Labshake search
Citations for New England Biolabs :
1251 - 1300 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
-
bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
-
bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
-
TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Chromatin was pre-cleared with 20 μl Protein A magnetic beads (New England Biolabs, S1425S) for 1.5 h and 100 μL of the solution was saved as an input control ...
-
bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
-
bioRxiv - Developmental Biology 2023Quote: ... The protein products were purified using the NEBExpress Ni Spin Columns (New England Biolabs, S1427S). The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: RNA-protein complexes were isolated with oligo[dT)]25 magnetic beads (New England Biolabs, S1419S). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... For embryo microinjection the annealed sgRNA were each complexed with Cas9 protein (New England Biolabs) at room temperature for 10min in injection buffer (final concentrations ...
-
bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
-
bioRxiv - Bioengineering 2023Quote: ... The luciferase protein expressed in NiCo21(DE3) competent Escherichia coli (New England Biolabs, MA, USA) was purified using a Ni-NTA agarose resin column (Qiagen ...
-
bioRxiv - Cell Biology 2023Quote: ... All aliquots were supplemented with 10X NEBuffer for Protein MetalloPhosphatases (PMP) and 10X MnCl2 (NEB). The first aliquot (Untreated control ...
-
bioRxiv - Biochemistry 2023Quote: ... protein was combined with 1.5x molar excess of SNAP-Surface Alexa488 dye (NEB, Cat# S9129S). SNAP dye labeling was performed in buffer containing 20 mM Tris [pH 8.0] ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 1x NEBuffer for Protein MetalloPhosphatases (New England BioLabs; supplied with λPP), with 0.5% NP-40 and 0.01 mg/mL leupeptin ...
-
bioRxiv - Biochemistry 2024Quote: All protein mutagenesis was performed with the Q5 site-directed mutagenesis kit from NEB (E0554). Primers were designed with the NEBaseChanger tool (https://nebasechanger.neb.com/ ...
-
bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
-
bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
-
bioRxiv - Developmental Biology 2024Quote: ... adrb1-/- zebrafish was obtained by coninjection of Cas9 protein (EnGen Spy Cas9 NLS, M0646, NEB) with gRNA targeting adrb1 ...
-
bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
-
bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: PME measurements were carried out using a transient expression vector we created by inserting a synthetic gene block encoding the cDNA sequence of the human β2AR (Integrated DNA Technologies, Coralville, IA) into a modified pcDNA5 FRT using the NEBuilder kit (New England Biolabs, Ipswitch, MA). The final construct was designed to generate β2AR bearing an N-terminal influenza hemagglutinin tag from a transcript that also features a downstream internal ribosome entry site (IRES)-dasher GFP cassette that facilitates the unambiguous identification of positively-transfected cells ...
-
bioRxiv - Neuroscience 2020Quote: ... Methylation standards (100 % and 0 %) were either prepared by in vitro methylation of human genomic DNA using M.SssI CpG methyltransferase (NEB, Frankfurt a.M; Germany) or by whole-genome amplification using the REPLI-g Mini Kit (Qiagen ...
-
bioRxiv - Cell Biology 2021Quote: ... plasmid encoding GFP-labeled dominant negative mutant version of human Rab5B isoform was introduced to plasmid #1008 using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions using forward primer #1554 (AGTGGGAAAGaacAGCCTGGTATTAC ...
-
bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
-
bioRxiv - Molecular Biology 2022Quote: ... The G4 sequence and larger 5′ UTR (three human β-globin 5′ UTR sequences in tandem) were inserted using the Q5 Site-Directed Mutagenesis Kit (NEB # E0552S). pcDNA3.1(+)/mEGFP was a kind gift from Jeremy Wilusz (Baylor College of Medicine) ...
-
bioRxiv - Cancer Biology 2023Quote: ... library was prepared using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles ...
-
bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
-
bioRxiv - Biochemistry 2023Quote: ... An mRNA transcript library for Illumina sequencing was created using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA). A NextSeq 500 sequencer (Illumina ...
-
bioRxiv - Biochemistry 2023Quote: ... or deletion of the mitochondrial targeting sequence (dMTS) of the human FAM210A coding sequence (CDS; Uniprot Entry # Q96ND0) region was amplified using Q5 DNA polymerase (NEB, Cat # M0491). The FAM210A DNA was inserted into 2Cc-T and 2CT-10 vectors using the Ligation Independent Cloning (LIC ...
-
bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
-
bioRxiv - Cell Biology 2020Quote: ... S141A ABHD11 and H296A ABHD11 with C-terminal eGFP tags or HA tags were created using NEBuilder HiFi (NEB). ABHD11 was also cloned into a transfection vector ...
-
bioRxiv - Genomics 2020Quote: ... nuclei were centrifuged 5min at 3,000 rpm and ligation was performed 4h at 16°C using 10,000 cohesive end units of T4 DNA ligase (NEB) in 1.2ml of ligation buffer (120μl of 10X T4 DNA Ligase Buffer ...
-
bioRxiv - Microbiology 2020Quote: ... RNA and 3′ adapters were incubated at 22°C for 2.5 hr with 51 U of T4 RNA Ligase I (NEB) and 12 U of recombinant RNase inhibitor (Takara Bio ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and then a final extension at 72°C for 2 minutes using Q5 High-Fidelity Polymerase (New England BioLabs). We conducted a nested PCR to sequence exon 2 (204bp ...
-
bioRxiv - Microbiology 2021Quote: ... at 50°C for 60 min with a subsequent Klenow DNA polymerase step using 5 units (New England Biolabs) at 37°C for 60 min ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated for 30 min at 37°C in 100 μl A-tailing mix (1x NEB buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1µg purified RNA was incubated at 37°C for 30 min with 10 units of T4 PNK (NEB, M0201S) in PNK buffer containing 20 units SUPERase•In in a 25 µl reaction to remove 2′-3′-cyclic phosphates of reporter RNAs ...
-
bioRxiv - Genomics 2020Quote: Micro-C sequencing library was generated by using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) with some modifications ...
-
bioRxiv - Cell Biology 2019Quote: ... were modified based on the pcDNA3.1(+)-C-I30-FLG-TEV plasmid via the Q5 Site-Directed Mutagenesis Kit (cat#: E0554S, NEB). pCMV-FLAG-ABCE1 was a gift from Dr ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was applied to amylose resin (NEB). The column was washed with 10 column volumes (CV ...
-
bioRxiv - Genomics 2019Quote: ... fragments compatible with Illumina NGS were generated digesting the products of rolling circle amplification with BtsαI (55 °C, 90 min, New England Biolabs) and SalI-HF (37 °C ...
-
bioRxiv - Microbiology 2019Quote: ... of desired templates were in vitro-transcribed overnight at 37°C with 2.5 mM Ribonucleotide Solution Mix (New England Biolabs) and when needed ...
-
bioRxiv - Biochemistry 2020Quote: ... Vector and fragment were ligated in an overnight reaction at 4 °C using T4 DNA Ligase (New England Biolabs). After transformation ...
-
bioRxiv - Genomics 2021Quote: ... non integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 minutes heat inactivation at 65 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... Digested DNA was ligated during 5 h incubation at 16 °C with 100 U of T4 DNA ligase (NEB). DNA was recovered after reverse crosslinking and Proteinase K treatment (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... PCR conditions for ACMV were similar except the annealing temperature was 55°C and Hot Start Taq Polymerase (NEB optimized for large product sizes was used ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were then incubated at 65°C for 30 minutes in the presence of BsmI restriction enzyme (NEB). The resulting DNA fragments were separated by 1.5% agarose gel electrophoresis ...
-
bioRxiv - Genomics 2021Quote: ... 100 ng of genomic DNA were digested for 6h at 65°C with 20 U TaqI (New England Biolabs) and 6h hours at 37°C with 20 U of MspI (New England Biolabs ...
-
bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...