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Citations for New England Biolabs :
1201 - 1250 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... W165F and S169A) were introduced into human PSEN1 cDNA cloned into the pMSCVpuro vector using Q5 Site-Directed Mutagenesis Kit (NEB BioLabs) according to the standard protocol ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Developmental Biology 2023Quote: Human and bovine XIST E repeats were PCR amplified from cDNA generated from either total RNA of Ishikawa (human) or bovine stromal cells (Supplementary Table S4) with the Q5 High-Fidelity DNA polymerase (NEB, UK) or the non-proofreading Taq DNA Polymerase (EP040 ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 containing human RNaseH1 with D210N catalytic dead mutant was amplified by PCR for cloning in pEGFP-N1 using EcoRI (NEB, #R3101S) and KpnI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: The total RNA from the HG and LG treated βTC6 cells were rRNA depleted using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB), followed by fragmentation and the cDNA library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The mCherry gene was excised from pL1694 by BamHI and NotI and replaced by a human codon-optimised spCas9 from pUF1-Cas912 by Gibson cloning (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs, NEB) to generate pL1694-GIMO-Cas9.
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bioRxiv - Microbiology 2020Quote: Fetuin was deglycosylated under denaturing conditions using Protein Deglycosylation Mix II kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... GST fusion protein on glutathione beads was incubated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Association occurred in samples containing ACE2- fusion proteins either without or with TEV protease (NEB) treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs, #M0251S) by 10% SDS-PAGE gel electrophoresis stained with Coomassie blue (Bio-rad).
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bioRxiv - Molecular Biology 2021Quote: The hybrid protein and AβBP were cloned into pMAL-C5E MBP vector (New England Biolabs) with a cleavage site for 3C protease immediately after MBP ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNAs were then associated with EnGen® Spy Cas9 NLS protein (New England BioLabs) at room temperature for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... The bead-bound protein was incubated with 3 µM SNAP-Surface Alexa Fluor 647 (NEB) for 40-60 min at 4°C (to fluorescently label the protein) ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted proteins were incubated with TEV protease and MBP-fusion endoglycosidase (EndoHf, New England Biolabs) at 4 °C overnight ...
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bioRxiv - Genomics 2021Quote: - T4 gene 32 protein (10 mg/mL, NEB or EPFL “homemade” T4g32p, 10 mg/mL). In 96-well plates ...
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bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... 300 ng of gRNA transcript was bound to a purified Cas9 protein (New England Biolabs) for 10 minutes at 25° C ...
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bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for up to 5 μg trimer ...
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bioRxiv - Plant Biology 2020Quote: ... MBP and GST fusion proteins were separated from supernatants using amylose resin (New England Biolabs) and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... PhyR1 and PhyR2 proteins were overexpressed and purified using the IMPACT kit (New England Biolabs) following the manufacturer’s instructions and equal procedures for the four of them ...
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bioRxiv - Immunology 2020Quote: The I53-50A and I53-50B.4.PT1 proteins were expressed in Lemo21(DE3) (NEB) in LB (10 g Tryptone ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). For all proteins except PP1γ ...
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bioRxiv - Biophysics 2020Quote: ... The overhang DNA was added to the protein-oligo chimera together with T4 ligase (NEB) and incubated for 30 min at 16°C followed by 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... For embryo microinjection the annealed sgRNA were complexed with EnGen Cas9 protein (New England Biolabs) at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... C57BL/6NJ zygotes were co-injected with Cas9 protein (50 ng/μl) (New England Biolabs) or Cas9 mRNA (1000 ng/μl ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns of MBP-tagged fusion proteins were performed using an amylose resin (New England Biolabs). Appropriate amounts of (see Fig ...
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bioRxiv - Biochemistry 2021Quote: ... 1μL m6A antibody per sample was coupled to pre-washed Protein G Magnetic Beads (NEB) in 1x reaction buffer (150mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Immobilized proteins were deglycosylated with endoH in 0.5% SDS and 40mM DTT (New England Biolabs) overnight at 37°C according to the manufacturer.
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bioRxiv - Cell Biology 2022Quote: ... and either incubated with buffer control or lambda protein phosphatase (λ) (New England Biolabs; P0753) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... which was injected into one-cell state embryos together with Cas9 protein (New England Biolabs). Mutant animals were identified by PCR using the primers ...
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bioRxiv - Cell Biology 2019Quote: ... were precleared using 40μl of Protein-A Magnetic beads (New England Biolabs Cat No-S1425S) to remove non-specific components from the lysate ...
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bioRxiv - Molecular Biology 2019Quote: ... in vitro translation was performed using the PURExpress In vitro Protein Synthesis kit (NEB 6800) and the non-stop ribosome complex was purified as previously described in ref.38 ...
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bioRxiv - Microbiology 2021Quote: ... The protein expression level was evaluated in four T7 expression strains: T7 Express (C2566, NEB), T7 Express with LysY and lacIq (C3013 ...
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bioRxiv - Molecular Biology 2021Quote: ... IgG (Epredia™, NC748P) or 1X PBS was incubated with protein A beads (NEB, S1425S) with agitation for 1 hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... to visualize the bands alongside a color prestained protein broad range standard (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: Injection mixes for CRISPR mediated manipulations consisted of 650 ng/ul dCas9 protein (NEB, M0652) or Cas9 protein (NEB ...
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bioRxiv - Genetics 2019Quote: ... sgRNAs were mixed with Cas9 protein (gift from Darren Gilmour, M0646M NEB, or B25641 invitrogen) and co-injected into 1-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μg of microsomal proteins were treated with EndoH-Hf (New England Biolabs, Ipswich, MA) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were formed from sgRNA and recombinant Cas9 2NLS protein (New England Biolabs) mixed at a sgRNA to Cas9 ratio of 4.5 ...
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bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... The lambda protein phosphatase procedure was carried out following the manufacturer’s guidelines (New England Biolabs). The sample was incubated at 30°C for 2 hours ...
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bioRxiv - Biophysics 2022Quote: Plasmids expressing stdMCP-fusion proteins were generated using the NEBuilder HiFi DNA Assembly kit (NEB). To obtain plasmids carrying an stdGFP-tagged stdMCP gene ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Bioengineering 2023Quote: The heterologous protein MBPeGFP was purified using NEBExpress Ni spin columns (NEB, Ipswich, MA, USA) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...