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Citations for Illumina :
1 - 50 of 53 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... nuclear genome assemblies were either generated from Illumina short-read datasets or obtained from published sources ...
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bioRxiv - Immunology 2020Quote: ... Nuclear pellets were resuspended in the presence of Tn5 transposase (Illumina) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... DNA extraction was performed using QuickExtract DNA extraction solution (Epicentre, Illumina, Madison, WI) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were treated with Nextera Tn5 Transposase (Illumina, FC-121-1030) in a 50 μl reaction for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclear pellet was resuspended in the transposase reaction mix (Illumina, Nextera Tn5 Transposase kit) and incubated for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated nuclear pellets were incubated with a 50 μl reaction of Tn5 Transposase (Illumina Tagment DNA Enzyme and Buffer Large Kit 20034198 ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting nuclear pellet was resuspended in 5 µl buffer TD (Illumina, San Diego, CA) and combined with 2.5 µl H2O and 2.5 µl Tn5 transposase (Tagment DNA Enzyme ...
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bioRxiv - Developmental Biology 2022Quote: ... and the resulting nuclear pellet was resuspended in 5 µl buffer TD (Illumina, San Diego, CA) and combined with 2.5 µl H2O and 2.5 µl Tn5 transposase (Tagment DNA Enzyme ...
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bioRxiv - Genomics 2021Quote: ... Nuclear isolation of 5×10^4 cells was followed by treatment with Nextera Tn5 enzyme (Illumina, 20034198) for 45 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The nuclear pellet was resuspended in transposition reaction mix using the Tagment DNA Enzyme and Buffer Kit (Illumina) as per manufacturer protocol ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... and percent junction nuclear were calculated from A375 RNA-seq data generated in this paper (see ‘Methods: Illumina short read sequencing analysis’ and Supplemental Table S3 for additional information) ...
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bioRxiv - Cell Biology 2023Quote: ... Extraction of FastQ files was done using bcl2fastq v2.20.0.422 (Illumina). More details about procedure and data analysis is provided in SI Appendix.
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bioRxiv - Microbiology 2020Quote: ... Inc (San Diego, CA) and genomic DNA extraction followed by Illumina sequencing (see section below) ...
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bioRxiv - Genetics 2020Quote: ... We then resuspended the nuclear pellet in 50 μl of transposition reaction mix (25 μl Buffer TD, 2.5 μl TDE1 (Illumina 15028212); 7.5 μl water ...
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bioRxiv - Cancer Biology 2022Quote: ... flow-sorted tdT+ cells (25,000 cells in duplicates) were lysed to prepare nuclear pellets which then underwent transposition with TDE1 Enzyme (Illumina, 20034197). Tagmented DNA was purified using Zymo DNA Clean and Concentrator-5 Kit (cat# D4014 ...
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bioRxiv - Molecular Biology 2021Quote: ... The crude nuclear prep was then centrifuged and resuspended in 1x TD buffer containing the Tn5 transposase (Illumina FC-121-1030). The transposition reaction was incubated at 37C for 30 minutes and immediately purified using the Qiagen MinElute kit ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was extracted using QuickExtract ™ DNA Extraction Solution (Illumina, USA), and PCR amplification using primers encompassing the CRISPR target site ...
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bioRxiv - Microbiology 2022Quote: ... Library extraction was performed with the TruSeq DNA PCR-Free kit (Illumina) and sequenced with the Illumina NovaSeq 6000 platform (Illumina).
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bioRxiv - Developmental Biology 2020Quote: ... The resulting crude nuclear pellet was then resuspended in 50 ul of tagmentation solution (1x TD buffer (Illumina, San Diego, CA 20034197), 33% PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... This nuclear pellet was used to prepare ATAC-seq libraries using the Nextera DNA Library Preparation Kit (Illumina, San Diego, CA, Cat #FC1211030). The pellet was suspended in 5 μL buffer TD before adding 2.5 μL water and 2.5 μL tagment DNA enzyme ...
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bioRxiv - Cancer Biology 2020Quote: ... and centrifuged at 500 g at 4° C to isolate nuclear pellets that were treated in 50 μL reactions with Nextera Tn5 Transposase (Illumina, FC-121-1030) for 30 min at 37° C ...
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bioRxiv - Microbiology 2022Quote: ... Gel Extraction Kit (OmegaBio-Tek, USA) and sequenced on the MiSeq platform (Illumina, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... jejuni NCTC12658 was isolated using Maxwell 16 Tissue DNA extraction kit (Promega) (Illumina sequencing) and the MagAttract HMW DNA kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... for the extraction of DNA methylation signals from scanned arrays (methylation module version 1.9.0, Illumina Inc.). Methylation data were extracted as raw signals with no background subtraction or data normalization ...
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bioRxiv - Microbiology 2023Quote: ... as well as 6 PCR negative control and 3 extraction negatives on a NovaSeq 6000 (Illumina), with 2 Gb requested per sample.
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bioRxiv - Microbiology 2022Quote: Short read sequencing from blood culture broth extractions utilised the Nextera DNA Flex Library Prep Kit (Illumina), with a modification of the starting input of 5 μL gDNA with samples >20 ng/μL ...
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bioRxiv - Immunology 2021Quote: ... Ribosomal RNA was removed from each RNA extraction using Ribo-Zero Gold rRNA Removal kit (Illumina, Cat#: MRZG12324). RNA-seq libraries were generated by TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA extraction and the sequencing library preparation for short-read 2×250 bp paired-end MiSeq (Illumina) sequencing was performed as described before (Valcek et al. ...
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bioRxiv - Genomics 2022Quote: ... To the 1.5 μl concentrated air DNA extraction we added 2.5 μl Illumina Nextera reaction buffer (FC-121-1030, Illumina), 1 μl 1 pg/μl Lambda DNA (SD0011 ...
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bioRxiv - Microbiology 2022Quote: Our existing genomics workflow (incorporating sample receipt, nucleic acid extraction, library preparation, short-read sequencing (Illumina NextSeq or MiSeq), and quality control (QC ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was purified by phenol:chloroform extraction and ethanol precipitation and used to prepare Illumina libraries using the Nextera NT library prep kit (Illumina) or analyzed using qPCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted their DNA using Promega Wizard DNA extraction kit and pooled them to an equal-molar concentration for paired-end next-gen sequencing (Illumina HiSeq ...
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bioRxiv - Genomics 2022Quote: ... The PCR products were purified with QIAquick Gel Extraction Kit and Qiagen MinElute PCR purification kits and were then sequenced on a Hiseq4000 (Illumina) with 150-bp paired-end sequencing.
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA for whole-genome sequencing (WGS) analysis was isolated directly from bacterial colonies using the Nextera DNA Flex Microbial Colony Extraction kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... followed by phenol:chloroform extraction and ethanol precipitation and RRBS libraries were prepared (Boyle et al., 2012) and run on HiSeq 2500 (Illumina) using 100 bp paired-end sequencing.
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bioRxiv - Genomics 2021Quote: ... sequencing libraries from each extraction were prepared using the DNA Prep kit and indexed using DNA/RNA UD indexes (Illumina), and subsequently subjected to paired-end sequencing on either an Illumina MiSeq® or NextSeq 2000® instrument ...
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bioRxiv - Microbiology 2020Quote: ... the 309 bp sequence of the nodD coding region was amplified by PCR from the DNA extractions and the PCR products were sequenced by Illumina MiSeq 2×250 bp technology as described in Methods S5 ...
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bioRxiv - Microbiology 2023Quote: ... We extracted gDNA with the Beckman-Coulter gDNA extraction kit as above using the Nextera Flex Library Preparation Kit (Illumina). We quantitated libraries with a Qubit fluorometer (Life Technologies Corporation ...
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bioRxiv - Molecular Biology 2023Quote: ... 11200g for five minutes prior to dividing the lysate for either nuclease foot-printing with RNaseI for generation of the ribosome profiling libraries or total RNA extraction followed by removal of rRNA with RiboZero (plant leaf kit, Illumina) for the corresponding RNA-Seq library.
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bioRxiv - Microbiology 2019Quote: ... Shotgun sequencing libraries were prepared for each DNA extraction using Illumina Nextera DNA library preparation kits (Illumina, Inc., San Diego, CA). The Massey Genome Service (Palmerston North ...
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bioRxiv - Neuroscience 2019Quote: Buccal swab and saliva samples were collected for DNA extraction followed by genome-wide genotyping using the “Global Screening Array” (Illumina, Inc). For a full description of genotyping and post-genotyping methods ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from the final Δrv3645 RLC12 library stock and library quality was validated by deep sequencing (see Genomic DNA extraction and library preparation for Illumina sequencing).
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bioRxiv - Genomics 2023Quote: ... or flash-frozen in liquid nitrogen and stored at −80°C until extraction (for both PacBio SMRT sequencing and Illumina HiSeq) from a clone of ‘Montmorency’ and an accession of P ...
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bioRxiv - Molecular Biology 2022Quote: ... were pooled and RNA extraction/purification was performed for the preparation of sequencing libraries using the Illumina Stranded mRNA Prep kit (Illumina, Cat. 20040534) and IDT Illumina RNA UD Indexes Set B (Illumina ...
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bioRxiv - Plant Biology 2021Quote: AC and SlBES1-RNAi-8 fruits at breaker and red ripe stages were collected for total RNA extraction and Illumina MiSeq library was constructed as described by manufacturer’s instructions (Illumina, San Diego, CA, USA) and then sequenced with the Illumina Miseq platform.
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bioRxiv - Plant Biology 2021Quote: ... we sequenced a subset of 12 samples (six Salix and six Xanthium from both extraction methods) with the Illumina technology (Illumina Inc., San Diego, USA). Libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (for old herbarium specimens ...
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bioRxiv - Microbiology 2020Quote: ... Five DNA extractions were performed per soil sample and pooled for metagenomic library preparation with the TrueSeq DNA kit (Illumina Inc., San Diego, CA). Sequencing was performed on the Illumina HiSeq platform to generate 6.4 billion of paired-end reads of 150bp.
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bioRxiv - Pathology 2024Quote: ... Samples were sent to LGC Genomics GmbH (Berlin, Germany) for DNA extraction and sequencing (150 bp paired-end reads on an Illumina NextSeq 500/550 platform). All DNA extraction ...
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bioRxiv - Plant Biology 2023Quote: The prediction of protein-coding potential for transcripts generated from Illumina and SMRT data was conducted by integrating CPC2 v0.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and cell surface protein libraries were sequenced on the NextSeq 550 System (Illumina).