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Citations for New England Biolabs :
601 - 650 of 1104 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Inserts were amplified by PCR from diluted ΦKZ lysates and joined into the linearized vector by Hi-Fi DNA Assembly (NEB). The resulting vectors were used to transform E ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... homology arms flanking the region to be deleted were obtained using polymerase chain reaction (PCR) and cloned into the pExG2-KanR suicide vector using Hi-Fi Assembly (New England Biolabs)70 ...
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bioRxiv - Microbiology 2023Quote: ... was used as a template for in vitro transcription using Hi-T7 RNA Polymerase and Ribonucleotide Solution Mix (both from New England Biolabs) in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were amplified for 12 PCR cycles with unique dual index primers using the NEBNext Hi-Fi 2X PCR Master Mix (New England Biolabs). Amplified libraries were purified using a 1.2X ratio of Axygen magnetic beads (Corning Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were separated from lysates and incubated with affinity beads at 4°C overnight (His beads: Ni-NTA from QIAGEN; GST beads: glutathione-agarose beads from ROTH; MBP beads: amylose beads from NEB). Protein-binding beads were collected by centrifugation and washed several times with washing buffer (His washing buffer ...
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bioRxiv - Genetics 2023Quote: ... The final cleaned-up Hi-C library was used as input material for Illumina sequencing library prep kit (NEB, E7805) with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... by PCR amplification with the indicated primers (Key Resources table) followed by a digestion with Bam HI and Xba I enzymes (New England Biolabs) for 37°C 2 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... linker was inserted into the pC13N-iCAG.copGFP vector through BsrGI and MluI digestion and Hi-T4™ ligation (Cat.#M2622; New England Biolabs), thus replacing copGFP and generating the pC13N_N-HiBiT plasmid ...
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bioRxiv - Microbiology 2023Quote: ... The insert was amplified using MRSN390231 overnight culture as a DNA template and joined with linearized pUC18-mini-Tn7T-LAC vector using Hi-Fi DNA Gibson Assembly (NEB) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Inserts were amplified by PCR using bacterial overnight culture or synthesized by Twist Bioscience and joined with the digested vector using Hi-Fi DNA Gibson Assembly (NEB) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... was used in sequential reverse transcription and PCR amplification steps using the Protoscript II first strand cDNA synthesis kit and Q5 Hi-fidelity DNA polymerase (NEB) according to manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: ... The TRAK1 construct was made through assembling His-ZZ-TEV-SNAP tag and TRAK1(1-395) into a pACEBac1 vector via HIFI DNA assembly kit (NEB). All constructs were verified using whole plasmid sequencing from Plasmidsaurus.
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bioRxiv - Cancer Biology 2022Quote: ... 5 µg DNA was digested with 5 µl RNase H (NEB, M0297), 3 µl Hind III (Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... triplex reaction was treated with either 5 units of RNase H (NEB) or 10εg of RNase A (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... The remaining glycopeptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc residue at the corresponding site ...
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bioRxiv - Genomics 2019Quote: ... For that the mix containing 2 µL of RNAse H (NEB, #M0297S), 1 µL of E ...
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Membrane integration and topology of RIFIN and STEVOR proteins of the Plasmodium falciparum parasitebioRxiv - Biochemistry 2019Quote: ... and 0.75 μl of Endo H (500,000 units/ml; NEB, MA, USA) or dH2O (mock sample) ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was degraded using 5 units RNase H (New England Biolabs M0297) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was then degraded by addition of RNase H (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... eluted in 27 μl of water and subjected to RNase-H (NEB) digestion at 37°C for 30 min followed by heat inactivation ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) or anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... The hybrid was reacted with 1 μl RNase H (New England BioLabs) at 37 °C for 30 min and then heated to 90 °C for 10 min to terminate the reaction ...
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bioRxiv - Cell Biology 2021Quote: ... clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... and once with 2 bead volumes of 1X RNase H Buffer (NEB; 50mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... for one h at 37 °C in the CutSmart™ buffer (NEB). After that ...
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bioRxiv - Molecular Biology 2021Quote: ... known standards (H. wingei and/or Yeast Ladder from New England BioLabs) were included in each CHEF gel run.
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bioRxiv - Pharmacology and Toxicology 2020Quote: rTTP1 and LTM-TPP1 were treated with Endoglycosidase H (New England Biolabs) to remove N-glycan modifications ...
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bioRxiv - Microbiology 2020Quote: ... The remaining glycopeptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc residue at the corresponding site ...
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bioRxiv - Microbiology 2022Quote: ... for 7-12 h at 37°C in CutSmart buffer (NEB, B7204S). Digested products were then visualised on a 4% NuSieve (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... Endoglycosidase H digestion was performed according to manufacturer’s instructions (New England BioLabs). Complexes were dissociated at 95°C in sample buffer containing 150 mM DTT prior to loading on a gradient SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ug of DNA was incubated with 6U RNAse H (NEB, M0297) at 37 °C for 2 h ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (1.2 μg) was treated with 2 U RNase H (NEB, M2097) per µg of gDNA for 4 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and RNase H in NEBNext® Second Strand Synthesis Reaction Buffer (NEB) at 16 ºC for three hours ...
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bioRxiv - Biophysics 2024Quote: ... The RNA:DNA hybrid was then cleaved by RNase H (NEB catalogue # M0297L) to produce the final 22 nucleotide product ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were cleaved using RNAse H (New England Biolabs, Ipswich, MA, USA) with LA121 as a specific primer for HIS3-tCYC1 ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... endoglycosidase H (500 units, 1 μl, New England Biolabs, catalog no. P0702) was added to each of the SLNYLLYVSN peptide samples ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was cleared of RNA by treating with RNase H (NEB). Half the cDNA was subjected to 2nd strand synthesis using Phusion high-fidelity DNA polymerase (NEB ...
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bioRxiv - Genetics 2023Quote: ... the hybrid was reacted with 1 μl RNase H (New England BioLabs) at 37 °C for 30 min and then heated to 90 °C for 10 min to terminate the reaction ...
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bioRxiv - Cell Biology 2022Quote: ... Endo H assay was performed following manufacturer’s instructions (New England Biolabs, P0702S). Following addition of Glycoprotein Denaturing Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... before 9 μl of 5 U/μl thermostable RNase H (M0523, NEB) were added and samples were incubated 1 h at 50 °C with 800 rpm shaking (15 s on/15 s off) ...
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bioRxiv - Molecular Biology 2023Quote: ... 9 μl of 5 U/μl of thermostable RNase H (M0523, NEB) were added and samples were incubated 1 h at 50 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 25% of the sample was used for Endo H (New England Biolabs) treatment following manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... A preheated mixture of 10 µl of RNase H (New England Biolabs) and 2 µl of RNase H Buffer (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... on DNA were performed in the buffer supplied with the commercial recombinant enzyme (New England Biolabs (NEB) dam Methyltransferase Reaction Buffer or CutSmart Buffer ...
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bioRxiv - Genetics 2021Quote: ... on DNA were performed in the buffer supplied with the commercial recombinant enzyme (New England Biolabs (NEB) dam Methyltransferase Reaction Buffer or CutSmart Buffer ...
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bioRxiv - Cell Biology 2022Quote: Recombinant condensin II holo(WT) at 500 nM was mixed with or without λ protein phosphatase (NEB) at a final concentration of 400 U/µL in 1x NEBuffer for Protein MetalloPhosphatases supplemented with 1 mM MnCl2 and incubated at 30°C for 60 min ...
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bioRxiv - Immunology 2020Quote: ... The amplified DNA was then treated with recombinant Shrimp Alkaline Phosphatase and Exonuclease 1 (New England Biolabs), and sent to ELIM Biopharm for sequencing ...