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Citations for New England Biolabs :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA (300 ng) was depleted of ribosomal RNA using the NEBNext rRNA Depletion Kit (NEB Cat#E6310). rRNA-depleted RNA was purified using RNAClean XP beads (Beckman-Coulter Cat#66514) ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Neuroscience 2024Quote: ... 25-50 ul Protein G Magnetic Beads (NEB #S1430S) were incubated with 3ug GFP (D5.1 ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... C terminal and αPKC binding region) or PICK1 (BAR domain) were made with NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Mutations of PICK1 (KD-AA and 5K-E ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA template and LUNA master mix (NEB) were added into white PCR reaction tubes and placed in a CFX Maestro (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2024Quote: ... the K129A mutation was introduced using the Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... and the codon-optimized APEX2-V5 sequence (see above) were amplified using the Q5 hot-start high-fidelity DNA polymerase (New England Biolabs) and inserted into the pJFRC81-10xUAS-IVS-Syn21-GFP-p10126 vector (Addgene #36432 ...
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bioRxiv - Neuroscience 2024Quote: ... to replace the GFP sequence using NEBuilder HiFi DNA assembly master mix (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... a ∼1500bp genomic sequence flanking the Ten-m start codon (∼750bp each side) was amplified using the Q5 hot-start high-fidelity DNA polymerase (New England Biolabs) and inserted into the pCR-Blunt-TOPO vector (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... The codon-optimized APEX2-V5 sequence was synthesized as a gBlock (Integrated DNA Technologies) and inserted into the TOPO genomic sequence plasmid using the NEBuilder HiFi DNA assembly master mix (New England Biolabs). CRISPR guide RNA (gRNA ...
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bioRxiv - Neuroscience 2024Quote: ... and amplified the Gek or Syd1 coding sequences using the Q5 hot-start high-fidelity DNA polymerase (New England Biolabs). The verified coding sequences were then assembled into a modified pUAST-attB vector ...
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bioRxiv - Neuroscience 2024Quote: ... a V5 tag was inserted after the start codon of Ten-m cDNA (isoform B) in the plasmid pUAST-attB-Ten-m5 using the Q5 site-directed mutagenesis kit (New England Biolabs). To generate the UAS-V5-Ten-m-ΔICD and UAS-V5-Ten-m-ΔECD constructs ...
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bioRxiv - Neuroscience 2024Quote: ... mutations (Figure 5I) were introduced using the Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... N2-A225 and I256-A2731 were deleted using the NEBuilder HiFi DNA assembly master mix (New England Biolabs), respectively.
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bioRxiv - Neuroscience 2024Quote: ... 6 μl of 10 mM MnCl and 6 μl (=2400 units) of Lambda phosphatase (#P0753, NEB). Dephosphorylation was carried out at 30°C for 30 minutes and stopped by addition of Roti Load buffer (#K929.1 ...
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bioRxiv - Neuroscience 2024Quote: ... 4.1 μl of PLPPR3 ICD (final concentration 0.075 mg/ml) was mixed with 0.2 μl purified PKA catalytic subunit (final concentration 20 000 Units; #P6000S, Biolabs), 0.8 μl phosphorylation buffer (final concentration of 25 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... Single strand DNA oligonucleotides were ligated using T4 Polynucleuotide Kinase (New England Biolabs) using manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Ligated oligonucleotides were then assembled with pLV-U6-sgRNA using BsmBI (New England Biolabs) and T7 ligase (Thermo) ...
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bioRxiv - Neuroscience 2024Quote: ... Stranded cDNA libraries were constructed using 200 ng of total RNA per sample and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Low quality DNA were then reconcentred with AMPure XP beads and libraries were constructed according to the NEBNext® Ultra II DNA Library Prep for Illumina® kit recommendations (New England Biolabs, Ipswich, MA, USA). Each library was individually labeled and subjected to 12 PCR cycles for final enrichment and pooled in equal quantities ...
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bioRxiv - Developmental Biology 2024Quote: ... Luna Universal qPCR Master Mix (NEB) was used to amplify 2.0 microliters of each cDNA sample in a 20 microliter total reaction using the standard protocol on an Applied Biosystems StepOne Real-Time PCR system (Thermo) ...
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bioRxiv - Developmental Biology 2024Quote: ... Stored lenses were kept at either 4°C for up to one week or -20°C for up to one month before purification of total RNA with the Monarch Total RNA Miniprep Kit (NEB). We used approximately 30 lenses at 10 dpf ...
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bioRxiv - Developmental Biology 2024Quote: ... Strand-specific libraries were generated from the rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... Poly(A) mRNA library preparation was performed using NEBNext Ultra II DNA library prep kit for illumina (New England BioLabs) and 2x100bp paired-end sequencing was performed at a depth of 50 million reads on an Illumina Novaseq 6000 platform by the University of Toronto Donelly Sequencing Centre ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: The sequencing library was constructed using the NEBNext Ultra II FS Kit (New England Biolabs) according to the prime-seq protocol ...
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bioRxiv - Developmental Biology 2024Quote: A Primer Exchange Reaction concatemerization reaction (1X PBS; 10 mM MgSO4; dNTP, 0.6 mM of A, C, and T, NEB-N0446S ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.6 mM of A, C, and T, NEB-N0446S; 0.1 µM Clean.G; 800 U/ml Bst Polymerase, NEB-M0374L ...
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bioRxiv - Developmental Biology 2024Quote: ... Final constructs were assembled by Gibson assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Beads were resuspended in 63 μl Elution Buffer (NEB) and incubated at 34°C for 30 minutes prior to sample elution ...
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bioRxiv - Developmental Biology 2024Quote: ... bulk-extracted genomic DNA from these strains was used to set up PCR reactions for each primer set using Quick Load Taq 2X Master Mix (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were diluted in 90 μl Elution Buffer (NEB), mixed with 120 μl AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: ... gel purified and transcribed with DIG RNA labeling mix with either T7 RNA polymerase (NEB, M0251S; antisense probe), or SP6 RNA polymerase (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Q5 high fidelity DNA polymerase (NEB), PrimeStar Max (Takara ...
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bioRxiv - Developmental Biology 2024Quote: ... We verified that this guide RNA digested the Gbait plasmid in combination with Lba Cas12a (New England Biolabs) in an in vitro reaction (data not shown) ...
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bioRxiv - Developmental Biology 2024Quote: ... one cell stage zebrafish embryos were injected with 50 nM EnGen® Lba Cas12a (Cpf1) (New England Biolabs) and each gRNA at a final concentration of 2 nM ...
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bioRxiv - Developmental Biology 2024Quote: ... the Gbait and dld gRNAs were combined at 4.8uM each with 20uM Lba Cas12a protein (New England Biolabs) and incubated for 10 minutes at 370C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and sequenced on an Illumina Hi-Seq 2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digest assay with BtsCI (New England Biolabs) was performed to identify WT ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 μl 10X NEBuffer 2 (New England Biolabs) and nuclease-free water (total of 20 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... Rehybridized products were incubated with 1.5 μl T7 endonuclease I enzyme (New England Biolabs) at 37°C for 1 h and separated on 1.5% agarose gel.
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bioRxiv - Developmental Biology 2024Quote: ... The cDNA sequence for dld was cloned from isolated cDNA using Phusion High Fidelity Polymerase (New England Biolabs) and cloned into a pME vector digested with EcoRI using Gibson Assembly and the following primers for homology ...
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bioRxiv - Developmental Biology 2024Quote: ... the regulatory region of each gene was inserted into SmaI digested pUC19 together with GFP amplified from pPD95.75 using NEBuilder® HiFi DNA Assembly Master Mix (NEB - E2621). PCR products or Plasmids were micro-injected into CB4088 [him-5(e1490)] hermaphrodites with myo-2::mCherry from plasmid pCFJ90 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... following vector digestion with BamHI-HF and SpeI-HF restriction enzymes (New England Biolabs). To create pAK1440 (mamG-GFP) ...
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bioRxiv - Cell Biology 2024Quote: ... following vector digestion with BamHI-HF and EcoRI-HF restriction enzymes (New England Biolabs). To create pAK1447 (GFP-mms6NTDmmsF) ...
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bioRxiv - Cell Biology 2024Quote: ... following vector digestion with BamHI-HF and EcoRI-HF restriction enzymes (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... and the methylation efficiency verified by the CpG methylation-sensitive restriction enzyme BstUI (restriction site: CpG↓CpG, #R0518S, New England Biolabs, USA). The insert DNA fragments with or without methylation were ligated with the same double-digested splicing reporter vector DUP17512 ...