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Citations for New England Biolabs :
1951 - 2000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs, USA). 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was first synthesised using the Lunascript RT Supermix (New England Biolabs, USA). qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Qualified RNA samples were subjected to sequencing library construction using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7775). Paired-end (2 × 150 bp ...
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bioRxiv - Cell Biology 2024Quote: ... Ribosomal RNA (rRNA) was then depleted using the NEBNext rRNA Depletion Kit for Bacteria (NEB, #E7850) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... HindIII (NEB, R0136L), Xba I (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... the following 30 ul PURExpress IVTT reactions (NEB) were assembled ...
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bioRxiv - Molecular Biology 2024Quote: ... and RsaI (NEB, Cat.R0167L,) and resolved by 0.8% Tris-acetate-EDTA (TAE ...
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bioRxiv - Plant Biology 2024Quote: ... mays genes were inserted into the pTYB2 vector (New England Biolabs), which was previously digested with NdeI and SmaI for fusion with an Intein/CBD sequence ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 U/μL Murine RNAse inhibitor (NEB), 400 nM 11S NanoLuc protein ...
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bioRxiv - Synthetic Biology 2024Quote: Restriction enzymes were purchased from NEB. DNA fragments were PCR amplified using a high-fidelity DNA polymerase (2x Phanta Max Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: TtgR-pET31B variants were expressed in BL21 cells (NEB) as previously described with a few minor differences30 ...
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bioRxiv - Synthetic Biology 2024Quote: ... DH10B (NEB) cells were transformed with 3μL of dialyzed reaction via electroporation ...
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bioRxiv - Biophysics 2024Quote: ... in CutSmart buffer (NEB, #B7204) with calf intestinal phosphatase (NEB ...
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bioRxiv - Biophysics 2024Quote: ... The RNA:DNA hybrid was then cleaved by RNase H (NEB catalogue # M0297L) to produce the final 22 nucleotide product ...
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bioRxiv - Cell Biology 2024Quote: ... 16.4 mL elution was taken into 40 mL ligation reaction (13 Quick ligase buffer [NEB] ...
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bioRxiv - Biophysics 2024Quote: ... pCAGEN_6×His-DNAJA2-HA was created from PCR of the ORF from pColdI_6×His-DNAJA2 and insertion into EcoRI-digested pCAGEN plasmids using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was then used to produce pCAGEN_3xFLAG-DNAJA2 in a similar manner ...
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bioRxiv - Biophysics 2024Quote: ... and lambda DNA was acquired from NEB (cat# N3011S). Oligo 1 was annealed to lambda DNA by adding a 25-fold molar excess to the DNA in an annealing buffer containing 30 mM HEPES (pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... The gene fragments were individually cloned into sensor plasmid backbones identical to those used in the previous identification of non-functional TetR(B) mutants following the manufacturers protocol for Gibson Assembly (New England Biolabs). The newly constructed plasmids carrying each of the combined mutants were then transformed into E ...
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bioRxiv - Biophysics 2024Quote: ... and Aah-OpdCpA with T4 DNA ligase (New England Biolabs), and the product was purified with phenol-chloroform extraction and ethanol precipitation and stored at −80°C until use ...
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bioRxiv - Biophysics 2024Quote: We assembled mutant libraries by combining the linearized sensor backbone with each oligo subpool at a molar ratio of 1:5 using Golden Gate Assembly Kit (New England Biolabs; 37 ◦C for 5 min and 60 ◦C for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... Libraries were prepared using NEBNext ultra II DNA library kit for Illumina (NEB Biolabs) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2024Quote: ... Reactions were dialyzed with water on silica membranes (0.025-μm pores) for 1 h before transformed into DH10B cells (New England Biolabs). Library sizes of at least 100,000 colony-forming units (cfu ...
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bioRxiv - Biophysics 2024Quote: ... Libraries were prepared using NEBNext ultra II DNA library kit for Illumina (NEB Biolabs) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was removed and stored on ice while the remaining cell pellets were digested with micrococcal nuclease (BioLabs Cat no. M0247S) to extract chromatin bound nuclear complexes ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1ug/ul BSA (New England Biolabs, B9000S), 1U/ul RiboLock (Thermo ...
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bioRxiv - Genetics 2024Quote: ... and BamHI/HindIII (NEB) at the 5’ends of the forward and reverse primers ...
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bioRxiv - Immunology 2024Quote: ... coli host cells (New England Biolabs) following an identical expression protocol ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid backbone DNA was treated with DpnI (NEB) to remove PCR template DNA ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA was purified using the Monarch RNA cleanup kit (New England Biolabs) and analyzed by agarose gel electrophoresis.
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bioRxiv - Molecular Biology 2024Quote: ... and Proteinase K (New England Biolabs, P8107S). Wells were scraped in the process of lysis buffer addition ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/mL BSA (NEB) and 40 GoldenGate digestion ligation cycles (80) ...
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bioRxiv - Biophysics 2024Quote: ... Molecular Biology Grade” (NEB) and incubated for 5 min at 3°C ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (NEB) as described above for StcE and sialidase with the exception that expression was induced with IPTG at an OD600 of 0.9 The harvested cells were then lysed using sonication ...
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bioRxiv - Microbiology 2024Quote: ... The myc epitope was added before the stop codon of blaSHV-5 by NEB Q5 Site-Directed Mutagenesis according to the manufacturer using primers JCP505/506 with pTOX5 blaSHV-5 template ...
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bioRxiv - Immunology 2024Quote: ... The HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) was used to transcribe the template DNA per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The vector was then digested with HindIII restriction enzyme (New England Biolabs), amplified ...
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bioRxiv - Genomics 2024Quote: ... An Illumina sequencing library was produced for each leaf using an optimised NEBNext Ultra II DNA library protocol (New England Biolabs, Ipswich, MA, USA). Libraries were pooled into multiplexes after independently labelling each library prior to whole genome sequencing (WGS ...
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bioRxiv - Genomics 2024Quote: ... and used for the cloning of PCR fragments with a Gibson Assembly cloning kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... This fragment was cloned into a pCGEN vector106 previously digested with KpnI (New England Biolabs), using the In-Fusion HD Cloning Kit (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... including 1800 bp upstream of the start codon and 463 bp downstream of the stop codon was amplified using Phusion DNA polymerase (NEB) and the primers included in Supplementary Table 7 ...
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bioRxiv - Genomics 2024Quote: ... 2.) Size fractionation was performed using the NEBNext Ultra II FS DNA module (New England Biolabs), 3. ...
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bioRxiv - Genomics 2024Quote: ... 3.) Library preparation was performed using the NEBNext Ultra II library preparation kit for Illumina (New England Biolabs) and 4. ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μl RppH 5000 U/μl (NEB) was added to the RNA/oligo mix ...
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bioRxiv - Molecular Biology 2024Quote: ... 1μl RNA ligase 10 000 U/μl (NEB) and 1 μl RppH 5000 U/μl (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... allowing cloning of complete vector sequences using a HiFi DNA assembly kit (NEB), following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... but with a blasticidin S-deaminase gene in lieu of SHBLE (Buck, Río Bártulos et al. 2018) introduced by NEB Hi-Fi kit as before ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was isolated using the Monarch RNA extraction procedure (New England Biolabs), including tissue grinding in liquid nitrogen and a DNAse I step ...