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Citations for Illumina :
1 - 37 of 37 citations for Degassed Water since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 22.5 μl water) (Illumina Cat# 20031198) was added to the nuclear pellet ...
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bioRxiv - Systems Biology 2021Quote: ... 22 μL nuclease-free-water) (Illumina FC-121-1030 Nextera DNA library prep kit) ...
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bioRxiv - Genetics 2021Quote: ... 5 µl water) with 2.5 µl transposase (Illumina 20034197) for 30 min at 37 °C with shaking at 1000 r.p.m ...
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bioRxiv - Molecular Biology 2022Quote: ... 5.4μL nuclease-free water and 2.5μL TDE1 (Tn5 enzyme, Illumina)] followed by incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 22 μl of nuclease-free water) (#20034197, Illumina; #G9441, Promega) for incubattion at 37°C with shaking at 300 rpm for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... 1μl of nuclease-free water and 1μl of 750nM methylated Truseq adapter (Illumina). For no-heat controls ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5μL transposase and 22.5μL nuclease-free water (Nextera DNA Library Prep, Illumina #FC-121-1030) or in 50μl of tagmentation mix containing 25μL of TAPS-DMF buffer (80mM TAPS ...
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bioRxiv - Immunology 2020Quote: ... and nuclei were resuspended in 50μl of transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl]; (Illumina)) and incubated for 30min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and nuclei were resuspended in 50μl of transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl]; (Illumina)) and incubated for 30min at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Pelleted nuclei were resuspended in 25 μL Tn5 transposition mix (12.5 μL 2X Tagment DNA buffer, 1.25 μL Tn5 transposase, and 11.25 μL sterile water; Illumina) and stored in a shaking incubator at 37°C and 500 RPM for one hour ...
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bioRxiv - Genetics 2020Quote: ... and 22.5 μl nuclease-free water) (Nextera DNA Library Prep Kit (Illumina, cat. no. FC-121-1030). The transposase reaction was performed at 37°C for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030 ...
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bioRxiv - Genetics 2021Quote: ... DNase- and protease-free molecular grade water) was sent to GATC (Eurofins) for sequencing (Genome Sequencer Illumina HiSeq).
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bioRxiv - Microbiology 2020Quote: ... water (n=9) and sediment (n=9) samples were subjected to bacterial 16S rRNA amplicon profiling by Illumina sequencing ...
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed and fragmented in a single reaction (12.5 µl 2X Illumina TDE buffer, 2.5 µl 1% Tween-20, 2.5 µl 0.2% Digitonin, 5 µl water, 2.5 µl Illumina TDE1 enzyme). Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo) ...
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bioRxiv - Genomics 2021Quote: ... Beads were then re-suspended in the tagmentation mix (19 μl tagmentation buffer + 1μl Tagment DNA enzyme supplemented with 5μl nuclease-free water) from the Nextera DNA Sample Prep kit (Illumina) and incubated at 37°C for 10 minutes in a thermocycler ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl Tagment DNA Enzyme and 20 μl nuclease free water (Nextera DNA Sample Preparation Kit, Illumina, UK) for 45 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 μL ATAC reaction mix (25uL 2X TD buffer, 2.5 μL Nextera Enzyme, 22.5 μL water, Illumina). The transposase reaction was carried out at 37°C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... The nuclei pellets were resuspended in 50μl of transposition buffer (25μl of 2× TD buffer, 22.5μl of distilled water, 2.5 μl of Illumina Tn5 transposase) and incubated at 37 °C for 30 min with shaking at 1000 rpm for fragmentation ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei pellet was then resuspended in the 50ul of the transposition reaction mix (25ul Tagment DNA buffer, 2.5ul TD Tn5 Transposase enzyme, 22.5ul nuclease-free water) (Illumina; 20034197). The transposition reaction was incubated at 37°C for 30 min on a thermomixer with constant gentle shaking at 1000 rpm ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell pellet was resuspended in transposition reaction mix (12.5 μl TD-Buffer, 1.25 μl Tn5, 11.25 μl water) (Nextera DNA Library Prep Kit, Illumina, Cat# 15028212) and incubated for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... was performed by incubating beads with tagmentation buffer (12.5 µl 2 x TD buffer, 11.5 µl nuclease free water, 1µl Tn5 enzyme (Illumina #FC-131-1024)) at 37°C for 10min ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries that passed quality control were diluted to 2nM DNA content using sterile water and then sequenced on the NextSeq500 (Illumina) at a final concentration of 12pM ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extracted from sorted mouse brain hemisphere microglia was eluted in 30 μl nuclease-free water and subjected to 50 bp single-end mRNA sequencing using HiSeq 4000 (Illumina). Raw sequencing data were first evaluated by FASTQC v0.72 for quality ...
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bioRxiv - Microbiology 2021Quote: ... DNA was diluted to 0.2 ng/μL in nuclease-free water and processed for sequencing using the Nextera XT DNA Library Prep Kit (Illumina).
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bioRxiv - Systems Biology 2020Quote: ... DNA was diluted to 0.2 ng/uL in nuclease-free water and processed for sequencing using the Nextera XT DNA Library Prep Kit (Illumina).
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands between ∼210 bp and ∼280 bp (tRNAs plus adapters and barcodes) cut out and co-extracted overnight (by soaking in deionized, ultrapure water (Illumina)) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10μL 2xTagment DNA buffer and 1μL of 100-fold in molecular-grade water diluted DNA tagmentation enzyme (Illumina 20034197, lot 20464427) were added to each 1μL input ...
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bioRxiv - Microbiology 2022Quote: ... a selection of 288 Enterobacterales recovered from water (n=155) and wastewater (n=133) samples underwent paired-end short read sequencing using Illumina (Illumina, USA) NovaSeq 6000 or MiSeq platforms ...
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bioRxiv - Molecular Biology 2023Quote: ... and the nuclei resuspended in 47.5μl of Tagmentation buffer (containing 25μl of 2x Tagmentation buffer from Illumina and 22.5μl of water) before adding 2.5μl of Tn5 enzyme (Illumina Nextera kit ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030; 5μl Tn5 Transposase, Illumina Cat#FC-121-1030) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... The resulting nuclei were resuspended in 50µl of transposition reaction buffer (25µl of 2x TD buffer from the Nextera kit, Illumina; 2.5µl of Tn5 transposase enzyme from the Nextera kit, Illumina; 22.5µl of nuclease free water), and incubated at 37°C for 90 min for chromatin tagmentation ...
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bioRxiv - Genetics 2020Quote: ... and the nuclei was resuspended with Tn5 transposase reaction mix (25◻μl 2◻×◻TD buffer, 2.5◻μl Tn5 transposase and 22.5◻μl nuclease-free water) (Illumina) at 37◻°C for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... denatured using 0.1N NaOH followed by a 2min incubation @96C followed by 5min in an ice-water bath just prior to sequencing as per the Illumina protocol for a 2×301 MiSeq run (Illumina, Inc., San Diego, CA). DNA sequence reads from the Illumina MiSeq were demultiplexed and classified in the following manner ...
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bioRxiv - Molecular Biology 2022Quote: ... miRNA libraries were prepared from the isolated EV RNA and a water control using the TruSeq Small RNA Library Preparation Kit (RS-200-0012, Illumina, San Diego, CA, USA) using the manufacturer instructions with the modification of performing the adaptor ligation in the presence of 15% polyethylene glycol in order to reduce bias by driving the ligation reactions towards completion as determined by the Extracellular RNA Communication Consortium (46) ...
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bioRxiv - Microbiology 2023Quote: ... The 16S rRNA gene V4-V5 region was amplified from each water sample and sequenced using the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) (Tremblay et al. ...