Labshake search
Citations for New England Biolabs :
851 - 900 of 2156 citations for SARS CoV 2 Nucleoprotein His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... and 0.5 units/µl T4 RNA Ligase 2 (NEB, M0239S), with the enzyme added after the other components had been mixed and incubated at 23°C for 5 min ...
-
bioRxiv - Genomics 2020Quote: ... 2 μL of NEB DNA Quick Ligase (New England Biolabs) and 3 μL of Illumina Indexed adapter were added to the beads and incubated for 15 minutes at room temperature ...
-
bioRxiv - Genomics 2019Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Genomics 2019Quote: ... 2 μl of proteinase k (800 units ml-1, NEB) were added and reacted for 1 hour at 50°C ...
-
bioRxiv - Genomics 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2% DMSO and 1 U Phusion high fidelty polymerase (NEB) in 1x buffer provided by the manufacturer ...
-
bioRxiv - Genetics 2019Quote: ... 2 μl of 20 mg/ml proteinase K (NEB, P8107S) was added ...
-
bioRxiv - Microbiology 2019Quote: ... All cloning was performed with Q5 2× master mix (NEB) unless otherwise noted ...
-
bioRxiv - Neuroscience 2019Quote: ... alongside a 2-log DNA ladder (New England BioLabs Inc.). All primers sequence can be in Supplementary Table 1.
-
bioRxiv - Genomics 2020Quote: ... 2× LongAmp Taq Master Mix (25 μl; New England Biolabs) was used to synthesize second-strand cDNA using 2 μl of the PR2 primer (ONT) ...
-
bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Genomics 2021Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added to each well and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
-
bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated K227Q (200U; NEB)] was added ...
-
bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
-
bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
-
bioRxiv - Neuroscience 2020Quote: ... 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... 2 U of HindIII (New England Biolabs, Beverly, MA, USA), 2 U of EcoRI (New England Biolabs ...
-
bioRxiv - Systems Biology 2021Quote: ... PCR amplicons were purified from a 2% agarose gel (NEB), digested with EcoRI-HF and NotI-HF (NEB) ...
-
bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
-
bioRxiv - Cell Biology 2021Quote: ... diluted 2-fold in 1x G3 buffer (New England Biolabs) and incubated with 160 U endoglycosidase H (New England Biolabs ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 2 μL T4 DNA Polymerase (#M0203S, New England Biolabs). The reaction was incubated at room temperature for 45 min with gentle mixing every 5 min ...
-
bioRxiv - Microbiology 2021Quote: ... 2 µl of 20 µM SpCas9 (NEB, Cat. No. M0646M) was placed in the bottom of a 0.25 ml PCR tube ...
-
bioRxiv - Neuroscience 2020Quote: ... 12.5 μL NEBNext High-Fidelity 2× PCR Master Mix (NEB) were added and samples were PCR-amplified (72 °C 5 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
-
bioRxiv - Cell Biology 2021Quote: ... Then 2-5 unites of RNase H (New England Biolabs) in 5 µl of 1x RNase H buffer were added to the annealed RNA-chimeric oligo mixture and the RNase H cleavage reaction was performed at 37o C for 1 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... α2-3,6,8 neuraminidase (New England Biolabs, 25 U/μg protein) and α-N-acetyl-galactosaminidase (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (New England Biolabs), 1 µl of T4 or T7 DNA Ligase (New England Biolabs) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of T4 DNA Ligase Buffer (New England Biolabs), 1 µl of T4 or T7 DNA Ligase (New England Biolabs ...
-
bioRxiv - Biophysics 2022Quote: ... 2 μL (1000 U) PNGase F (New England Biolabs Inc) were added and the reaction was incubated for 6 h at 37 °C ...
-
bioRxiv - Biophysics 2022Quote: ... and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB) in extracellular buffer for 30 minutes at 37 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 100 units of 2′-O-Me-transferase (New England Biolabs) and 25 units RNasin Plus RNase inhibitor (Promega) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 μl of 2.5 mM dNTP (New England Biolabs N0447S), 0.75 μl of 50 mM MgCl2 ...
-
bioRxiv - Immunology 2022Quote: ... 2 mM ribonucleoside vanadyl complexes (New England Biolabs, Ipswich, MA), 0.02% ribonuclease-free bovine serum albumin (Thermo Fisher Scientific) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 10x T4 DNA ligase buffer (2 ul; Cat # B0202S; NEB), Bsa I (1ul ...
-
bioRxiv - Microbiology 2022Quote: ... 2 μl 10× T4 RNA ligase reaction buffer (NEB, M0242), 10 μl 50% PEG 8000 (NEB ...
-
bioRxiv - Biophysics 2019Quote: ... with 2 μl of 20 mM BG-GLA-NHS (NEB) in 20 μl 100 mM sodium borate buffer ...
-
bioRxiv - Genomics 2019Quote: ... Nuclei were resuspended in 1x NEBuffer 2 (New England Biolabs) with 1% SDS ...
-
bioRxiv - Immunology 2019Quote: ... 2 μl of T4 bacteriophage β-glucosyltransferase (T4-BGT, NEB) and ultrapure water to a final volume of 30 μl ...
-
bioRxiv - Genomics 2019Quote: ... and 1 µl of NEBuffer 2 (New England BioLabs #B7002S), then heating in a thermocycler at 95°C for 5 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl), 1.5 μl T4 DNA Polymerase (NEB ...
-
bioRxiv - Genomics 2019Quote: ... 2 µl of NEBNext FFPE DNA Repair Mix (M6630, NEB), 3.5 µl of NEBNext Ultra II End Prep Reaction Buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 μl T4 PNK (NEB, cat#M0201S, 10 U/μl) and 1.5 μl Klenow Fragment (3’->5’ exo- ...
-
bioRxiv - Plant Biology 2020Quote: ... 2 µl of Taq 5× Master Mix (New England Biolabs) and Milli-Q water ...
-
bioRxiv - Systems Biology 2021Quote: ... using the NEBuilder HiFi DNA Assembly Master Mix (2×) (NEB) for 20–40 min at 50 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.2μl of 2 units of Taq1 enzyme (New England Biolabs) and 3.8μl of dH20 ...
-
bioRxiv - Biochemistry 2021Quote: ... Five microlitres of 2× RNA loading dye (New England Biolabs) was added ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 μg of genomic DNA was digested with TfiI (NEB) and StyI (NEB) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 2 μl of DpnI restriction enzyme (New England Biolabs) were added and mixed by gentle flicking to prevent shearing of gDNA ...
-
bioRxiv - Microbiology 2021Quote: ... All cloning was performed with Q5 2 × master mix (NEB) unless otherwise noted ...