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Citations for New England Biolabs :
1801 - 1850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the kanamycin resistance cassette was replaced with a chloramphenicol resistance cassette using ITA and the region downstream of the promoter was deleted using site-directed mutagenesis using KLD enzyme mix (NEB). The pEMH120 empty vector was constructed from a pTGR5 (88 ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α(λpir) cloning strain (NEB) was propagated at 37°C in LB (1% tryptone ...
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bioRxiv - Microbiology 2024Quote: ... Tags (6x His or HA) were introduced using site-directed mutagenesis with KLD enzyme mix (NEB).
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bioRxiv - Microbiology 2024Quote: ... coli DH5α(λpir) (NEB) competent cells by heat shock (42°C for 40 seconds) ...
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bioRxiv - Microbiology 2024Quote: Colony PCR was performed using either Q5 (NEB) or Sapphire master mix (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Q5® High-Fidelity DNA Polymerase (New England Biolabs) was used according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... hepatitis delta virus (HDV) ribozyme and simian virus 40 (SV40) poly-A sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) at 50 °C for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... The resulting GlnA1_opt PCR-product and vector pRS375 were restricted with NdeI and BamHI (NEB, Schwalbach, Germany); the resulting pRS375 vector fragment and the GlnA1 fragment were ligated resulting in pRS1841 ...
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bioRxiv - Microbiology 2024Quote: ... which was linearized with BbsI (R3539, NEB, USA). The p3xFlag-GGCX (Flag-GGCX ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, #E7530L). All libraries were prepared using manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Q5 Reaction Buffer Pack (NEB, B9027S). For PCR #1 ...
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bioRxiv - Cell Biology 2024Quote: ... one of the immunoprecipitated samples was treated with 400 units of Lambda protein phosphatase (New England Biolabs, P0753S) at 30 °C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... All PCR was performed using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493L) and the Q5 Reaction Buffer Pack (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... 15µl to 25µl of extracted proteins in NuPAGE buffer and 0.5µl protein ladder (BioLabs) were loaded into a Bio-Rad 4-15% Mini-PROTEAN precast polyacrylamide gel ...
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bioRxiv - Cancer Biology 2024Quote: ... and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253, New England Biolabs) was added and the reaction was incubated at 42 °C for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted and cloned into BsmBI-digested (NEB)/ CIP-treated (NEB) plentiCRISPR v2 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted and cloned into BsmBI-digested (NEB)/ CIP-treated (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and underwent paired end sequencing in a NextSeq500 or HiSeq PE150 (Novogene).
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bioRxiv - Cancer Biology 2024Quote: ... coli (NEB) as described above for StcE and sialidase ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB). After library QC analysis using Bioanalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA sequencing library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using LUNA Universal qPCR Master Mix (New England Biolabs) on the 7500 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) according to manufacturer’s instructions in combination with a poly(A ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphorylated with T4 polynucleotide kinase (NEB). First ...
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bioRxiv - Cancer Biology 2024Quote: ... For the minigene constructs the intron and parts/complete adjacent upstream and downstream exons were PCR-amplified from K562 genomic DNA using Phusion Hot Start Flex polymerase (New England Biolabs) and cloned by the Hot Fusion (61 ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Cancer Biology 2024Quote: ... Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs) and are listed in Supplementary Table S4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mutations and insertions were introduced by site directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs). Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified HMGCR was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI (New England Biolabs, R0136) and XhoI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and library quantification using NEBNext Library Quant kit (NEB), the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... PolyA RNA was extracted using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and used as input for RNA-seq ...
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bioRxiv - Cancer Biology 2024Quote: ... or 5,000 U/mL PNGase F (P0704S, New England BioLabs) in growth media for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... After dephosphorylation with T4 Polynucleotide Kinase (NEB, #M0201S) the adapter Linker-1 (5’ rAppCTGTAGGCACCATCAAT/3ddC/ 3’ ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using dual-end indexing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645L) using the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA (>300 ng) was reverse transcribed using the Lunascript RT Supermix Kit (NEB, E3010).
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bioRxiv - Cancer Biology 2024Quote: ... using the Luna Universal qPCR Master Mix (NEB, M3003), and included initial enzyme activation for 3 minutes at 95 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and barcoded libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were pooled and single-end sequenced (1×75 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A) RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and barcoded libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... pA-Tn5 transposome was incubated with 150ng HMW Lambda DNA (NEB) in digestion buffer (5XTAPS-MgCl2-PEG8000 ...
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bioRxiv - Cancer Biology 2024Quote: ... prior to incubation with 1% SDS and proteinase K (20mg/mL, NEB) to inactivate and degrade pA-Tn5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB; E7765), and a minimum of 20 million raw reads were obtained ...
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bioRxiv - Developmental Biology 2024Quote: ... both CRISPRi plasmid pools and genomic DNAs were PCR amplified with Miseq_Adaptor primers (Table S5) using Q5 High-Fidelity DNA Polymerase (NEB; M0491L). PCR samples were run on a 2% agarose gel and purified using a Gel Extraction Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Eluted DNA was amplified using NEBNext Ultra II PCR Master Mix (NEB) and purified using AMPure XP beads ...
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bioRxiv - Developmental Biology 2024Quote: ... The repair template was made by NEB assembly of upstream flanking sequence and degron from the repair template of degron::gfp and a synthetic gene fragment from IDT that contains a 3xFLAG tag and the downstream flanking sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 7 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified by Nanodrop and 1 µg of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (NEB, E6350) and then immediately followed by Library prep using NEBNext Ultra II kit (NEB ...