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Citations for New England Biolabs :
801 - 850 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were treated with 5′ RNA polyphosphatase (Epicenter RP8092H) and ligated to 3′ pre-adenylated adapters with Truncated T4 RNA ligase (NEB M0373L). Small RNAs were then hybridized to the reverse transcription primer ...
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bioRxiv - Biochemistry 2023Quote: ... oligonucleotide X12-3 HJ3 (93 nt) was labeled at the 5’ terminus with [γ-32P] (Hartmann-Analytic) and T4 polynucleotide kinase (New England Biolabs), according to standard protocols65 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the annealed substrates was 5′-end-labelled with [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs). For fluorescently labeled HJ40 substrates ...
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bioRxiv - Microbiology 2023Quote: ... The 5’ termini of all ten DNA fragments (F1-F9 and the linker) were phosphorylated by using T4 PNK (NEB; #M0201), and the equimolar amounts (0.05 pmol each ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting full-length cDNA was gel-purified and ligated to the 5′ adaptor OWG920 with High Concentration T4 RNA Ligase (NEB M0437M) overnight on a shaking thermomixer ...
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bioRxiv - Immunology 2023Quote: ... The digested and purified inset and vector were ligated at a ratio of 5:1 using T4 DNA ligase (NEB, M0202) overnight at 18°C ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated to pre-adenylated linkers (NI-810 to NI-815) containing 5 nt sample barcodes unique for each sample using truncated T4 RNA ligase 2 (K227Q) (NEB; M0351L). Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Precipitated RNAs were resuspended in Milli-Q water and labeled at the 5’-end with [γ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs). Excess [γ-32P]-ATP was removed by G-25 MicroSpin column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: The library was prepared following our previously reported procedure with slight changes.[21] 3′-End repair and 5′-phosphorylation were conducted with T4 polynucleotide kinase (PNK) (NEB, M0201S). 16 µL RNA was mixed with 2 µL 10× T4 PNK reaction buffer and 1 µL T4 PNK ...
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enyzme were purchased from New England BioLabs (NEB, Germany). PCR was performed using Q5 High Fidelity 2X Master Mix (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... and subsequently end repair (T4 DNA polymerase, Klenow DNA polymerase and T4 Polynucleotide Kinase; New England Biolabs, USA) and A-tailing (Klenow Fragment ...
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bioRxiv - Cell Biology 2020Quote: ... Paired barcode and splints were phosphorylated in a PCR plate by combining 20 μl of oligonucleotides with 40 μl phosphorylation mix (1X T4 ligation buffer, 0.2 mg/ml BSA, 0.167 U/μl T4 PNK (NEB)) and incubated for 30 min at 37°C and heat inactivated for 20 min at 65°C ...
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bioRxiv - Molecular Biology 2020Quote: ... gRNA oligos were annealed using T4 Polynucleotide Kinase (Fisher# 50811602) in T4 ligation buffer (New England Biolabs# B0202S) and then inserted into pU6-2-gRNA plasmids using T4 DNA ligase (New England Biolabs # M0202S).
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bioRxiv - Biophysics 2023Quote: ... we ligated the two fragments together using a T4 DNA ligase in T4 ligase buffer (New England Biolabs), containing 1 mM ATP overnight at 16°C ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The fragments were ligated together by using a T4 DNA ligase in T4 ligase buffer (New England Biolabs), with 1 mM ATP overnight at 16°C ...
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bioRxiv - Molecular Biology 2023Quote: ... mixed with 3 µl T4 DNA ligase and 3 µl T4 DNA ligase buffer (New England Biolabs, M0202S), and incubated at 25°C overnight (16 hr) ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was resuspended in buffer IV (160 µl annealed oligos, 1.2x T4 ligase buffer, 6,000 U T4 ligase, 1x protease inhibitors; NEB) to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was resuspended in buffer IV (160 µl annealed oligos, 1.2x T4 ligase buffer, 6,000 U T4 ligase, 1x protease inhibitors; NEB) to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then 3’dephosphorylated by denaturing at 65°C for 5 min and incubating with T4 PNK (NEB, catalog no. M0201S) in a 10 μL reaction (7 μL precipitated RNA ...
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bioRxiv - Microbiology 2021Quote: ... and total of 5 μg of the five fragments was ligated in an equal molar ratio by T4 DNA ligase (New England Biolabs, Ipswich, MA) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... containing a 5′-Biotin-PC group and a 3’-OH (Sup. Table. 2) were ligated to the 5′ end of RNAs using T4 RNA ligase (NEB, Cat #M0204S) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: In vitro transcribed RNAs were treated with Antarctic phosphatase (Fermentas, Waltham, MA) to remove the 5’ terminal phosphate and then labeled by T4 polynucleotide kinase (New England Biolabs, Ipswitch, MA) in the presence of γ-32P-labeled ATP (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’ adapters with 4 terminal randomized nucleotides at the 3’ end was added using T4 RNA ligase (New England Biolabs, cat# M0204S). Ligation was carried out for 1 hour at 25°C with 20% PEG8000 ...
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bioRxiv - Plant Biology 2020Quote: ... and T4 DNA ligase (New England Biolabs), generating two sgRNA modules pMR217-sgRNA-LecRK-I.1 and pMR218-sgRNA-LecRK-I.6 ...
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bioRxiv - Synthetic Biology 2021Quote: ... phosphorylated by T4 PNK (New England Biolabs), blunt-end ligated using T4 ligase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.5μL of T4 DNA ligase (NEB, #M0202S), 2 μL 10x T4 DNA ligase buffer and 12.5 μL of nuclease free water ...
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bioRxiv - Molecular Biology 2020Quote: ... using T4 polynucleotide kinase (New England Biolabs) and separated on a 12% denaturing polyacrylamide gel ...
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bioRxiv - Cell Biology 2020Quote: ... and 400U of T4 DNA ligase (NEB) in a 20 μL reaction volume ...
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bioRxiv - Immunology 2021Quote: ... oligos were phosphorylated with T4 PNK (NEB) for 30 minutes at 37°C ...
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bioRxiv - Genetics 2021Quote: ... with Esp3I and T4 DNA ligase (NEB). A combination of colony PCR ...
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bioRxiv - Genomics 2020Quote: ... and 2 μL T4 PNK (NEB, M0201L) were added to the sample in respective order ...
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bioRxiv - Microbiology 2020Quote: ... in 1X T4 RNA Ligase Buffer (NEB), 9% DMSO ...
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bioRxiv - Microbiology 2021Quote: ... and ligated using T4 DNA ligase (NEB) to produce pMV261::hsp60-cds1 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1x T4 DNA Ligase buffer (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... and ligated with T4 DNA ligase (NEB). 2 μl of the ligation product was transformed into bacteria using XL10-Gold Ultracompetent Cells (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... in 10X T4 DNA Ligase buffer (NEB). Ligation reaction were incubated for at least 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 U of T4 DNA polymerase (NEB), 9 U of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... 9 U of T4 Polynucleotide Kinase (NEB) and 1 U of Klenow fragment (NEB ...
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bioRxiv - Genomics 2020Quote: ... in 1x T4 DNA ligase buffer (NEB) with 4 μl (400 U/μl ...
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bioRxiv - Synthetic Biology 2022Quote: ... or ligation using T4 DNA ligase (NEB). Plasmids were transformed into either competent NEB 5-alpha F’Iq (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... 1x T4 DNA ligase buffer (B0202, NEB) and T4 PNK (M020 ...
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bioRxiv - Molecular Biology 2020Quote: Using T4 RNA ligase 1 (NEB, M0437M), total RNA was isolated from 10-week-old or senescent rosettes of WT plants were ligated to a specific oligo adaptor at their 5’terminal ...
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bioRxiv - Molecular Biology 2020Quote: ... 7.5U T4 DNA polymerase I (NEB, M0203), 2.5U DNA polymerase I ...
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bioRxiv - Genomics 2022Quote: ... 10 μl of T4 DNA ligase (NEB), and 3 μl of nuclease-free water ...
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bioRxiv - Genomics 2020Quote: ... using 20U T4 polynucleotide kinase (NEB, M0201S). Subsequently RNA probes were purified by 5% Urea PAGE extraction and RNA clean & concentrator-5 Kit ...
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bioRxiv - Genomics 2019Quote: ... and ligated with T4 DNA ligase (NEB) overnight at 16°C ...
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bioRxiv - Genomics 2020Quote: ... using T4 DNA ligase (New England BioLabs) for 20min at room temperature ...