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Citations for New England Biolabs :
1851 - 1900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 2 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... and then immediately followed by Library prep using NEBNext Ultra II kit (NEB, E7770). Libraries were pooled and sequenced using a H75 kit from Illumina in a NextSeq500 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were quantified using the NEBNext Library Quant Kit (NEB, E7630S), then pooled at same molarity and paired-end sequenced with a P2-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Each 50 µL PCR2 reaction contains 25 µL of 2xQ5 Master Mix (New England Biolabs, M0494L), 5 µL of each index primer (N7xx or S5xx) ...
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bioRxiv - Developmental Biology 2024Quote: The PCR-based Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) was used to perform truncation (constructs L-P ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s specifications (New England Biolabs, Beijing LTD, Beijing, China). Total proteins were subjected to SDS-PAGE and then electrophoretically transferred onto a nitrocellulose membrane for western blotting.
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bioRxiv - Developmental Biology 2024Quote: ... Q5 High-Fidelity polymerase (NEB) was used to linearize the backbone vector (LaZ_IF_Fw and LacZ_IF_Rev primers ...
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bioRxiv - Developmental Biology 2024Quote: ... The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB; M0318S). The pXPR_050 plasmids were then transformed into Stbl4 competent cells (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... pXPR_050 plasmids were digested using the Bsmb1 restriction enzyme (NEB; R0580). The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... or pMAL-c2x (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... or Amylose Resin High Flow (New England Biolabs) respectively according to the manufacturers’ instructions.
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bioRxiv - Cell Biology 2024Quote: ... Each 100 µL PCR1 reaction contains 50 µL of 2xQ5 Master Mix (New England Biolabs, M0494L), 0.2 µL of MgCl2 (stock concentration at 1M) ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were performed using the Q5 DNA polymerase (NEB) in a GeneAmp PCR system 2400 (Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... The plasmids were constructed using T4 DNA ligase (New England Biolabs) with a ratio of 3:1 of insert to backbone ...
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bioRxiv - Microbiology 2024Quote: ... coli NiCo21(DE3) (NEB BioLabs) was used to express recombinant proteins ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of 10 mM MnCl2 and 1 μl (400 units) of λPP (New England Biolabs, Ipswich, MA). Untreated lysates received 1 μl of H2O in place of λPP ...
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bioRxiv - Microbiology 2024Quote: ... linear plasmid backbone was generated by restriction digestion with SmaI (NEB). Plasmid backbone DNA was treated with DpnI (NEB ...
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bioRxiv - Microbiology 2024Quote: ... mApple was cloned in place of the eYFP gene using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and BstXI and NotI restriction sites as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and ligated using Instant Ligase Sticky-end Ligase Mix (New England Biolabs). Ligations were transformed into NEB10β competent cells following the manufacturer recommendations (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids and PCRs were digested with the appropriate restriction enzymes (New England Biolabs), purified ...
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bioRxiv - Microbiology 2024Quote: ... The purified SOE PCR product was double-restriction enzyme-digested and ligated into a pk18mobsacB vector previously double-digested with the corresponding enzymes and dephosphorylated with rSAP (NEB, UK). The constructs were transformed into E ...
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bioRxiv - Microbiology 2024Quote: ... The molecular weight standards used were unstained broad range protein standards (NEB) and then the gel was run at 100 volts until the dye front was 1 cm from the bottom of the gel and then stained one hour in Coomassie blue stain (50% methanol ...
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bioRxiv - Microbiology 2024Quote: ... the DNA in 11.5 µL in a PCR tube was end-repaired by addition of 1.75 µL NEB Ultra II End Repair Buffer (NEB) and 0.75 µL Enzyme Mix followed by thorough mixing via pipetting ...
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bioRxiv - Microbiology 2024Quote: ... coli C2925 (NEB). Cloned gene expression was induced by inclusion of the indicated concentration of L-tryptophan (Trp ...
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bioRxiv - Microbiology 2024Quote: ... acidocaldarius DSM 639 wild type using the primers (Eurofins Genomics) listed in supplementary Table 4 employing the Q5 polymerase (NEB, USA) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... PCR products and the respective antibiotic resistance gene were used for Gibson assembly (NEB, USA) (38) ...
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bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of D072 primer (Table 1) and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 μCi) ...
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bioRxiv - Microbiology 2024Quote: ... On-bead PCR indexing-amplification was performed using custom-ordered indexing primers (IDT) matching the Illumina Nextera Index Kit sequences and 2x Phusion Master Mix (NEB). PCR reactions were amplified for 9-11 cycles ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Microbiology 2024Quote: ... Inserts were amplified by PCR from diluted ΦKZ lysates and joined into the linearized vector by Hi-Fi DNA Assembly (NEB). The resulting vectors were used to transform E ...
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bioRxiv - Microbiology 2024Quote: ... Genes of interest were PCR amplified to include either their native or heterologous promoter and cloned using Gibson Assembly kit (NEB) into integration vectors pDG1662 (for insertion into the amyE locus) ...
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bioRxiv - Microbiology 2024Quote: ... beads were incubated with 10 μM SNAP-Surface Alexa Fluor 488 (NEB) for 1 hour at 4°C prior to elution ...
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bioRxiv - Microbiology 2024Quote: ... the protein eluted from MonoQ 5/50 GL was incubated with 10 μM SNAP-Surface Alexa Fluor 647 (NEB) for 1 hour at 4°C prior to gel filtration.
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM DTT) and a wash with 10 μL streptavidin (NEB). The chamber was further washed with 10 μL TIRF buffer followed by a 1-minute incubation with 2 μL of microtubules diluted in 8 μL TIRF buffer supplemented with 50 mM KCl and 1.25 mg/mL casein (TIRF-Casein) ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Microbiology 2024Quote: Test PCRs on cDNA were performed in a 50 µL scale using 1.0 µL precipitated cDNA in the presence of 1 x High-Fidelity PCR MasterMix (NEB), 2 µM forward primer with native barcode and 2 µM reverse primer with native barcode ...
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bioRxiv - Microbiology 2024Quote: ... followed by treatment with polynucleotide kinase (NEB). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... coli B strain was dephosphorylated with 0.4 U/µL QuickCIP (NEB) in 1 x rCutSmart buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... RNA was then purified again and the eluate was subjected to circularization with T4 RNA Ligase 1 (NEB). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Immunology 2024Quote: ... and PCR amplification of the gRNA insert was done on 2000 genomes per sample using Q5® Hot Start High-Fidelity DNA Polymerase (NEB # M0493) with P5 and P7 primers that included the Genewiz partial adapter sequence (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... 100 fmol of library per 50 µL PCR reaction was amplified using Q5 HotStart DNA Polymerase (New England Biolabs M0493S) in 8 reactions with a 50 °C – 62 °C gradient annealing step using the following program:
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bioRxiv - Cell Biology 2024Quote: ... The RNase III-treated samples were pre-incubated with 4 U of RNase III (New England Biolabs, Ipswich, Massachusetts, USA, M0245S) in reaction supplemented by 20 mM MnCl2 at 15 °C for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... were assembled in 3 separate reactions for 15 minutes at 50 °C or 55 °C using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs E2621S) alongside a control reaction omitting the insert ...
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bioRxiv - Cell Biology 2024Quote: ... The same amount of gDNA was digested with RSAI and HINFI enzymes (NEB) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and treated for 10 min at RT by RNase III enzyme (2 U of RNase III (Short Cut, New England BioLabs, Massachusetts, USA, M0245S) with 20 mM MnCl2 in 1× reaction ShortCut buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... Background assembly was measured by transformation of NEB 5-alpha competent cells (New England Biolabs C2987I) before cleanup of the assembly reactions using AmpureXP magnetic beads (Beckman Coulter A63880) ...
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bioRxiv - Cell Biology 2024Quote: ... using HiFi DNA Assembly Master Mix (E2621S, NEB) 46 ...