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Citations for New England Biolabs :
1551 - 1600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... or AsiSI (NEB, Cat.R0630L) or PmeI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 5.5 mL of Rnase A (NEB, Cat. T3018L) and 5.5 mL of RNase If (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng of a 1kB Plus DNA ladder (NEB, Cat.N3200) was included as a size reference ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 1 μg of genomic DNA was restricted with HinfI (NEB, Cat.R0155M) and RsaI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... an optional PBS wash followed by Red Blood Cell lysis step was included (10 mins at RT) prior to hypotonic lysis with Nuclei Prep Buffer (NEB, Cat.T3052) and Rnase digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5.5 mL of RNase If (NEB, Cat. M0243L) per million cells for 15 seconds and mixed by flicking ...
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bioRxiv - Molecular Biology 2024Quote: TeloTags were prepared in 100μL reactions with 5mM of each of the 6 permutations of telomere splint Extended Data Fig.1A) and 30 mM of biotinylated adapter in HiFi Taq DNA Ligase Reaction Buffer (NEB, Cat. M0647S). Annealing was done by heating to 99 degrees and slowly decreasing the temperature 1°C /min in a Veriti™ 96-Well Thermal Cycler (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 40 μg of gDNA was incubated with 3μl of ClaI (NEB, Cat. R0197S) or AsiSI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... fresh or frozen cell pellets were osmotically lysed in presence of 150mL of Nuclei Prep Buffer (NEB, Cat.T3052) supplemented with 5.5 mL of Rnase A (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... PacI (NEB, Cat. R0547L) or EcoRI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated using a Monarch Total RNA Miniprep Kit (NEB), RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... The UbC-Fluc2-SV40 pA cassette was synthesized as DNA gBlocks (Integrated DNA Technologies, IDT) and inserted by Gibson assembly using NEBuilder HiFi assembly mix (New England Biolabs). Unless otherwise noted ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB), and then analyzed with QuantStudio Design & Analysis Software v.1.5.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were cloned into the NdeI-BamHI sites of pGBKT7 or pGADT7 vectors using NEBuilder (New England BioLabs, USA).
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of PvuI (NEB, Cat. R3150S) PacI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... or EcoRI (NEB, Cat.R0101M), and incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and assembled using Gibson Assembly Cloning Kit (NEB, E5510S) according to manufacturer instructions ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and mRNA was isolated and purified using the Magnetic mRNA Isolation Kit (New England BioLabs). Resulting purified mRNA was quantified using Nanodrop.
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bioRxiv - Immunology 2024Quote: ... Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). FLAG-tagged cDNAs were transferred into pTrip-CMV-Puro-T2A-RFP (72 ...
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bioRxiv - Molecular Biology 2024Quote: ... Annealed products were ligated to the cleaved px330 plasmid using T4 DNA ligase (NEB, M0202T) and specific buffer (NEB ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and total RNA was isolated and purified using the Monarch Total RNA Miniprep Kit (New England BioLabs). This purified RNA was then used to prepare sequencing libraries with the Tru-Seq Stranded with RiboZero Gold (Human/Mouse/Rat ...
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bioRxiv - Molecular Biology 2024Quote: ... gRNA-Cas9 plasmids were generated upon cleavage with BbsI restriction enzyme (NEB, R3539M) of the px330 plasmid following NEB instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... and specific buffer (NEB, B0202S) following manufacturer instructions ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were incubated with 1:50 Endonuclease V (New England BioLabs) in a calcium containing blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 µL of Rapid PNGase F (New England Biolabs, Fig. 2d and Extended Data Fig. 2a) were added with 1.5 µL of 10× PNGase F buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Patient mutations were introduced using Q5 site-directed mutagenesis Kit (NEB). Transduction was performed as described previously (50).
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bioRxiv - Molecular Biology 2024Quote: ... ABCA4 missense variants were introduced in WT-ABCA4-SmBiT by site-directed mutagenesis using Q5 Site-directed mutagenesis kit quick protocol (NEB, E0554) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... coli cells (#C2988, NEB) or NEB 5-alpha Competent E.coli (High Efficiency ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of XBA1 (NEB), 1.5□l of 100mM spermidine and the reaction was made up to 150□l with nuclease free water ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were pre-treated with exogenous RNase H (M0297, NEB), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNase I (NEB, M0303L) was added to the reaction system for further incubation at 37 °C for 30 mins to eliminate the DNA template ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM NTPs (NEB, N0416S) and DNA template ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5□l of 10m/ml BSA (NEB), 15□l of 2.1 buffer (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 15□l of 2.1 buffer (NEB), 30 units of SSP1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... an equal amount of DNA (8μg) was treated with 8□l of RNase H in 1x RNase H buffer (NEB) for 5 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of SSP1 (NEB), 30 units of BSRG1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of BSRG1 (NEB), 30 units of ECoR1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 U/μL T7 RNA polymerase (NEB, M0251L), 1 U/μL RNase inhibitor (NEB ...
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bioRxiv - Immunology 2024Quote: ... To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs, NEB) overnight at 55 °C ...
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bioRxiv - Immunology 2024Quote: ... To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs, NEB) overnight at 55 °C ...
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bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... in 2X NEBNext® Ultra™ II Q5® Master Mix (NEB) subjected to the following reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 U/μL RNase inhibitor (NEB, M0314S), 1 mM NTPs (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of HindIII (NEB), 30 units of XBA1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of ECoR1 (NEB), 30 units of HindIII (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... cerevisiae REV7 gene was amplified from genomic DNA by PCR using the primer pair (forward OSB01 and reverse OSB02) and Phusion DNA polymerase (NEB, Ipswich, MA) as described (Sambrook and Russell ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM ribonucleoside-vanadyl complex (New England Biolabs, # S1402S)) ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a post-PCR cleanup kit (New England Biolabs) per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... we amplified the targeted gene regions by PCR (NEB Next High-Fidelity 2xPCR Master Mix ...