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Citations for New England Biolabs :
1651 - 1700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... RNA-seq transcriptome libraries were prepared following the NEB Next Ultra RNA Library Prep Kit from Illumina (NEB). NEB Next Poly(A ...
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bioRxiv - Bioengineering 2024Quote: ... coli Shuffle T7 strain (NEB) was transformed with pET17b OVA-ZE ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs), validated on TapeStation (Agilent Technologies) ...
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bioRxiv - Biophysics 2024Quote: ... plasmids purified as described above harboring the region of interest were digested by BsmI (NEB, Ipswich MA). The reaction mixture was prepared in a nuclease-free microcentrifuge tube ...
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bioRxiv - Biophysics 2024Quote: ... coli BL21(DE3)T1R (New England Biolabs) was used to express full-length NadA variant 3 following transformation with pET21-NadA ...
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bioRxiv - Developmental Biology 2024Quote: ... and 6 U SalI (New England Biolabs, Cat. No. R0138S)) were incubated with the following condition ...
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bioRxiv - Developmental Biology 2024Quote: ... Reagents (400ng of pT7-gRNA, 0.25 µM annealed oligo, 1x NEBuffer 3.1 (New England Biolabs, Cat. No. B7203), 1x T4 DNA ligase buffer (Promega Co. ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 U BglII (New England Biolabs, Cat. No. R0144S) and 6 U SalI (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 U BsmBI (New England Biolabs, Cat. No. R0580), 3 U BglII (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... multiplexed stranded cDNA sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) using poly(A ...
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bioRxiv - Immunology 2024Quote: ... Oxford Genomics Centre prepared bulk RNA libraries using an NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced the samples at a depth of 25 million reads per sample on a NovaSeq6000 (Illumina) ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Pathology 2024Quote: ... Prior to transformation the bait plasmids were linearised in the URA3 gene by PCR or restriction digest (BbsI/Esp3I/BstBI (NEB)) to allow integration into the genome ...
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bioRxiv - Pathology 2024Quote: ... schachtii gDNA (Q5 polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The isolated RNA was then reverse transcribed into first-strand cDNA using LunaScript RT SuperMix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... qRT PCR was performed with the LUNA Universal qPCR Master Mix (NEB) following manufacturer’s instructions and 1 µl of cDNA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2.6 µL of 10% NP-40 (Cat.#B2704S; New England Biolabs), and 1.5 µL of PNGase F in a total reaction volume of 26.7 µL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 – 50 µg of protein lysate from each sample was denatured in 1X Glycoprotein Denaturing Buffer (Cat.#B1704S; New England Biolabs) with 20 µL total volume at 100°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or PNGase F (Cat.#P0708L; New England Biolabs). For Endo Hf digestion ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.6 µL of 10X GlycoBuffer 2 (Cat.#B3704S; New England Biolabs), 2.6 µL of 10% NP-40 (Cat.#B2704S ...
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bioRxiv - Physiology 2024Quote: ... Double stranded RNA was synthesized by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Whitby, ON, Canada) following manufacturers recommendations ...
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bioRxiv - Physiology 2024Quote: ... Samples were then thawed at room temperature and total RNA was isolated using the Monarch Total RNA Miniprep Kit following manufacturers protocol with an on-column DNase treatment to remove genomic DNA (New England Biolabs, Whitby, ON, Canada). Purified total RNA samples were subsequently aliquoted onto a Take3 micro-volume plate and quantified on a Synergy Multi-Mode Microplate Reader (BioTek ...
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bioRxiv - Physiology 2024Quote: ... After the PCR 20ul of the PCR product were restriction digested with BamH1 (NEB #xxx) for 1 hr at 37C ...
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bioRxiv - Plant Biology 2024Quote: ... and first strand cDNA was synthesized with One-Taq RT-PCR kit (New England Biolabs), according to manufacturer’s protocols ...
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bioRxiv - Physiology 2024Quote: RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s polyA mRNA workflow at the Advanced Analysis Center at the University of Guelph (Guelph ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit according to the manufacturer’s recommendation for Illumina® (NEB, USA). The library quality was assessed using the Agilent 2100 Bioanalyzer system ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmids were transformed into DH5a competent cells (New England Biolabs) on LB agar plates with ampicillin ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 500ng RNA using a Multiscribe High Capacity cDNA Synthesis kit (Thermo) and qPCR was performed using Luna qPCR Master Mix (New England Biolabs) against primers listed in table 2.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were barcoded using Illumina i5 and i7 adaptors from NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) kit (New England Biolabs) and sequenced using a 150-cycle NextSeq 500/550 High Output Kit v2.5 with 75 forward and 75 reverse reads ...
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bioRxiv - Molecular Biology 2024Quote: PCR was performed using Q5 High Fidelity 2X Master Mix (New England Biolabs, M0492) with the following temperature and times.
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bioRxiv - Molecular Biology 2024Quote: ... whose cDNA was assembled from three fragments using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621S). A TAF4 G/C- rich fragment was synthesized as gene block with reduced G/C content (IDT).
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bioRxiv - Molecular Biology 2024Quote: ... DNA was converted using the Enzymatic Methyl-seq conversion module (NEB) following manufacturer’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... and EcoRI-HF (NEB) restriction enzymes according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Each synthetic DNA fragment was digested by SfiI (New England Biolabs) and ligated into SfiI digested backbone from pCH117 using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... with the primers constructed using the NEBuilder tool (NEB). Primers used for site-directed mutagenesis constructs are found in Table.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were amplified following heat-shock transformation of NEB-5alpha competent cells (#C2992H, BioLabs), and purified with the NucleoBondXtra Maxi Plus kit (#740416 ...
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bioRxiv - Microbiology 2024Quote: ... primers were designed to clone Ceg10 into pET28b using restriction enzymes NdeI and NotI by HiFi DNA Assemby kit (NEB; Table S4), producing the His6x-TEV-Ceg10 vector ...
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bioRxiv - Microbiology 2024Quote: ... Purified amplicons were cloned into STARR-seq luciferase reporter vector within the XbaI and FseI sites using Gibson assembly master mix (NEB Cat. No. E5510). The STARR-seq luciferase reporter (vector_ORI_empty ...
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bioRxiv - Microbiology 2024Quote: ... into BamHI and SalI sites of Addgene #99297 plasmid using T4 DNA ligase (NEB, Cat. No. M0202).
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bioRxiv - Molecular Biology 2024Quote: ... Samples were mixed 1:1 with 2x RNA dye (NEB B0363S), loaded on TBE gels ...
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bioRxiv - Neuroscience 2024Quote: ... pReC-hPINK1R145Q and pReC-hPINK1L347P were created by using Q5® Site-Directed Mutagenesis (NEB) with primers F_mut_PINK1_L347P ...
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bioRxiv - Neuroscience 2024Quote: ... pReC2-hRab39T168K and pReC2-hRab39G192R were created by using Q5® Site-Directed Mutagenesis (NEB) with primers F_mut_hRab39_T168K ...
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bioRxiv - Neuroscience 2024Quote: ... To clone inserts into plasmids NEBuilder® HiFi DNA Assembly (NEB) was used.
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bioRxiv - Molecular Biology 2024Quote: ... The following antisense oligonucleotides were 32P-labelled with T4 PNK (NEB) and used as probes ...
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bioRxiv - Neuroscience 2024Quote: ... Phusion High-Fidelity DNA Polymerase (cat# M0530L, New England Biolabs, MA, USA), and Sanger sequencing at Massey Genome Service ...
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bioRxiv - Microbiology 2024Quote: ... 5’-GCT GCG TTC TTC ATC GAT GC-3’) were chosen with barcodes for PCR amplification using Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA). The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... the only exception is the strain NEB5alpha (NEB reference #c2992) used for cloning purposes ...
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bioRxiv - Microbiology 2024Quote: ... Flanking regions of the target genes were amplified via PCR using Phusion polymerase (New England BioLabs). All primers used in the study are listed in Table S7.
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...