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Citations for New England Biolabs :
901 - 950 of 5045 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl protein A magnetic beads S1425S (New England Biolabs; Ipswich, MA) were washed 3 times in PBS and incubated overnight with 2-3 µg of antibody directed against CISD34 at 4°C with gentle mix ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA was incubated at room temperature with Cas9 protein (NEB, Cat# M0646T). AB* embryos were injected at the one-cell stage with the sgRNA and Cas9 cocktail in a 1nL volume at 25pg sgRNA/nL ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were purified by NEBExpress Ni Spin Columns (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
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bioRxiv - Microbiology 2024Quote: ... MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... The diluted sample was treated with 2 µl Lambda protein phosphatase (NEB) and incubated in water bath at 30 ℃ for 4 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Genetics 2024Quote: ... and 400 units of Lambda protein phosphatase (λpp) (New England Biolabs, P0753S). The reactions were incubated at 30°C for 30 minutes and terminated by an equal volume of 2x Laemmli buffer.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Cell Biology 2020Quote: ... samples were cooled and then digested with DpnII for one hour at 37° C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′-GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were thawed at 37°C and mixed with 20 µL of a 500U DNaseI (NEB), 10X buffer and nuclease free water mixture and incubated at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... were incubated for 12 hours at 30°C with 7.5 Units of Phi29 polymerase (NEB M0269) in Phi29 NEB buffer 1X ...
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bioRxiv - Systems Biology 2019Quote: ... 30°C and the growth was continuously monitored online using Cell Growth Quantifier (Aquila Biolabs GmBH). Samples were withdrawn at regular intervals for analyzing the spent metabolites through High-Performance Liquid Chromatography (HPLC).
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bioRxiv - Microbiology 2019Quote: ... which was placed towards the centre of a C760 Innova – 70 °C freezer (New England Biolabs) for 1 h before being thawed in a 37 °C waterbath for approximately two min.
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bioRxiv - Genetics 2019Quote: The product was digested overnight at 37°C using 20 U PmlI (New England Biolabs, USA), 5 uL accompanying buffer ...
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bioRxiv - Microbiology 2019Quote: ... and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S). After transformation into chemically-competent Top10 E ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.6) overnight at 37 °C in the presence of non-tritiated SAM (New England Biolabs). Samples were resolved via SDS-PAGE ...
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bioRxiv - Genomics 2019Quote: ... Tagmented DNA was End-Repaired for 30 minutes at 37°C (5U Klenow Exo-(NEB #M2012S), 1 × NEB Buffer 2 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were stored at -20° C before PCR amplification of cDNA with Q5 (New England Biolabs) or SuperFiII (ThermoFischer).
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... after ligation overnight at 4°C with the T4 DNA ligase (New England Biolabs, Evry, France).The sequences of the cloned fragments in the resulting plasmids pYES2::MlpCSP1 ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were incubated overnight at 60 °C with either BstNI restriction enzyme (New England Biolabs) or a control buffer solution without enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs, UK). The ligation mix was transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... Samples were treated twice for 45 minutes at 37°C with DNase I (New England Biolabs) with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... Individual fragments were amplified at 60°C with Phusion® High-Fidelity Polymerase (New England Biolabs) using T ...
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bioRxiv - Physiology 2021Quote: ... The pNB1u vector was digested overnight at 37 °C with PacI and Nt.BbvCI (New England Biolabs) to generate 8-nt overhangs ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C) and the supernatant was added to 24 mL of amylose resin (NEB, Ipswich MA) pre-equilibrated with buffer C and nutated for 2 hr at 4°C ...
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bioRxiv - Genomics 2021Quote: ... followed by an overnight incubation at 16°C with 3 µL T4 DNA ligase (NEB, M0202). Samples were purified with phenol-chloroform and used as 3C templates for Taqman-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were treated with proteinase K at 37 °C for 10 min (New England Biolabs) and the RNA samples were purified by RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... samples were cooled and then digested with DpnII for one hour at 37°C (NEB, R0543). These ligated pools were then amplified using AdR_PCR oligonucleotides as primer (5′ -GGTCGCGGCCGAGGATC-3′ ...
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bioRxiv - Cell Biology 2020Quote: ... pFA6a-mto2S338N-C-KanMX6 was generated using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) in an assembly containing 5 fmol of each fragment ...
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bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid and insert were then ligated overnight at 16 °C (T4 DNA ligase, New England Biolabs). After transformation into chemically competent E.coli and subsequent plasmid purification ...
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bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs, cat#B0201S). Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... cells were digested for 2 hours at 37°C using 375U of MboI (NEB, Cat. #R0147). After biotin filling ...
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bioRxiv - Genomics 2024Quote: ... followed by proteinase K treatment (New England Biolabs, #8102S 20µg/µL, 1.5 h at 55°C). DNA was eluted in Milli Q water ...
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bioRxiv - Genomics 2024Quote: ... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
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bioRxiv - Microbiology 2023Quote: ... Vector and insert were ligated overnight at 16°C using T4 DNA ligase (New England Biolabs) and transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was then digested using DNase I for 2h at 37°C (New England Biolabs) to remove residual plasmid DNA and then purified using Monarch RNA cleanup kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the P1-adaptors and Sbf I-HF (New England Biolabs)-digested DNAs for 90 min at 37°C were ligated by T4 ligase (New England Biolabs). After DNA pooling ...
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bioRxiv - Cell Biology 2023Quote: ... 45-72°C for 30 seconds (depending on melting temperature of primer pairs determined by NEB Tm calculator https://tmcalculator.neb.com) ...
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bioRxiv - Cell Biology 2024Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology ...