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Citations for New England Biolabs :
901 - 950 of 3093 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli Top10 (NEB, Ipswich, US) and plated on LB plates (25 g/L Luria-Bertani agar ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21(DE3) strain (NEB) were used ...
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bioRxiv - Plant Biology 2023Quote: ... coli cells (New England BioLabs). Plasmid DNA was isolated using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Subcloning Efficiency) (NEB, #C2988J) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... coli NiCo21(DE3) cells (NEB) and the cells were grown in LB media supplemented with 50 µg/mL Kanamycin at 37°C until OD600 reached 0.8 ...
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bioRxiv - Bioengineering 2024Quote: ... coli cells (New England Biolabs), and grown in 5 ml culture overnight at 37C ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DNA Polymerase I (NEB) and incubated the mix for 3 hours at 20□°C ...
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bioRxiv - Biochemistry 2023Quote: ... coli DH5a (New England Biolabs) was used for routine cloning and pathway propagation ...
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bioRxiv - Microbiology 2024Quote: ... 5α Competent Escherichia coli (NEB) cultured in lysogeny broth was used for plasmid preparation.
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bioRxiv - Cell Biology 2024Quote: ... coli RNase HII (NEB #M0288). Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml ...
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bioRxiv - Biophysics 2024Quote: ... coli cells (New Englad Biolabs) and purified with QIAprep spin miniprep kits (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... coli (New England Biolabs; C3040) made using the Mix&Go transformation kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB 5-alpha (NEB) cells stored at −80 °C were thawed on ice and transformed with the ligation mixture according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (C2987I, New England Biolabs). Positive colonies were screened by PCR using GoTaq G2 Hot Start green Master Mix (#M7422 ...
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bioRxiv - Neuroscience 2024Quote: ... coli Poly (A) Polymerase (NEB). sgRNAs of waca or wacb were injected with Cas9 mRNAs into 1-cell stage embryos.
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bioRxiv - Biochemistry 2024Quote: ... coli polyA polymerase (NEB, M0276L), desalted on P30 columns ...
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bioRxiv - Biophysics 2024Quote: ... coli 10-beta cells (NEB). Instead ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli poly(A) polymerase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: Escherichia coli strain K12 (NEB Turbo Competent E ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB5α (New England Biolabs) prior to transformation into the appropriate electrocompetent strains ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5lll (New England Biolabs) following the manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... coli (#C3040H, New England Biolabs) were transformed with the generated constructs ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (New England Biolabs). Recombinant expression for assessment of BcsF roles in macrocomplex assembly was carried out in a T1 phage-resistant 11bcs BL21*(DE3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (High Efficiency) (#C3019H, NEB) or Electroporation Protocol (#C3020 ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described22,33,52 ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described 44-46 ...
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bioRxiv - Microbiology 2021Quote: The CV-B3 Δ116-2C protein used for crystallogenesis was produced in Escherichia coli T7 Iq Express pLysS (New England BioLabs). Cells were grown in TB medium containing 100 µg/mL of ampicillin and 34 µg/mL chloramphenicol at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin proteins were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described [40–42] ...
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bioRxiv - Plant Biology 2024Quote: ... MBP-RVE4 and MBP-RVE8 proteins were expressed in Escherichia coli BL21 strain according to the manufacturer’s instructions using the pMAL Protein Fusion and Purification System (New England Biolabs; #E8200) and purified using MBPtrap HP column (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-SNAP-tag (New England Biolabs, #P9310S) and anti-Cyclin D1 (BD Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... or SNAP-tag (New England BioLabs, N9181S) were inserted into the retroviral plasmids pMRX-IP (harboring a puromycin-resistant marker ...
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bioRxiv - Genetics 2020Quote: ... anti-MYC-tag (Cell Signaling Technology/NEB) at 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tag DNA (pSNAPf, New England Biolabs) and β-Actin DNA (Actin mRFP-PAGFP was a gift from Guillaume Charras & Tim Mitchison ...
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bioRxiv - Microbiology 2024Quote: ... the SNAP tag (New England Biolabs #N9183S) was inserted after the Ptet or Plac promoter with the appropriate primers (supplementary methods) ...
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bioRxiv - Cell Biology 2023Quote: ... and SNAP-tag (New England BioLabs, N9181S) were used for tagging ...
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bioRxiv - Molecular Biology 2020Quote: Cleavage of the MBP tag from NlGr7 was completed according to manual instructions of the pMAL protein fusion and purification system (NEB, Inc, USA). The tag (MBP ...
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bioRxiv - Molecular Biology 2022Quote: ... The commercially available vaccinia virus capping system (NEB #M2080S) was used as a positive control.
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bioRxiv - Cell Biology 2020Quote: ... S141A ABHD11 and H296A ABHD11 with C-terminal eGFP tags or HA tags were created using NEBuilder HiFi (NEB). ABHD11 was also cloned into a transfection vector ...
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bioRxiv - Cancer Biology 2022Quote: ... AAK1(S-676D/E/A) and UVRAG (T-518D/E/A) using Q5-site directed mutagenesis Kit (NEB). Manufacturer’s instructions for mutagenic primer design were followed ...
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bioRxiv - Plant Biology 2023Quote: ... Strep–PIF4 recombined proteins were expressed in the Escherichia coli BL21 (DE3) strain and then purified using amylose resin (NEB, E8021S). DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Plant Biology 2020Quote: ... coli ER2566 cells (New England Biolabs). Expression of CBC-Qprot as a 15N-labeled protein and purification via Co-NTA affinity chromatography and electroelution was performed as described previously for the photosynthesis QconCAT protein (PS-Qprot ...
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bioRxiv - Cancer Biology 2021Quote: ... coli poly(A) polymerase (NEB Biolabs), and capped and 2’-O-methylated with Vaccinia Capping System (NEB Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells (New England Biolabs Inc.) and selected using antibiotics (Ampicillin).
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bioRxiv - Microbiology 2021Quote: ... coli DNA polymerase (New England Biolabs), and 0.25µL of RNaseH (New England Biolabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... coli DNA ligase (NEB, #M0205 L), 5 μl of E ...
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bioRxiv - Developmental Biology 2020Quote: ... coli DNA Polymerase (NEB, #M0209 L), 1 μl of dNTP (0 .2 mM) ...