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Citations for New England Biolabs :
1901 - 1950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... DNA restriction enzymes were used as recommended by the suppliers (Thermo Fisher Scientific or New England Biolabs). Polymerase chain reactions (PCR ...
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bioRxiv - Cell Biology 2024Quote: ... at Bbs1 restriction site using HiFi DNA Assembly Master Mix (E2621S, NEB) 46 ...
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bioRxiv - Developmental Biology 2024Quote: ... the specific product was purified from the agarose gel using the Monarch DNA Gel Extraction Kit (NEB, T1020S). Each reverse primer contains the T3 RNA Polymerase minimal promoter sequence (shown in bold and underlined in Table 1) ...
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bioRxiv - Cell Biology 2024Quote: THP-1 monocytes were harvested and lysed for RNA extraction using the Monarch Total RNA Miniprep kit (New England Biolabs), according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...
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bioRxiv - Cell Biology 2024Quote: ... and transformed into BL21 bacterial cells (New England Biolabs). Protein expression was induced by growing a dense overnight culture ...
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bioRxiv - Cell Biology 2024Quote: ... regions surrounding the putative mutation sites were amplified using Phusion High-Fidelity polymerase (NEB) with the below primers:
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bioRxiv - Cell Biology 2024Quote: ... The fragments were assembled using NEBuilder (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Cell Biology 2024Quote: ... and 5’-ACG CGT ACT AGT CGA TCG CTT GTA CAG CTC GTC CAT G-3’ (reverse primer) and using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... (Cat#E7850L, NEB, Ipswich, MA, USA). Following elution in 8µl water ...
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bioRxiv - Cell Biology 2024Quote: ... in a reaction with DpnI (NEB) to remove PCR template material ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were amplified using -inverse PCR (Q5, NEB), using primers with overhangs containing spacer sequences ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were subsequently ligated using T4 ligase (NEB) in a reaction with DpnI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and T4 PNK (NEB: M0201S) for 1 hour in a 37C water bath ...
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bioRxiv - Cell Biology 2024Quote: T7 constructs were ordered from IDT and synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S). RNA was purified via RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... labelled with Alexa647-SNAP dye (NEB) was continuous imaged using 100 ms exposure.
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bioRxiv - Cell Biology 2024Quote: ... following the manufacturer protocols (100-6472 B1, PN 100-5875 C1) and reagents (Fluidigm,100-6297; Fluidigm, 100-5580; New England Biolabs, M0293S; TEKnova, 10221). Real-time PCR data was collected using the Biomark HD system with a 96.96 IFC chip using Fluidigm Delta-Gene Assays on the pre-amplified cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... were obtained from HeLa cells co-transfected with mGFP-Sec16L at 3 µg/µL and FLAG-TFG-SNAP at 1 µg/µL and subsequently labeled with SNAP-Cell 647-SiR (NEB, S9102S) and immunostained with anti-GM130 ...
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bioRxiv - Cell Biology 2024Quote: ... gBLOCKs and the pSNAPf plasmid (NEB, N9183S) were both digested with NheI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... were both digested with NheI (NEB, R0189S), removing SNAP and inserting the commercially obtained gene via ligation with T4 ligase (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... digested with NheI and XhoI (NEB, R0146S), and inserted into a similarly digested pSNAPf plasmid using T4 ligase ...
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bioRxiv - Cell Biology 2024Quote: The CG9590 cDNA and a C-terminal eGFP with a GHGTGSTGSGSSR linker in between were cloned into pUAS-K10attB using NEBuilder HiFi DNA Assembly (NEB). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using the ProtoScriptII First Strand cDNA Synthesis Kit (NEB; E6560S), following the manufacturer’s protocol with the Random Primer Mix and the RNA denaturation step ...
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bioRxiv - Developmental Biology 2024Quote: Reporter gene fusions for cis-regulatory analysis were made using either PCR fusion or Gibson Assembly Cloning Kit (NEB #5510S) 88 ...
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bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 13000 x g for 1 minute and the supernatant was once more purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs). We expect the “crush and soak” method to improve signal strength if low labeling densities are used during extension.
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bioRxiv - Cell Biology 2024Quote: ... The synthesized probes were then purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs) according to the manufacturer’s instructions and the quality was verified on denaturing 12-16% polyacrylamide gels.
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bioRxiv - Cell Biology 2024Quote: ... The NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used for generating the RNA-Seq library for each sample ...
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bioRxiv - Cell Biology 2024Quote: ... pNGS2 was digested with EcoRV-HF restriction enzyme (New England Biolabs, USA) and purified by agarose gel electrophoresis followed by extraction using FastGene Gel/PCR Extraction kit (NIPPON Genetics ...
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bioRxiv - Cell Biology 2024Quote: ... a five-fold molar excess of oligonucleotides bearing either 8-oxo-G or G was mixed with the phage-extracted circular single-stranded DNA in 1=×=Phusion HF Buffer (New England BioLabs). After denaturation at 90=°C for 2=min followed by rapid cooling ...
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bioRxiv - Cell Biology 2024Quote: ... Library preparation was slightly different and performed using the NEBNext® rRNA Depletion kit and protocol (NEB; P/N: E7850X) per manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... using Phusion High-Fidelity DNA polymerase and Taq DNA ligase (New England BioLabs). The reaction took place at 50=°C for 30=min ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... and A549 Chk2-/- cells using the Monarch® Genomic DNA Purification Kit (NEB). Prior to Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized via reverse transcription from ~500 ng of RNA using LunaScript RT Supermix (New England Biolabs). Transcripts of interest were subsequently quantified using Luna qPCR master mix (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... was linearised using by BbsI-HF (New England Biolabs, #R3539S), then the guide oligonucleotides were annealed and inserted into the vector using Ligation high Ver.2 (TOYOBO ...
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bioRxiv - Cell Biology 2024Quote: ... and purified with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490). The quality of the RNA samples was examined using the Agilent 2100 Bioanalyzer system (Agilent Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification was performed using Q5 high-fidelity polymerase (NEB) to amplify the targeted regions ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760) was then used to prepare libraries for paired-end (2x150bp ...
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bioRxiv - Cell Biology 2024Quote: ... The six pieces were assembled with the Golden Gate BsaI-HF v2 assembly kit (NEB). Three guide RNA (gRNA ...
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bioRxiv - Cell Biology 2024Quote: ... Transcripts of interest were subsequently quantified using Luna qPCR master mix (New England Biolabs) and a Quant Studio 6 Flex instrument ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting PCR product was treated with DpnI (NEB), gel purified using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... colonies were screened using Q5 High Fidelity DNA Polymerase (NEB, M0491L) and at least one primer upstream or downstream of the DNA region used for recombination ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg of plasmids were then digested overnight with the restriction enzyme CspCI (NEB, #R0645S) in a total volume of 50 μL ...
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bioRxiv - Cell Biology 2024Quote: ... and was assembled with the PCR fragments in NEBuilder® HiFi DNA assembly reaction (NEB) and transformed into DH5α E ...
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bioRxiv - Cell Biology 2024Quote: ... and was assembled with the PCR fragments in NEBuilder® HiFi DNA assembly reaction (NEB) and transformed into DH5α E ...
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bioRxiv - Cell Biology 2024Quote: ... Q5 DNA polymerase (NEB) with primer pairs covering the entire predicted coding region (primer combinations listed in Table S1).
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bioRxiv - Cell Biology 2024Quote: ... and was assembled with the PCR fragments in NEBuilder® HiFi DNA assembly reaction (NEB) and transformed into DH5α E ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine was then removed by washing cells with culture media and blocking of existing CENP-A was performed by treatment with 10 mM SNAP-Cell® Block BTP (S9106S, NEB) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Gibson assembly was performed at 50 °C for 30 min using the HiFi DNA Assembly Master Mix (#E2621L, New England Biolabs). Resulting plasmid pAC-AfaI-Cm was transformed into S ...