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Citations for New England Biolabs :
1751 - 1800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... coli (New England Biolabs C2527H) underwent transformation with wild-type α-syn/pET21a ...
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bioRxiv - Cell Biology 2024Quote: Proteins were expressed in Escherichia coli T7 Express lysY/Iqcells (New England Biolabs) in 2×TY medium at 37°C to an OD600 of 0.8–1.2 before inducing protein expression by addition of 0.4 mM isopropyl β-D-thiogalactopyranoside (IPTG) ...
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bioRxiv - Cell Biology 2024Quote: 500 ng of total RNA per sample were utilized as input for mRNA enrichment procedure with NEBNext® Poly(A) mRNA Magnetic Isolation Module (#E7490L, New England Biolabs, US) followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L ...
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bioRxiv - Cell Biology 2024Quote: ... All steps were performed as recommended in user manual E7760 (Version 1.0_02-2017; NEB), except that all reactions were downscaled to 2/3 of initial volumes.
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bioRxiv - Cell Biology 2024Quote: ... using NEBNext Multiplex Oligos for Illumina – 96 Unique Dual Index Primer Pairs (#6440S, New England Biolabs, US). All generated cDNA libraries were amplified with 7 cycles of final PCR.
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA and cDNA PCRs were carried out using OneTaq polymerase (New England Biolabs, Ipswich, MA, USA) as per instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The WT PACT expression vector was used as template for constructing p38 mutant PACT clone by using Q5 site-directed mutagenesis kit (New England Biolabs, E0554S). Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were then inverted onto a 35μL drop on parafilm of NEB Blunting Reaction (NEB, E1201): (1mM dNTPs ...
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bioRxiv - Cell Biology 2024Quote: The plasmids used in this study were obtained from the sources as noted in Table 2 or made by either restriction digest and ligation or PCR and NEBuilder HiFi DNA assembly (New England Biolabs E5520S). Insert sequences were ordered as custom GeneBlocks from IDT or isolated from existing plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng cDNA was amplified by PCR for 30 cycles using Phusion High Fidelity DNA Polymerase (M0530L; New England Biolabs) using the following primers:
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bioRxiv - Cell Biology 2024Quote: ... with primers BA3187/BA3188 and cloned into RSFDuet-1 using BamHI/EcoRI sites with the NEBuilder HiFi DNA Assembly kit (NEB) (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs; Ipswich, MA) for 10–20 min in a 37°C water bath ...
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bioRxiv - Cell Biology 2024Quote: ... Half of the DNA was methylated in vitro with the CpG methyltransferase M.SssI (M0226L, New England Biolabs, USA) using S-adenosylmethionine (SAM ...
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bioRxiv - Cell Biology 2024Quote: ... approximately 1 μg of gDNA each sample was subject to bisulfite conversion for shotgun library construction (NEB Ultra II) and Illumina HiSeqX PE150 sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and ProtoScript® II reverse transcriptase (M0368L, New England Biolabs). RT-qPCR was performed on a LightCycler 480 instrument (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... the RNA underwent quantification and quality assessment before library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs, US). All steps were performed as recommended in user manual E7760 (Version 1.0_02-2017 ...
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bioRxiv - Cell Biology 2024Quote: ... The purified cDNA was added to a mixture of 10× terminal transferase buffer (NEB, no. M0315S), 2.5 mM CoCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 U/μL Taq DNA polymerase (NEB) for incubation at 25°C for 60 min and heating at 68°C for 30 s and then at 75°C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... RNF43 mutants were generated by PCR-subcloning using Q5 High-Fidelity 2× Master Mix (NEB). Domain swapped constructs were generated by in-fusion cloning (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 U/μL terminal transferase (NEB, no. M0315S). After incubation at 37°C for 30 min and termination at 70°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA resistant mCherry-ALG-2 was created by mutagenesis (primers sequences 5’-ATTTCGATGTTTGACCGTGAGAAC-3’, 5’-AATGGACCTGACAGTCACTGGATTAAA-3’) using a Q5 Site-Directed Mutagenesis Kit (E0554S, NEB). The plasmid encoding IST1-GFP is as described (63 ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmid encoding TMEM192-GCAMP7 was constructed by cloning TMEM192 and GCAMP7 sequence into pcDNA 3.1/Zeo (+) using Gibson Assembly (E2611, NEB). The plasmids encoding GCAMP7-TRPML1 ...
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bioRxiv - Cell Biology 2024Quote: ... Gibson assembly was performed at 50 °C for 30 min using the HiFi DNA Assembly Master Mix (#E2621L, New England Biolabs). Resulting plasmid pAC-AfaI-Cm was transformed into S ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs, USA). 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified from mouse cDNA by polymerase chain reaction (NEB, M0492L) using primers below:
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was first synthesised using the Lunascript RT Supermix (New England Biolabs, USA). qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Qualified RNA samples were subjected to sequencing library construction using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7775). Paired-end (2 × 150 bp ...
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bioRxiv - Cell Biology 2024Quote: ... Ribosomal RNA (rRNA) was then depleted using the NEBNext rRNA Depletion Kit for Bacteria (NEB, #E7850) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids in which multiple cdk5 phosphorylation sites in the DCX C-terminus were replaced with non-phosphorylatable or phosphomimetic residues were cloned by Gibson assembly (New England Biolabs) of a synthetic DNA fragment carrying these changes (synthesized by Twist Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... newly deposited CENP-A was labelled with 3 µM SNAP-Cell® 647-siR (S910102S, NEB) for 30 mins before washing excess with media and allowing to grow for a further 30 mins ...
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bioRxiv - Cell Biology 2024Quote: ... and was assembled with the PCR fragments in NEBuilder® HiFi DNA assembly reaction (NEB) and transformed into DH5α E ...
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bioRxiv - Immunology 2024Quote: ... the plasmids were linearized by digestion with SpeI (New England BioLabs), followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Immunology 2024Quote: ... Resulting mRNA products were purified using Monarch RNA Cleanup Kit (New England BioLabs), visualized on 1% TBE gel ...
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bioRxiv - Immunology 2024Quote: ... pUC57-mini plasmids harboring the WT or mutated coding sequence downstream of T7 promoter were first transcribed into mRNA using the HiScribe T7 ARCA mRNA Kit (New England BioLabs) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: N-linked glycans were enzymatically released from purified mouse THP using PNGase-F kit (catalog no P0709S, New England Biolabs). N-glycans were then purified from the reaction mixture containing denaturing buffer and de-N-glycosylated proteins by solid phase extraction method using Sep-Pak C18 (1 cc Vac-cartridges ...
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bioRxiv - Immunology 2024Quote: ... were prepared and sequenced at Azenta (South Plainfield, NJ) using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The sequencing libraries were validated on the Agilent TapeStation ...
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bioRxiv - Microbiology 2024Quote: ... and ASP14_lasA_KO_DN_F and ASP15_lasA_KO_SacI_DN_R (downstream) (Table S3) and cloned into pEXG2-Tc with Gibson assembly (New England Biolabs). pEXG2-Tc-ΔlasA (Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Microbiology 2024Quote: ... coli NiCo21(DE3) (NEB BioLabs) was used to express recombinant proteins ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplifications were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs) with supplementation with High GC-enhancer to the reaction following the manufacturer’s instructions and primers synthesized by Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified amplicons was quantified by qPCR using the NEBNext Illumina Quant kit (NEB). The forward and reverse reads were demultiplexed and fused barcodes were quantified through alignment against custom barcode and primer sequences using Bowtie2 (V2.3.4.1 ...
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bioRxiv - Microbiology 2024Quote: ... The CUP1 promoter sequence was amplified from the iBFG-Y2H vectors (primers oHM487/488) and cloned into N2H N1 and N2 vectors digested with SpeI/SacI using NEBuilder assembly (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were then size selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
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bioRxiv - Microbiology 2024Quote: ... using Phusion polymerase (NEB). All strains and plasmids were verified using sequencing ...
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bioRxiv - Microbiology 2024Quote: ... After DNA isolation libraries were prepared with the NEBNext ULTRA II DNA Library Prep Kit for Illumina (NEB, US) according to manufacturer’s specification with the NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s specification with the NEBNext Multiplex Oligos for Illumina (NEB, US). The resulting libraries were sequenced on the Illumina NovaSeq 6000 platform in SP mode with 2x100 bp output at the Utrecht Sequencing Facility (USEQ ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x Apyrase buffer (NEB) and 1 µl of Apyrase (M0398S ...