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Citations for New England Biolabs :
1501 - 1550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and Illumina sequencing adaptor ligation (New England BioLabs, E6000B- 10). Libraries were then indexed and PCR amplified (10 cycles ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and BglII (NEB, R0144L). Linker oligonucleotides ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (10 µg) was digested overnight at 37°C with 100 U each of the restriction endonucleases MseI (NEB, R0525L) and BglII (NEB ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and 800 U T4 DNA ligase (NEB, M0202S). Viral LTR–host DNA junctions were amplified using semi-nested PCR with a unique linker-specific primer and LTR primers ...
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bioRxiv - Molecular Biology 2024Quote: Genomic phage DNA was digested using either a combined treatment of DNaseI and ExoI to create single nucleotides or a commercially available nucleoside digestion mix (NEB, Ipswich, USA).
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of XBA1 (NEB), 1.5□l of 100mM spermidine and the reaction was made up to 150□l with nuclease free water ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were pre-treated with exogenous RNase H (M0297, NEB), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNase I (NEB, M0303L) was added to the reaction system for further incubation at 37 °C for 30 mins to eliminate the DNA template ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM NTPs (NEB, N0416S) and DNA template ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5□l of 10m/ml BSA (NEB), 15□l of 2.1 buffer (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 15□l of 2.1 buffer (NEB), 30 units of SSP1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... an equal amount of DNA (8μg) was treated with 8□l of RNase H in 1x RNase H buffer (NEB) for 5 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of SSP1 (NEB), 30 units of BSRG1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of BSRG1 (NEB), 30 units of ECoR1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 U/μL T7 RNA polymerase (NEB, M0251L), 1 U/μL RNase inhibitor (NEB ...
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bioRxiv - Immunology 2024Quote: ... To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs, NEB) overnight at 55 °C ...
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bioRxiv - Immunology 2024Quote: ... To subclone this pool we digested our protospacer-empty Libr.1-null transfer vector with BsmBI-v2 (New England Biolabs, NEB) overnight at 55 °C ...
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bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... in 2X NEBNext® Ultra™ II Q5® Master Mix (NEB) subjected to the following reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 U/μL RNase inhibitor (NEB, M0314S), 1 mM NTPs (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of HindIII (NEB), 30 units of XBA1 (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 units of ECoR1 (NEB), 30 units of HindIII (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... cerevisiae REV7 gene was amplified from genomic DNA by PCR using the primer pair (forward OSB01 and reverse OSB02) and Phusion DNA polymerase (NEB, Ipswich, MA) as described (Sambrook and Russell ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM ribonucleoside-vanadyl complex (New England Biolabs, # S1402S)) ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a post-PCR cleanup kit (New England Biolabs) per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... we amplified the targeted gene regions by PCR (NEB Next High-Fidelity 2xPCR Master Mix ...
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bioRxiv - Immunology 2024Quote: ... we amplified the targeted gene regions by PCR (NEB Next High-Fidelity 2xPCR Master Mix; New England Biolabs) using primers (Supplementary Table 2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The DNA concentration was determined using NEBnext library quant kit (NEB) and capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Variants were transformed into BL21(DE3) cells (NEB), a single clone was picked and grown to saturation in LB media supplemented with kanamycin (50 µg/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... Restriction digest with AclI (NEB) and Bsu36I (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... The product was subjected to DpnI digest (NEB) overnight and purified using SPRIselect beads (Beckman coulter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Upon preparation of the PCR mix (NEBNext Q5 mix, NEB) following the instructions of the manufacturer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The NEBuilder HiFi DNA Assembly kit (NEB) was used to create the plasmid EPG-IRES-GCaMP6m ...
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bioRxiv - Plant Biology 2024Quote: ... Using NEBNext Ultra II kits for Illumina (New England BioLabs, Ipswich, MA), each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode (for removal of PCR duplicates ...
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bioRxiv - Plant Biology 2024Quote: ... The beads were then boiled in SDS-loading buffer for subsequent western blotting using MBP antibodies (NEB, E8032S). Histone peptides utilized in this study were synthesized by EpiCypher and included H3 (1–32 aa) ...
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bioRxiv - Plant Biology 2024Quote: ... The NEBNext Ultra RNA Library Prep Kit (NEB, USA, Cat. #E7530L) was used according to the manufacturer’s recommendations and index codes were incorporated into the libraries to attribute sequences to their respective samples ...
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bioRxiv - Plant Biology 2024Quote: ... PCR using Q5 High-Fidelity DNA polymerase (NEB), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from agar plate-grown seedlings by acid guanidinium thiocyanate-phenol-chloroform-based extraction and purified from the aqueous phase using the Monarch RNA Clean Up Kit (New England Biolabs, Ipswich, MA, USA; NEB). Genomic DNA in the samples was removed using TURBO™ DNase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from agar plate-grown seedlings by acid guanidinium thiocyanate-phenol-chloroform-based extraction and purified from the aqueous phase using the Monarch RNA Clean Up Kit (New England Biolabs, Ipswich, MA, USA; NEB). Genomic DNA in the samples was removed using TURBO™ DNase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... each genomic segment was amplified as two PCR products and cloned in the pDIVA backbone (Wetzel et al., 2018) using the NEBuilder HiFi DNA Assembly kit (NEB). The CP-RT ORF (without viral UTRs ...
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bioRxiv - Neuroscience 2024Quote: ... An imaging activation mix was prepared by adding RNAse inhibitor (100 μL, New England BioLabs) to 250 μL Imaging Buffer Activator (VIZGEN ...
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bioRxiv - Plant Biology 2024Quote: ... 75 roots were ground to a powder using a cooled pestle and mortar and homogenized in 20 ml Nuclei Extraction buffer (NEB) (20 mM MOPS (pH 7) ...
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bioRxiv - Plant Biology 2024Quote: ... All clones were propagated in NEB10β Competent cells (NEB C3019I), and confirmed via Sanger (Eurofins Genomics ...
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bioRxiv - Neuroscience 2024Quote: Total RYR1 transcripts were amplified as 7 overlapping PCR products.38 They were sequenced after fragmentation and library preparation using NEBNEXT NGS workflow (New England Biolabs) according to manufacturer recommendation ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560S) and 18-mer oligo dT primers ...
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bioRxiv - Neuroscience 2024Quote: ... the resulting product was purified via column purification and underwent further amplification and conversion to RNA by a high-yield in-vitro transcription reaction using T7 polymerase (NEB, E2040S). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from a pENTR cyfip2-EGFP plasmid [32] using custom primers and the Q5 Site Directed Mutagenesis Kit (NEB) to induce the desired C179R (ΔRac1 ...
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bioRxiv - Neuroscience 2024Quote: ... purified ∼25−50-nt RNA fragments underwent treatment with recombinant shrimp alkaline phosphatase (New England Biolabs; M0371) to remove 5’-and 3’-phosphates ...