Labshake search
Citations for New England Biolabs :
651 - 700 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Q5 High-Fidelity DNA Polymerase (M0491S, NEB) was used with 200 ng of template DNA in a total reaction volume of 20 µL.
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified T7 PCR products were confirmed by sequencing and transcribed into dsRNA using the T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). The dsRNA produced was purified via ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNAs were synthesised by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... In vitro transcription was done using the HiScribe T7 High Yield RNA Synthesis Kit according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... dsDNA templates were then in vitro transcribed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA) using overnight incubation as described in the protocol for <0.3kb transcripts.(28 ...
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bioRxiv - Genomics 2021Quote: ... 1.5 µg of high-molecular-weight genomic DNA was subjected to end repair using the NEBNext Ultra II End Repair kit (NEB, cat. #E7445) and purified using 1x AmPure beads (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Around 1 µg of each PCR product was used as template for dsRNA synthesis using the HiScribe T7 High Yield RNA synthesis kit (New England BioLabs, Cat # E2040S) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Amplification of cDNA was conducted using T7 forward primers and cDNA-based RNA was generated using HiScribeTM T7 Quick High-Yield RNA Synthesis kit (New England Biolabs, Ipswich, MA). T7 RNA (1 μg ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries for sequencing were made from high quality RNA that were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA templates were generated by PCR (26) and used for synthesis of sgRNAs using NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: The 16S rRNA V4 region was amplified using the following primers 515f-Y: GTGYCAGCMGCCGCGGTA and 806r-N: GGACTACNVGGGTWTCTAAT and The Q5® high-fidelity DNA polymerase kit (New England BioLabs, UK). 2μl of extracted DNA was added to a PCR reaction mix prepared by mixing a final concentration of 1X Q5 reaction buffer ...
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bioRxiv - Microbiology 2021Quote: The synthesized oligo pool of CRISPRa library was PCR amplified with primers (table S5) including the BsmBI recognition sites using Phusion®High-Fidelity PCR Kit (NEB, E0553L). After purification with DNA Clean & Concentrator-25 (Zymo Research Corporation ...
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bioRxiv - Microbiology 2020Quote: ... and one fragment of about 320 bp of the 3’-terminal region by 3’ RACE were amplified using Phusion High-Fidelity PCR Kit (New England Biolabs, MA, USA) under the following conditions [98°C ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting single stranded cDNA was further In-vitro transcribed (IVT) using HiScribe T7 Quick High Yield RNA Synthesis Kit according to the manufacturer’s protocol (New England BioLabs Inc, NEB #E2050). The amplified RNA was than purified using Monarch RNA Cleanup Kit (New England BioLabs Inc. ...
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bioRxiv - Genomics 2022Quote: ... wild-type and mutant NEAT1 RNA fragments were transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and labelled with Biotin using Biotin RNA Labelling Mix (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... IVT reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit using 300 ng of PCR product as template (NEB Cat. #E2040S). After an incubation for 16 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA synthesis was performed by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs, Ipswich, MA, USA). Purified sgRNA (0.5 μg ...
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bioRxiv - Genomics 2021Quote: ... 1.5 µg of high-molecular-weight genomic DNA was subjected to end repair using the NEBNext Ultra II End Repair kit (NEB, cat. #E7445) and purified using 1x AmPure beads (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The T7 PCR template was used to prepare dsRNA with the HiScribe T7 Quick High Yield RNA Synthesis Kit according to the manufacturer protocols (New England BioLabs, MA, USA). For the injection experiments ...
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bioRxiv - Cancer Biology 2022Quote: ENO1-3’UTR and G6PD-CDS IDT PAGE purified oligos (Supplementary Table S6) containing T7 promoter were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB E2040). RNA was purified using TRIzol (ThermoFisher,15596026 ...
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bioRxiv - Microbiology 2022Quote: RNA was in vitro transcribed from 1 μg of agarose gel-purified band corresponding to the intended size using HiScribe™ T7 high yield RNA synthesis kit (cat#E2040S, New England Biolabs Inc) followed by adding a 5’ cap using the Vaccina Capping System (cat#M2080S ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Genomics 2024Quote: Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described in48 ...
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bioRxiv - Biophysics 2023Quote: Purified linear and circular DNA were both in vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S). 240 ng reaction was prepared according to the manufacturer’s recommendation (see details in Supplementary Methods) ...
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bioRxiv - Microbiology 2023Quote: ... AvcI RNA was synthesized by in vitro transcription using the T7-AvcI reverse complement DNA template and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB™). Bio-11-UTP was included during the transcription reaction for Northern blot detection purposes ...
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bioRxiv - Systems Biology 2023Quote: ... Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described previously86 ...
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bioRxiv - Biochemistry 2023Quote: ... Wild-type or mutant plasmid was linearized using restriction digestion and then subsequently used for in vitro transcription (HiScribe® T7 High Yield Synthesis Kit, NEB E2040) to synthesize wild-type and mutant N gene RNA ...
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bioRxiv - Biophysics 2023Quote: ... 1 µg of the duplex was in vitro transcribed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB # E2050S) following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Molecular Biology 2023Quote: ... viral RNA was produced via in vitro transcription of an XbaI-linearized HCV-Jc1 or AflII-linearized YFV-17D plasmid using the HiScribe® T7 High Yield RNA Synthesis Kit (HCV, New England Biolabs E2040S) or mMESSAGE mMACHINE™ SP6 Transcription Kit (YFV-17D ...
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... sgRNA complementary to the central part of the hACE2 (Table 2) was synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA). Library was loaded onto the FLO-MIN106 flow cell and sequenced with GridION (Oxford Nanopore ...
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bioRxiv - Immunology 2024Quote: ... The T7 sgRNA PCR product (500 ng) was used as a template for in vitro transcription with the T7 High Yield RNA synthesis kit (NEB, cat # E2040S). The reaction was incubated at 37°C for 4 hours ...
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bioRxiv - Plant Biology 2021Quote: ... High fidelity PCR was conducted on the abcg43-1 line using the Q5 High-Fidelity 2X Master Mix (NEB). PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Genomics 2022Quote: The library for high-throughput sequencing was prepared in a two-step PCR (Q5 high-fidelity DNA polymerase, NEB). In PCR1 ...
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bioRxiv - Bioengineering 2022Quote: ... Templates for IVT were generated by performing high-fidelity PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) and primers 1 and 2 (IDT ...
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bioRxiv - Plant Biology 2021Quote: ... Phusion high-fidelity DNA polymerase (Fermentas) or with the Q5® High-Fidelity DNA Polymerase (New England Biolabs, USA) using an Eppendorf (Germany ...
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bioRxiv - Bioengineering 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase or Q5 High-Fidelity 2X Master mix (New England Biolabs). PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For molecular cloning or any other application requiring high sequence fidelity Q5 High Fidelity 2X Master Mix (NEB, M0492S) was used ...
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bioRxiv - Genomics 2020Quote: The library was prepared for high-throughput sequencing in two rounds of PCR (Q5 High-Fidelity DNA Polymerase, NEB). In PCR1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was digested using SphI High Fidelity and MluCI High Fidelity enzymes with the appropriate CutSmart Buffer (New England Biolabs) and cleaned with AMPURE XP beads ...
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bioRxiv - Neuroscience 2021Quote: ... were used for amplification with Platinum Taq DNA Polymerase High Fidelity or Q5 High Fidelity DNA Polymerase (New England BioLabs), followed by cloning as per the TOPO TA Cloning Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the N gene was amplified from the BAC containing viral cDNA with a high fidelity polymerase (Taq Q5 Hot Start High-Fidelity 2x Master Mix, Ref. M0494S, NEB) and primers PV012-F (5’-ACT-GTA-ATA-CGA-CTC-ACT-ATA-GGG-ATG-TCT-GAT-AAT-GGA-CCC-CAA-AAT-C-3’ ...
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bioRxiv - Systems Biology 2021Quote: ... PCR reactions with plasmid and genomic DNA templates were performed using the Phusion High-Fidelity 2× Master Mix or Q5 High-Fidelity 2× Master Mix (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... homologous recombination domains (HRDs) were generated using high fidelity Taq DNA polymerase (Q5 high-fidelity DNA polymerase, NEB, Ipswich, MA) and polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from a high-titer ϕHNL01 lysate and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs, Ipswich, MA, USA). Libraries were quantified using a BioAnalyzer (Agilent ...