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Citations for New England Biolabs :
51 - 100 of 9513 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Concentrations were measured by Qubit RNA HS assay kit and 750 ng of Dnase-treated RNA was used for rRNA depletion by NEB Next rRNA depletion kit (Human/Mouse/Rat) ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Biochemistry 2023Quote: A luminescence based translation inhibition assay was performed using an in-vitro translation PURExpress® Δ Ribosome Kit from (NEB) The constituents of the kit were incubated with 25 ng pMSR DNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... indel assay was performed by T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321) to detect how efficiently a given sgRNA produces indels at targeted genomic DNA locus ...
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bioRxiv - Genetics 2019Quote: T7 endonuclease assays (M0302L, NEB) were performed following the manufacturer instructions.
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bioRxiv - Developmental Biology 2020Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Genetics 2022Quote: ... using a T7E1 assay (NEB), and compared to the activity of Tet2 sgRNA that has been shown to modify the mouse genome efficiently95 ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Microbiology 2021Quote: ... 3 µl of extracted RNA was used in a TaqMan one-step qRT/PCR assay (Luna® Universal One-Step RT-qPCR Kit, NEB). TaqMan analysis was carried out with primer/probe combination described in Table 1 and analysis performed on the QuantStudio™ 6 Flex System (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...
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bioRxiv - Zoology 2020Quote: The 16S rRNA and gltA amplicons from PCR assays were cleaned with the ExoSAP-IT kit (E1050, New England Biolabs, US) and Sanger sequenced ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA obtained from the ChIP-assays was adaptor-ligated and amplified using the NEBNext multiplex oligo kit (New England BioLabs, E7335S) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from three pooled killing assay spots (as detailed in the previous section) utilizing the Monarch® Genomic DNA Purification Kit (NEB). Quantification of genomic DNA copies was targeted at the kdpAB gene and carried out with the Naica® Crystal Digital PCR System using Sapphire chips (Stilla Technologies).
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bioRxiv - Microbiology 2024Quote: ... Gaussia luciferase and Cypridina luciferase expression levels were measured with a Biolux Gaussia luciferase assay kit (New England Biolabs, USA; #E3300) and Biolux Cypridina luciferase assay kit (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... a dsDNA HS qubit assay and also by a NEBNext library quantitation Assay (New England Biolabs) before sequencing on Illumina’s HiSeq2500v4
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bioRxiv - Developmental Biology 2021Quote: ... The T7E1 assay (Cat#M0302, NEB) was then applied as in (Sato et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutants selected for cloning were also analysed via T7 endonuclease I-based (T7E1) heteroduplex assay according to the manufacturers protocol (EnGen® Mutation Detection Kit, NEB #E3321), and the length of digested and undigested fragments were visually compared by gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 fmol of DNA (determined following quantification with the Qubit™ dsDNA HS Assay Kit) was processed using the NEBNext Companion Module for ONT Ligation Sequencing (NEB, #E7180). Sequencing adapters were added using the Ligation Sequencing Kit (ONT ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Genetics 2022Quote: ... T7 Endonuclease I assay (T7EI; NEB M0302L) or restriction enzyme digestion using Mse I (NEB R0525L) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the T7E1 assay (NEB #E3321) to determine potential mutations for every single fly following the protocol in (Zhu et al. ...
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bioRxiv - Cell Biology 2023Quote: ... a T7 endonuclease I cutting assay (NEB) was used to identify the extent of insertions/deletions for each guide ...
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bioRxiv - Microbiology 2024Quote: ... and the PNGase F assay (New England Biolabs) was completed following the manufacturer’s instructions using 15 µg of protein ...
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bioRxiv - Molecular Biology 2023Quote: ... coli transposition assays were treated with AvrII (NEB) to reduce the proportion of contaminating pDonor ...
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bioRxiv - Genetics 2021Quote: ... followed by T7 Endonuclease I Assay (New England Biolabs). 30 PCR cycles were run in all samples ...
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bioRxiv - Neuroscience 2019Quote: ... Editing activity was validated by the T7E1 assay (NEB) in rat C6 glioma cells (ATCC) ...
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bioRxiv - Neuroscience 2019Quote: ... T7 Endonuclease I assay (M0302, New England Biolabs Inc.) and Sanger sequencing (GENEWIZ ...
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bioRxiv - Neuroscience 2020Quote: ... we performed a T7 endonuclease I (T7EI) assay (NEB) on approximately 10 F1 embryos from a clutch to confirm whether any mutations had occurred in the germline of the injected parent ...
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bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digest assay with BtsCI (New England Biolabs) was performed to identify WT ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the T7 Endonuclease I (T7EI) assay (NEB).
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bioRxiv - Genetics 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) assay to detect mutations ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) to detect indel mutations ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Developmental Biology 2022Quote: ... 5’-CTTTTGACATCCGCTTCTGC-3’ followed by a T7 endonuclease assay (NEB) to detect indels ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... All assays were done in Escherichia coli (strain: NEB Turbo) following the same plasmid protection assay described previously ...
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bioRxiv - Cell Biology 2019Quote: ... Deglycosylation assay using PNGase F (P0704S, New England BioLabs Inc.) was performed according to manufacturer’s protocol with the exception of incubating the reaction for 3 hours at 37°C followed by overnight at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Developmental Biology 2020Quote: ... Mutations were detected through a T7 endonuclease I assay (NEB)(76) ...
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bioRxiv - Bioengineering 2024Quote: DNA cutting assays were performed following New England Biolabs (NEB) method.39,40 Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... The assays are composed of 1X Isothermal Buffer (NEB #B0537), 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003) ...
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bioRxiv - Microbiology 2024Quote: Assays were performed using the PURExpress ΔRibosome (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... was confirmed using a Lambda phosphatase assay (New England BioLabs).
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bioRxiv - Neuroscience 2021Quote: ... cell viability was determined utilizing an XTT assay (New England Biolabs). Similar results were obtained utilizing calcium phosphate transfection methods ...