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Citations for New England Biolabs :
1751 - 1800 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... aegypti embryos were injected with a microinjection mix containing 402.5 ng/µL SpCas9 protein (New England Biolabs), 40 ng/µL of each of three sgRNAs (x1_30rev ...
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bioRxiv - Cell Biology 2024Quote: ... existing CENP-A-SNAP proteins were labelled for 30 min using 10 µM SNAP-Cell Block (NEB) and cells were exposed to media containing 5 µM S-trityl-L-cysteine (STLC - Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and used in a 1:1:1 molar ratio Golden Gate assembly reaction with BsaI (NEB) and T4 ligase (NEB ...
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bioRxiv - Genomics 2020Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 minutes using NEB Quick T4 DNA Ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckmann Coulter ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was then treated with DNase for 10 min at 37 °C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Synthetic Biology 2019Quote: Extension reactions on single-stranded DNA were performed similarly to the reactions with Duplase enzymes described above for 60 minutes at 60°C using 2U Vent (NEB, Ipswich, MA), 2U Deep Vent (NEB) ...
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bioRxiv - Systems Biology 2019Quote: ... The cDNA inserts were PCR-amplified using primers specific for either the N- or C-terminal libraries using Phusion DNA polymerase (New England Biolabs, Ipswich, MA). Amplicons were PCR-purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... the poly (A) product was broken into pieces at 94 °C for 5-7 min using the Magnesium RNA Fragmentation Module (NEB, MA, USA). The RNA fragments were then reverse-transcribed using the SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... These were ligated to modified Illumina P1 and P2 adapters overnight at 16 °C with 1,000 units of T4 ligase (New England Biolabs, Beverly, MA, USA), followed by a 10-minute heat-deactivation step at 65 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated nucleotides from the non-ligated DNA ends were removed by incubating the Hi-C libraries (2 μg) in the presence of 6 U of T4 DNA polymerase (NEB; Cat#: M0203L) in NEBuffer 2.1 supplied with 0.025 mM dATP (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... The Hi-C library for Illumina sequencing was prepped by NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The Hi-C library for Illumina sequencing was prepared using the NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Each plug was melted in 200 µl TE at 68 °C for 30 minutes and then digested using β-agarase I (BioLabs, M0392L) at 42 °C overnight ...
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bioRxiv - Physiology 2022Quote: ... from epithelium or sensory neurons underwent digestion for 10 hours at 37°C with MspI restriction enzyme (New England Biolabs, Ipswich, MA), followed by purification with AMPure XP (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... the tdTomato gene (codons optimized for expression in C. burnetii) was amplified by PCR with Q5 polymerase (New England Biolabs, Frankfurt, Germany) using primers a533 (5’-GATTTAAGAAGGAGATCTGCAGATGGTGTCAAAAGGAG-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... The solution was transferred to 42°C for 15 min and incubated overnight in 3 U of β-agarase I (New England Biolabs, M0392). Next ...
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bioRxiv - Physiology 2021Quote: ... and 72 °C for 5 min) using a Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs, Ipswich, MA, USA) and primers containing the restriction sites of SpeI or BglII for subsequent subcloning (F ...
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bioRxiv - Developmental Biology 2022Quote: ... <200nt isolates were immediately frozen at -80⍰°C while >200nt isolates were used as template for cDNA synthesis (LunaScript RT Mastermix Kit, New England Biolabs, MA, USA) and subsequent doublesex sexing PCR as previously described (25) ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 μM DTT) and incubated for 30 minutes at 37°C in the presence of 1000 gel units of MNase (#M0247S, NEB, Ipswich, MA) in 300 μl volume of buffer B ...
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bioRxiv - Immunology 2020Quote: ... G and C and lower case ‘g’ represents RNA bases) and the uracil-containing primers subsequently removed by treatment with UDG (NEB, Hitchin, UK). TRA and TRB sequences were amplified using a pair of 5’ ‘step-out’ primers specific for the SMART oligo (long 5’ primer - CTA ATA CGA CTC ACT ATA GGG CAA GCA GTG GTA TCA ACG CAG AGT ...
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bioRxiv - Synthetic Biology 2021Quote: All plasmid vectors were prepared by Qiagen midi prep and 10-20 ug of DNA linearized by digest with KpnI-HF and XbaI restriction enzymes at 37°C in NEB Cutsmart buffer with enough units of activity to generate ~5X overdigest in two hours (New England Biolabs, Ipswich, MA). Reaction was stopped and DNA partially purified by NaCl/Isopropanol precipitation followed by three 70% Ethanol washes and resuspension in 1 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A-tails were added by mixing the purified end-repaired DNA with dATPs and Klenow exonuclease and incubating at 37° C for 30 minutes (NEB, Ipswich, MA) and then purified using the Qiagen QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The appropriate N-or C-terminal poly-histidine or strep-tags were engineered onto BchB using Q5 site-directed mutagenesis (New England Biolabs, Ipswich, MA). The primers used to generate the corresponding sequence change are listed (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... grown to an ideal OD and induced with IPTG as per manufacturer’s instructions at 25°C (Sigma Aldrich for pGEX and New England Biolabs for pMal). Cells were resuspended in lysis buffer [2.5 mM MgCl2 ...
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bioRxiv - Genetics 2021Quote: PCR products (150 – 200 ng) were digested for 30 min at 37°C in 20 ul 1X restriction enzyme buffer (NEB buffer v3.1) containing 500 ng Cas9 (Alt-R® S.p ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then 3’dephosphorylated by denaturing at 65°C for 5 min and incubating with T4 PNK (NEB, catalog no. M0201S) in a 10 μL reaction (7 μL precipitated RNA ...
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bioRxiv - Microbiology 2021Quote: Vector pFLD was digested with PmlI at 37°C for 2 h and dephosphorylated with 5 U Antarctic phosphatase at 37 °C for 30 min as recommended by the manufacturer (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... The Hi-C library for Illumina sequencing was prepped using the NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Adapter ligation (AMX) was performed at RT (20 °C) for 20 min using NEB Quick T4 DNA ligase (New England Biolabs, MA, USA). The reaction mixture was purified using 0.6X AmPure beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapter ligation was performed at 20°C for 10 min, with Adapter Mix (AMX, ONT) and Blunt/TA Ligase Master Mix (New England BioLabs, cat# M0367). After a final 1X clean-up and washing of the beads with Adapter Binding Buffer (ABB ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Hi-C library for Illumina sequencing was prepped by NEBNext Ultra II DNA library Prep Kit for Illumina (NEB Cat# E7645S) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 16 °C for 4 h in the presence of 1.15x of T4 ligation buffer (New England Biolabs, catalog no. B0202S) and 100U T4 ligase ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA was then treated with DNase for 10 min at 37◦C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Genomics 2024Quote: ... Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). Small RNA PCR amplicons were size selected on 10% polyacrylamide non-denaturing gels ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, ref E5520S). Reaction products were amplified in provided bacteria and purified using QIAprep Spin Miniprep Kit.
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Lentiviral related PLD3/4 plasmids were cloned into pBOBI vector that has a C-terminal FLAG tag after digestion by restriction enzymes BamHI and XhoI (NEB R3136S, R0146S). All these plasmids were prepared with endotoxin-free kit (Takara 740426 ...
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bioRxiv - Microbiology 2023Quote: ... and amplified with the primers (5’-CAA GAC TAG TGG AAG CGG AGC TAC TAA CTT CAG CCT GCT GAA GCA GGC TGG CGA CGT GGA GGA and 5’-NNN NAC GCG TCT AGC CTT CCC AGA CGT ACC C) using high-fidelity Phusion polymerase (NEB, Cat# M0530S). The PCR fragment was digested with BmtI and MluI ...
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bioRxiv - Microbiology 2023Quote: ... the Strep-tag® II sequence was inserted at the C-terminus via Phusion® High-Fidelity DNA Polymerase mutagenesis PCR (New England Biolabs) using the primers pYS14_GA_F/ 5’ phosphorylated flaakstrp14R to amplify the whole plasmid ...
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bioRxiv - Microbiology 2023Quote: ... A total of 3 μg of RNA was denatured at 65°C for 10 min with equal volume of RNA loading dye (New England Biolabs, MA, USA). RNA samples ...
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bioRxiv - Genetics 2023Quote: ... Five microgram samples were individually digested overnight at 37°C with different methylation-sensitive restriction enzymes (HaeIII, AvaII, HpaII, Sau3AI and PspGI) (New England Biolabs; Ipswitch, MA). A control digest was also prepared using McrBC for 2 hours at 37°C (Xie et al. ...
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bioRxiv - Microbiology 2023Quote: ... DNA was ligated for 4 h in a water bath at 16 °C (30 Weiss Units of T4 DNA Ligase, M0202 New England BioLabs® Inc). 300 μg of Proteinase K was used to reverse the cross-linking overnight at 65°C ...
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bioRxiv - Genetics 2023Quote: ... 5’ and 3’ homology arm flanking the C-terminal insertion site was amplified from N2 genomic DNA using the Q5® High-Fidelity DNA Polymerase (NEB, M0491L), followed by HiFi DNA assembly (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... attB plasmid containing genes for tdMCP-protein fusions and a MS2-circRNA barcode were digested overnight at 37°C to remove existing barcode sequence (2 μg plasmid, 5 μL 10x CutSmart buffer, 2 μL BsrGI-HF (NEB cat#R3575S), nuclease-free water to 50uL) ...
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bioRxiv - Molecular Biology 2024Quote: ... was digested by combining the following and incubating overnight at 37°C: 2 μg plasmid + 5 μL CutSmart buffer (10x) + 2 μL AflII (NEB cat# R0520S) + 2 μL BlpI (NEB cat# R0585S ...
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bioRxiv - Bioengineering 2024Quote: ... Reactions were incubated at 16°C for 16 hours and purified with Monarch® RNA Cleanup Kits (New England Biolabs, Cat# T2030) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into overexpression vectors under act5 promoter with either N-terminal or C-terminal FLAG tags using Gibson Assembly® Master Mix (NEB, E2611L) at 50 °C for 1 hr ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Samples of ∼200 ng genomic DNA were digested with 20 units of MspI (C↓CGG) and 10 units of AluI (AG↓CT) (New England Biolabs, United Kingdom) at 37°C overnight (∼16 hours) ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl 40 U μl-1 RNase Inhibitor (NEB, M0314), and 1 μl T4 RNA ligase 2 (truncated K227Q ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 ul T4 RNA ligase 1 (10U/ul – NEB M0204), 0.5 ul 100 uM 5’ RNA linker (Blocked at 5’ end & contains NNNN at 3’ end for barcoding) ...