiniSeq High Output Reagent Kit (300-cycles ...
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bioRxiv - Genetics 2024Quote: ... A total of 25 µg DNA was either mock digested or digested with 30 units of RNAse H (New England Biolabs, Ipswich, MA) in 100 µl final volume at 37 °C for 6 hours ...
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bioRxiv - Genetics 2024Quote: ... the PstI (New England BioLabs Inc.®), and another frequently-cutting enzyme ...
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bioRxiv - Genetics 2024Quote: ... After DNase I treatment (New England Biolabs), RNA concentrations were determined using a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Genetics 2024Quote: ... The oligonucleotide library was procured from GenScript (USA) and subsequently amplified employing the Phusion® High-Fidelity DNA Polymerase (NEB). The amplified product and pAX198 plasmids were digested using FastDigest Bpu1102I and FastDigest BstX1 (ThermoFisher) ...
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bioRxiv - Genetics 2024Quote: The puromycin targeting sgRNA was synthesized by in vitro transcription using T7 RNA polymerase (NEB) and template oligos ...
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bioRxiv - Genetics 2024Quote: ... Reactions were then treated with 40 µg Monarch RNaseA (NEB) for 1 hour and then treated with 1.6 units of Proteinase K (NEB ...
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bioRxiv - Genetics 2024Quote: ... Next-generation sequencing libraries were prepared using NEBNext Multiplex Small RNA Library Prep Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... 1.5 µl of 25 µM dNTPs (New England BioLabs, Cat# N0446S), 1 µl molecular grade H2O ...
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bioRxiv - Genetics 2024Quote: ... DNA template was prepared by cloning into a pUC19 backbone and plasmid was linearized using SapI restriction enzyme (NEB) and purified using DNA Clean & Concentrate (Zymogen) ...
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bioRxiv - Genetics 2024Quote: ... for 1 hour and then treated with 1.6 units of Proteinase K (NEB) for a further hour before cleanup of DNA with AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Genetics 2024Quote: ... and T4 Ligase (NEB). This process created a new guide RNA expression plasmid SpCas9-2A-GFP-E171Q ...
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bioRxiv - Genetics 2024Quote: ... we subcloned the small plasmid with restriction enzymes AgeI and BssHII (New England Biolabs) into a plasmid with full length SCN5A and again Sanger sequence verified the plasmids ...
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bioRxiv - Genetics 2024Quote: ... phosphorylating the primers using T4 PNK (NEB) and digesting and ligating the insert into the pX458 plasmid using BbsI (NEB ...
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bioRxiv - Genetics 2024Quote: ... and digesting and ligating the insert into the pX458 plasmid using BbsI (NEB) and T4 Ligase (NEB) ...
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bioRxiv - Genetics 2024Quote: ... coli cells (NEB), with selection on LB agar plates using spectinomycin and ampicillin ...
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bioRxiv - Genetics 2024Quote: ... The samples were treated with Uracil-DNA-Glycosylase (UDG) from NEB to degrade the original uracilated template ...
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bioRxiv - Genetics 2024Quote: ... Gibson Assembly (NEB) was performed to ligate the resulting PCR amplicons with the pDD268 vector (Addegene #132523 ...
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bioRxiv - Genetics 2024Quote: ... After the library quantification via NEBNext Library Quant Kit (NEB), paired-end sequencing was performed on the tiles of each region with a sequencing depth of ∼2 million reads per tile using an Illumina NextSeq 500 instrument via a NextSeq 500/550 High Output Kit v2.
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bioRxiv - Genetics 2024Quote: ... KAPA HiFi Uracil+ DNA polymerase (KapaBiosystems) was used to fill in the gaps and Taq DNA ligase (NEB) was applied to seal the nicks ...
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bioRxiv - Immunology 2024Quote: ... mRNA used for comparison in NAGE gels was produced using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The PCR amplicon was purified by sequential gel extraction (1.5% agarose gel prepared in 0.5x TAE) and affinity column chromatography (Monarch® PCR & DNA Cleanup Kit, New England Biolabs). For IVT synthesis ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of 2000 U/mL DNase I (New England Biolabs) was added to bring the final reaction volume to 10 µL ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNAs were then further purified using Monarch® RNA Cleanup Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of sample from an individual cDNA reaction was mixed with 12.5 µL of 2x Q5® High-Fidelity Master Mix (New England Biolabs), 0.5 µL of each primer (0.2 µM final concentration of each primer) ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using the NEBNext Ultra II Directional RNA kit (New England BioLabs, Ipswich, MA) and sequenced with an NovaSeq 6000 at the Vanderbilt University Medical Center genomics core (VANTAGE).