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Citations for New England Biolabs :
701 - 750 of 930 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was discarded and the nuclei were resuspended in 300 μl Fill-in Buffer containing 30 μl Klenow Buffer 10X (NEB, M0210L), 15 μl1 mM Biotin-16-dCTP (Jena Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclei pellet was resuspended in the Ligation Buffer containing 120 μl 10X NEB T4 DNA Ligase Buffer supplemented with 10 mM ATP (NEB, B0202), 60 μl 20% Triton X-100 (1% final) ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated with 100 μl CutSmart buffer containing 5 mM DTT and 1 mM dATP for 1 hour at room temperature before incubation for 2 h at 37°C in another 100 μl of the same buffer containing 1 μl Klenow exo-(NEB M0212S) and 1 μl T4 PNK (NEB M0201S) ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Physiology 2019Quote: ... The obtained 20-nt sequences were incorporated into a DNA oligonucleotide template containing a T7 promoter and a sgRNA scaffold by overlapping PCR using Phusion high fidelity DNA polymerase (New England Biolabs, M0530). The primer sequences for overlapping PCR were as previously described11 including the designed sgRNA sequences ...
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bioRxiv - Microbiology 2019Quote: ... and 1 ml of lysate (corresponding to 50 ml of the original culture) was transferred into tubes containing 50 μl of pre-washed (3x) magnetic α-HA beads (NEB) and incubated for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: PCR amplification was performed using 2 μl of the diluted cDNA in a 25 μl reaction mix containing 0.02 U of Q5® High-Fidelity DNA Polymerase (NEB, Germany), 200 μM of dNTP ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were diluted to 0.1 OD595 in 1 mL of EMM5S containing 1 µM of silicon-rhodamine benzylguanine derivative SNAP-SiR647 (SNAP-Cell® 647-SiR, New England Biolabs). Culture tubes were wrapped in aluminum foil to protect them from light and incubated on a rotator overnight (about 15 hours ...
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bioRxiv - Genomics 2021Quote: ... Samples were then combined and AMII adapters containing the motor proteins needed for sequencing were ligated using NEBNext® Quick Ligation Module (NEB). AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: PCR of the tagged DNA was performed in 25 μl reactions in 200 μl thin-walled PCR tubes containing 1X Q5® Buffer (NEB), 1X Q5® GC Enhancer (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... The amplification of bacterial DNA was achieved by targeting the V3–V4 region of 16S rRNA gene with 30 µL final volume containing 15 µL of 2× master mix (BioLabs, USA), 3 µL of template DNA ...
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bioRxiv - Cell Biology 2021Quote: The recovery probe was prepared as follows: A standard 100 µl PCR reaction was prepared containing 10 ng of bacteriophage λ DNA (NEB), 5 units of Taq polymerase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... The gRNA template was generated using a forward and reverse oligonucleotide (IDT, HPLC purity) containing BbsI overhangs that were annealed and phosphorylated using T4 Polynucleotide kinase (PNK) (New England Biolabs, M0201S). Next ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 575 embryos were injected with a mixture containing 300 ng/μL of sgRNA (one guide) and 600 ng/μL of Cas9 protein (NEB, M0641) while for Dll ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated in 2i medium containing 300 nM SNAP-Cell 647-SiR (New England Biolabs, Ipswich, MA, USA, S9102S) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: The positive CTR plasmid was created by PCR amplification of the complete vector containing the mGFP-FGFR3 expression construct using the high-fidelity polymerase Q5 (NEB, USA). Specific primers were designed to delete the stop codon of FGFR3 and to also add the recognition sequence for the AgeI restriction enzyme ...
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bioRxiv - Microbiology 2020Quote: ... The flies were homogenised in 100 μl of TE-buffer pH 8 containing 1% Triton X-100 and 1% Proteinase K (NEB, P8107S). Homogenates were incubated for 3 h at 55°C followed by a 10 min incubation step at 95°C ...
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bioRxiv - Molecular Biology 2020Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 2 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml tris buffer (10 mM Tris HCl pH 8.0) ...
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bioRxiv - Cancer Biology 2020Quote: ... the oligonucleotides containing the different gRNA-pairs (Supplementary Table 4) were amplified with Phusion High-Fidelity polymerase (New England Biolabs, M0530S) using primer F5 and R1 (Supplementary Table 2) ...
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bioRxiv - Genomics 2022Quote: ... approximately 500 ng of gDNA and hACE2 payload DNA containing mActb gene on the backbone were digested with EcoRI (NEB, R3101S) at 37°C for 2 hours ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmid pGEMM7 was assembled from seven PCR fragments containing independent transcriptional cassettes and the plasmid backbone of pUC19 (New England Biolabs, USA) (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg protein were denatured as above and adjusted to a final concentration of 1x Glycobuffer 2 containing 125U PNGase F per μg (NEB, P0704S) in 50 μl ...
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bioRxiv - Microbiology 2020Quote: ... Colony PCRs were carried out in 12.0 μL final volumes containing: 5 μL of high-fidelity OneTaq® QuickLoad® 2X Master Mix (New England Biolabs), appropriate forward and reverse control primers (both 0.4 μM ...
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bioRxiv - Biochemistry 2020Quote: ... chromatin and chromatin bound fractions were released from magnetic beads using binding buffer containing 5 mM CaCl2 plus an excess (2000 units/ 20 mL reaction) of micrococcal nuclease (MNase; NEB, M0247S) Beads were incubated for 5 minutes at 37 °C with shaking (1250 rpm) ...
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bioRxiv - Genetics 2020Quote: ... In vitro transcription template was amplified with T7 promoter sequence containing primer and in vitro transcribed using T7 RNA polymerase (NEB, E2040S). The IVT RNAs were capped using Vaccinia virus capping enzyme (Cellscript ...
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bioRxiv - Genomics 2021Quote: ... and cloned into Drosophila STARR-seq vectors containing either the DSCP or Rps12 core-promoters using Gibson cloning (New England BioLabs; E2611S). The oligo library for human STARR-seq screens was amplified (primers see Supplementary Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... The 5’ RNA linker (5’-blocked and containing a 10 nt UMI (/5AmMC6/GUUCAGAGUUCUACAGUCCGACGAUCNNNNNNNNNN3’) was ligated to the RNA fragments on bead using T4 RNA ligase (NEB M0437) for 2 h at 24°C with shaking ...
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bioRxiv - Synthetic Biology 2021Quote: 8.3 μM bdSUMO-HSPB611–20 fusion protein containing pSer or nhpSer at site S16 of HSPB6 were reacted with 3 units of λ phosphatase (NEB) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... Toe-printing reactions were carried out in 5-µl aliquots containing a PURExpress transcription-translation coupled system (New England Biolabs, USA) to which the test template was added (16) ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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Enhancers display constrained sequence flexibility and context-specific modulation of motif functionbioRxiv - Genomics 2022Quote: ... Drosophila library fragments were amplified (primers see Supplementary Table S1) and cloned into Drosophila STARR-seq vectors containing the DSCP core-promoters using Gibson cloning (New England BioLabs; E2611S). The oligo library for human STARR-seq screens was amplified (primers see Supplementary Table S1 ...
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bioRxiv - Genomics 2022Quote: ... 10 U of the enzyme was used in the 50 μl-reaction containing 5 μl of rCutSmart Buffer (10X, NEB # B6004S) for 30 min-incubation at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and myc genes into a retroviral vector (containing CD19 CAR gene) using NEB HiFi DNA Assembly Master Mix (NEB cat. # E2621S).
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bioRxiv - Immunology 2022Quote: Point mutations were introduced through linearizing the plasmid by PCR with primers containing the intended mutations (Supp. Table S1) followed by reverting to circular plasmid with Gibson Assembly Master Mix (M5510A, NEB, USA).
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... the total 60 μL of end-prepped DNA from the previous step were added in a mix containing 25 μL of Ligation Buffer LNB (NEBNext Quick Ligation Module, New England Biolabs, UK), 10 μL NEBNext Quick T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified fragment containing SNAP was assembled with an amplified pUbq destination backbone using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pUbq-SNAP destination vector ...
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bioRxiv - Molecular Biology 2023Quote: Internally fluorescein-labelled RNA was produced by ligation of in vitro transcribed 5′ acceptor RNA fragment with chemically produced 3′ donor RNA containing internal fluorescein dT modification and 5′ monophosphate essential for ligase activity using T4 Ligase 2 (NEB #M0239S). Additionally ...
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bioRxiv - Molecular Biology 2023Quote: ... The tube containing the plugs was then cooled to 42°C before adding 5 µl of Beta-Agarase I (New England Biolabs, M0392) and incubating at 42°C for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
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bioRxiv - Genomics 2024Quote: ... The genomic DNA was incubated in a mix containing S-Adenosyl methionine (SAM) along with the DNA adenine Methyltransferase (dam, NEB, USA) and its corresponding buffer for 4 hours at 37°C ...
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bioRxiv - Genomics 2023Quote: Nuclei were resuspended in 200 uL of Methylation Reaction Buffer (Buffer M containing 1mM S-adenosylmethionine (SAM)) (New England BioLabs B9003S). 10uL high-concentration EcoGII was added per 1e6 nuclei and the nuclei suspension was incubated at 37°C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: We first generated a transfer vector containing a reporter gene (hsp68 promoter + mScarlet) flanked by φC31 and BxbI attB sites using HiFi Assembly (NEB #E2621). The insulator sequences were obtained from their respective papers (cHS4 from Chung et al.9 ...
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bioRxiv - Molecular Biology 2022Quote: PANK3 mutations were made in a gateway compatible pDONR plasmid containing a PANK3 ORF (a gift from the lab of Ben Cravatt) by amplifying the whole plasmid with primers containing the desired mutations and using HiFi DNA Assembly Master Mix (NEB, # E2621) to re-circularize the amplicon ...
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bioRxiv - Microbiology 2022Quote: ... the CPER product was subject to post-PCR nick sealing for 30 min at 50°C and 30 min at 60°C in a 25 μL reaction containing 1 mM β-nicotinamide adenine dinucleotide (NAD+) (NEB) and 0.5 μL HiFi Taq DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... pCS2+ backbones containing ACVR1 or an FOP-ACVR1 mutant were PCR amplified with the mutant primer using Phusion high fidelity polymerase (NEB, M0530). Mutated plasmids were transformed into Top10 chemically competent cells and DNA from individual colonies was submitted for Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-5 μM SNAPf fused activator was incubated in a 1× PBS solution containing up to threefold excess of SNAP-surface 649 (New England BioLabs, #S9159S), 1 mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... Type-IIS cloning was performed as described previously (Cai et al., 2020) using a Master Mix containing 10% (v/v) 10× T4 DNA ligase buffer (NEB #M0202), 2.5% (v/v ...
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bioRxiv - Genetics 2023Quote: ... the PCR products containing the geneticin1-664-PgpdA-RB and the SpecR-Ori were digested with SapI (New England Biolabs, UK) and ligated with T4 DNA ligase ...