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Citations for New England Biolabs :
9851 - 9900 of 10000+ citations for Human Complement Component 1 Q Subcomponent Binding Protein HABP1 C1QBP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: RNA-seq libraries were generated following the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420S) protocol for cells ...
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bioRxiv - Genomics 2023Quote: ... The fragmentation pattern was assessed via agarose gel electrophoresis and 0.5-1 μg of fragmented gDNA was used to prepare sequencing libraries using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and P2 adaptor ligation steps were performed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). After size selection using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: RT-qPCR was carried out from the isolated RNA using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) following the manufacturer instructions ...
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bioRxiv - Physiology 2023Quote: ... The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Physiology 2023Quote: ... between 400 and 3000 pg of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760) and amplified using NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2 ...
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bioRxiv - Zoology 2023Quote: ... ACPR ECL2 and ACPR ECL3) were assembled into pBudCE 4.1 Gα15 using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Whitby, ON) following the protocol guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were generated from 500 ng of total RNA using the NEBnext Ultra II RNA Library Prep kit (New England Biolabs), following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... then ligated into linearized plasmid backbones using an NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs Cat. No. E5520S). Recombinant plasmids were transformed into E ...
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bioRxiv - Cancer Biology 2023Quote: Double-barcoded lentiviral vectors were generated from FU-CGW by sequentially inserting matched 10-nucleotide barcodes into the PacI site distal to the HIV FLAP using the Quick Ligation Kit (New England Biolabs) and PCR amplification of the WPRE sequence and barcode with insertion into the ClaI sites proximal to the 3’ LTR by HiFi DNA Assembly (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We prepared the sample libraries with NEBNext DNA Library Prep kit for Illumina according to the manufacturer’s protocol (New England Biolabs E7645). We sequenced the samples with the Illumina 4000 HiSeq 150PE ...
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bioRxiv - Microbiology 2023Quote: ... Secreted cypridina Luciferase (cLuc) activity from the internal control plasmid was similarly measured using the cypridina Luciferase kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... RNAseq libraries were constructed by the Transcriptional Regulation and Expression (TREx) Facility at Cornell University using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... DNA removal coupled with cDNA synthesis were conducted using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2023Quote: ... then used as template to generate cDNA using NEBNext Ultra RNA Library Prep Kit for Illumina (England Biolabs, Ipwich MA). The resulting cDNA assessed with a high-sensitivity Agilent 2100 bioanalyzer ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting 1556 bp fragment was inserted into the pET28c vector digested with EcoRI and NcoI using the NEBuilder HiFi Assembly kit (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA synthesis was carried out using NEB T7 High Yield RNA Synthesis kit (New England Biolabs France Genopole, Evry, France), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturers’ instructions and converted to cDNA using ProtoScript First Strand cDNA Synthesis Kit (#E6300 New England BioLabs). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... Each of the 11 genes were cloned into a pENTR-DTOPO vector and finally into a pIX-HALO expression vector using a Gateway cloning kit obtained from NEB and following the manufacturer’s protocol (Supplemental Datasets S9-S19) ...
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bioRxiv - Molecular Biology 2023Quote: ... and size selected by running samples on a 1.8% LMT agarose gel at 120 V for 25 minutes at 4°C and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Purified samples were carried forward for qPCR or library preparation ...
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bioRxiv - Cell Biology 2023Quote: ... double and triple N>G mutations were introduced in the parental constructs by using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturers protocols and using custom designed primer ...
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bioRxiv - Microbiology 2023Quote: ... and given barcode sequences using the NEBnext Ultra-directional RNA Library Kit for Illumina (New England Biolabs, Ipswitch, MA, USA) with NEBnext Multiplex Oligos for Illumina (Primer Set 1 ...
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bioRxiv - Microbiology 2022Quote: ... Illumina sequencing libraries for each genome were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) using the manufacturer’s protocol with DNA input concentrations of 5-15 ng/µL ...
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bioRxiv - Microbiology 2023Quote: ... Cells were centrifuged 7,000xg/2min pellet was washed in 1ml of PBS and then resuspended in 400 μl of the resuspension buffer (Monarch Miniprep kit – NEB) containing 50 μg of lysostaphin ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing library was generated using NEB Next® Ultra TM DNA Library Prep Kit for Illumina (NEB, USA, Catalog#: E7370L) following manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Molecular Biology 2023Quote: ... Nucleotide substitution or deletion mutations were designed on the NEBaseChanger online tool and TOPO-cloned using the Q5 site-directed mutagenesis kit (New England Biolabs) according to the standard protocol.
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bioRxiv - Molecular Biology 2023Quote: ... . Site-directed mutagenesis was performed on 10 ng pET28a(+)-HisPI31wt template with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The final cleaned-up Hi-C library was used as input material for Illumina sequencing library prep kit (NEB, E7805) with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA constructs were used as a template to in vitro transcribed RNA using T7 high yield RNA kit (New England Biolabs). The synthesized RNA was DNase treated (TURBODNase) ...
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bioRxiv - Biophysics 2023Quote: ... DNA fragments were generated by PCR amplification and then fused with the backbones using NEBuilder HiFi DNA assembly kit (New England Biolabs). Constructs were transformed and amplified in NEB 5-alpha Competent E ...
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bioRxiv - Immunology 2023Quote: ... pcDNA3.1-FLAG-ZBTB48 WT was used as template for the generation of the constructs with point mutations in the C-terminal arm using the primers in Table S6 and the Q5 Site-Directed Mutagenesis Kit (NEB). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... the pool was subjected to NEBNext FFPE DNA Repair and Ultra II End-prep kits (M6630 and E7546, New England Biolabs). After that ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were constructed using approximately 6 ng of total EV RNA as input material for the RNA sample preparations using the Small RNA Library Prep Set for Illumina kit (New England BioLabs) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... where the band shift due to the insertion of the BSR cassette was confirmed in the case of homologous recombination and purified using a PCR purification kit (NEB), in which the nucleotide sequence was determined (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... mutants of UFD1-His6 (deletion of amino acids 2-215) and NPL4 (T590L+F591V point mutations) 18 were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: PCR amplification was carried out using a Q5® High-Fidelity DNA Polymerase kit (#M0491L, New England Biolabs, Ipswich, MA) using the following protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions with UDP-MurNAc-80mer RNA contained 2 µg RNA and products were purified using the Monarch RNA Cleanup Kit (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... Then the sequencing library was generated by the NEBNext® Ultra II DNA Library Prep Kit (New England Biolabs; E7103S) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1 ...
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bioRxiv - Cell Biology 2023Quote: ... Both isoforms were cloned into a pcDNA 3.1 mammalian expression vector using an NEBuilder® HiFi DNA Assembly kit (cat # E5520S, New England Biolabs). We obtained human FAM161A cDNA in p3XFLAG-CMV-7 from Dr ...
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bioRxiv - Microbiology 2023Quote: ... All samples were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, #E7760L), with an initial amount of 100 ng total RNA in 12 μl ...
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bioRxiv - Biochemistry 2023Quote: The K164A mutant was made by site directed mutagenesis using wild type LhCE as template with the Q5 Site-Directed Mutagenesis Kit from NEB using following primers:
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was obtained by reverse transcription of 0.5 μg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, #E6560S). Three independent biological replicates were prepared for each genotype ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was pre-amplified with pooled barcoded primers before libraries were prepared with NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) using the AMPure XP reagent (AgenCourt Bioscience ...
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bioRxiv - Plant Biology 2023Quote: ... pENTR221-Scs6 was used as a template to generate Scs6S793F and Scs6H510V via PCR mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated according to the manufacturer’s instructions: polyA-selected RNA was isolated and libraries were prepared using the NEBNext kit (New England Biolabs, e7500s). Purified libraries were quantified on an Agilent Technologies 2200 TapeStation with a D1000 ScreenTape assay ...
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bioRxiv - Microbiology 2023Quote: ... The amount of RNA template was equalized for reverse transcription using the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs) and random hexamers ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... followed by first-strand cDNA synthesis with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligo for Illumina (New England Biolabs ...