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Citations for New England Biolabs :
851 - 900 of 1175 citations for Recombinant Mouse ADIPOQ Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Microbiology 2024Quote: ... Samples were resolved on SDS-PAGE gels alongside a Blue Protein Standard Broad Range ladder (New England BioLabs). Bands were transferred onto nitrocellulose membranes and were blocked with a 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... one of the immunoprecipitated samples was treated with 400 units of Lambda protein phosphatase (New England Biolabs, P0753S) at 30 °C for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... the Gbait and dld gRNAs were combined at 4.8uM each with 20uM Lba Cas12a protein (New England Biolabs) and incubated for 10 minutes at 370C ...
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
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bioRxiv - Biochemistry 2023Quote: ... and then deglycosylated and dephosphorylated overnight at 37 °C with the protein deglycosylation mix II (New England Biolabs) and Quick CIP (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Selected candidates were expressed in vitro using the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were expressed and purified from 2 to 4 liters of Escherichia coli NiCo21(DE3) (New England Biolabs) as previously described (24) ...
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bioRxiv - Genetics 2023Quote: Total protein extracts were prepared with 2.106 cells re-suspended in 50 μL of loading buffer (B7709S, Biolabs) containing 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were purified using the amylose resin affinity purification method by following the manufacturer’s recommendations (E8021L, NEB). Briefly ...
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bioRxiv - Neuroscience 2024Quote: HA-tagged BirA-Tau fusion proteins were generated by using the NEBuilder™ HiFi DNA Assembly Kit (NEB). To this end ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Neuroscience 2022Quote: ... CREB (CCDS15005.1) and CRTC1 (CCDS40372.1) were amplified from mouse brain cDNA library by primers contain AgeI-HF (R3552L, NEB) and BamHI-HF (R3136L ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... mCapH2 (Gene ID: 52683) genes were PCR amplified from human and mouse genomic DNA with Q5 polymerase (NEB). The lengths of homology arms are featured in electronic supplementary material Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Neuroscience 2024Quote: Mouse Hapln1 (NM_013500) was cloned into the pAAV vector by PCR with the following primers and ligase (NEB) or In-Fusion cloning (Takara) ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were stopped by adding EDTA and the proteins were digested by adding Proteinase K (New England Biolabs) at 37℃ for 20min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 5U Enterokinase (cat#: P8070S, NEB) in the 1x EK reaction buffer (20 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1.5 mg of protein was cleaved in a 2 ml reaction with 240 Units of TEV protease (NEB) for two hours at 30 °C ...
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bioRxiv - Physiology 2021Quote: ... The biotinylated peptides were placed on streptavidin-coated plates and three rounds of solid-phase panning were conducted using 109 random 12-mer peptides fused to a minor coat protein of M13 (pIII) following the manufacture’s protocol (Ph.D-12 Phage Display Peptide Library Kit, NEB, E8110S). Sequenced data were subsequently analyzed as follows ...
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bioRxiv - Bioengineering 2021Quote: ... and cleavage of the fusion protein with enterokinase that were performed according to manufacturer’s instructions (amylose resin purification – according to pMAL Protein Fusion & Purification System, New England Biolabs, Ltd. ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg of “heavy” or “light” THP-1 protein extract were dephosphorylated with 50 U of Antarctic phosphatase (NEB) in Antarctic phosphatase buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Cell Biology 2021Quote: ... beads were pelleted and resuspended in 100 μl of phosphatase buffer with lambda protein phosphatase (P0753; New England Biolabs) before agitation at 30°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 58,000 × g for 50 minutes at 4°C and the protein was batch purified using chitin beads (NEB). Protein-bound chitin beads were washed with lysis buffer and high salt buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: All recombinant CPK28 clones were transformed into lambda phosphatase-expressing BL21 (DE3) E.coli cells for the production of dephosphorylated proteins or into T7 Express cells (New England Biolabs) for production of hyperphosphorylated protein (53) ...
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bioRxiv - Microbiology 2021Quote: ... benthamiana using the method described in the immunoblotting section were treated with Protein Deglycosylation Mix II (New England BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... As a marker 70 µl of Color Protein Standard Broad Range (New England BioLabs, #P7712S; 25 to 245 kD) diluted 1/7 in SDS sample buffer was loaded ...
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bioRxiv - Cell Biology 2021Quote: ... One sample was left untreated and the other two were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs) ...
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bioRxiv - Biophysics 2021Quote: ... All proteins were purified and labeled with BG-surface Alexa 647 and BC-surface Dy547 dyes (New England Biolabs) as previously described (Murayama and Uhlmann ...
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bioRxiv - Cell Biology 2020Quote: The remainder of the protein was incubated with 50 μl of Ni-NTA Magnetic Beads (New England Biolabs, Inc.) on a rocker for 45 min at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1:8 dilution Cas9 protein (New England Biolabs, cat. M0386M; diluted in buffer B, New England Biolabs, cat. B802S) and 1:40 dilution of phenol red (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were boiled in 1X Denaturing Buffer and incubated with PNGase F or Endo Hf (New England Biolabs) for 1-1.5 hr at 37°C following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Biochemistry 2019Quote: ... All sens RNA strands were labeled at their 5’ end with [γ-32P] ATP using protein nucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... 115 amino acids) fused to the C-terminus of maltose binding protein (MBP) in pMALX vector (New England Biolabs), MBP-5-HT3A-ICD-pMALX ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of genomic DNA was incubated with 3 µg of in vitro transcribed sgRNAs and 3 µg of purified Cas9 protein in 20µl of 1x NEB3 buffer (New England Biolabs) at 37°C over night ...
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bioRxiv - Developmental Biology 2019Quote: ... single copy scGESTALT.2 F1 male transgenic adults were crossed to wildtype female adults and one-cell embryos were injected with 1.5 nl of Cas9 protein (NEB) and sgRNAs 1-8 in salt solution (8 µM Cas9 ...