Labshake search
Citations for Illumina :
851 - 900 of 1467 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... 0.1 ng of cDNA from each cell on the plate was individually tagmented using the Nextera XT kit (Illumina, San Diego, CA) in a final volume of 5 µl ...
-
Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... DNA was extracted from PBMC and full-length HLA genes were sequenced by NGS on the MiSeq platform (Illumina, San Diego, CA). FASTQ files generated by MiSeq Reporter were analyzed by NGSengine v2.16.2 (GenDX ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... DNA was diluted to approximately 2.5 ng/μL and enzymatically sheared using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, CA) at 55°C for 5 minutes ...
-
bioRxiv - Genetics 2021Quote: ... 300 ∼ 420 ng of RNA was used for E8.5 cDNA library generation with the KAPA-Stranded mRNA-seq kit (Illumina Platforms, KR0960-v5.17).
-
bioRxiv - Genomics 2021Quote: ... ~800 ng of total RNA was depleted of rRNA using the Ribo-Zero rRNA Removal Kit (Illumina, Inc., San Diego, CA, USA), sheared ...
-
bioRxiv - Genomics 2022Quote: Genomic libraries were prepared from 1 ng of high-quality genomic DNA using the Nextera DNA Flex Library Prep kit (now called Illumina DNA Prep, Illumina C.A., USA), following the manufacturer instructions ...
-
bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared from 2500 ng total RNA using the TruSeq RNA Library Preparation Kit v2 (Illumina, San Diego, CA, USA) after poly(A ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were prepared using 200 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using Qubit fluorometry and an Agilent BioAnalyzer DNA 1000 chip (Santa Clara ...
-
bioRxiv - Genomics 2024Quote: ... were used as the input material (approximately 1 ng total quantity) for preparing shotgun metagenome sequencing library with the help of Nextera XT DNA Library Prep Kit (Illumina Inc., USA). Quality of the final library was assesed using high sensitivity D1000 screen tape in 2200 TapeStation (Agilent Technologies Inc. ...
-
bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
-
bioRxiv - Cancer Biology 2024Quote: RNA-seq was performed by the Genome Sciences Laboratory at Center for Public Health Genomics with 50–550 ng total RNA (TruSeq® Stranded Total RNA Library Prep, Illumina, #20020596) on an Illumina NextSeq instrument (500/550 High Output Kit v2.5 (150 Cycles) ...
-
bioRxiv - Genomics 2023Quote: 1 ng of genomic DNA from each sample was used with Illumina MiSeq Nextera XT DNA Library Preparation Kit (Illumina, Inc., USA), the paired-end library was constructed with an insert (500 bp ...
-
bioRxiv - Microbiology 2023Quote: ... 200 ng of genomic DNA was used as input in the Illumina DNA Prep Kit following the manufacturer’s instructions (Illumina, San Diego, CA). For the in vitro and in vivo experiments (Fig ...
-
bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA libraries for shotgun metagenomic sequencing of viral concentrates were prepared using an Accel-NGS® 1S Plus DNA Library Kit (Swift Biosciences, USA) and sequenced on an Illumina MiSeq platform (Illumina, USA) using Illumina MiSeq Reagent Kit V3 (Illumina ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were diluted to a concentration of 0.2 ng/µL and sequencing libraries were prepared using the Nextera XT Library Prep protocol (Illumina FC-131-1024). M-RTPCR and library preparation was performed in duplicate for all viral RNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RNA-seq libraries were prepared starting from 200 ng of DNase-treated total RNA according to the Illumina TruSeq Stranded Total RNA protocol (Document # 1000000040499 v00, © 2021 Illumina, Inc.). The libraries qualities were assessed by running a 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies ...
-
bioRxiv - Plant Biology 2023Quote: ... Sequence libraries were prepared from a minimum of 500 ng of total RNA using the standard TruSeq Stranded mRNA library protocol (Illumina, Cat # 20020595) and sequenced on the NovaSeq 6000 S4 flow cell to produce at least 20 million 150 bp paired-end reads for each sample ...
-
bioRxiv - Biochemistry 2022Quote: The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with Illumina Nextera XT Kit (Illumina, #FC-131-1024) following the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2023Quote: ... Approximately 300 ng of RNA was used for library prep (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina) following standard kit procedure ...
-
bioRxiv - Neuroscience 2023Quote: ... libraries were constructed from 1000 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, NEB-E7560S, New England Biolabs) and the small RNA fraction sequenced (NextSeq 500/550 High Output Kit v2.5 75 Cycles ...
-
bioRxiv - Genetics 2023Quote: ... 10 ng of amplicon DNA from PCR reaction 1 was combined with 10 µL of Nextera adapter index primers (Illumina Cat#20027213) and 25 µL Kapa HiFi Hotstart ReadyMix ...
-
bioRxiv - Microbiology 2023Quote: ... The NGS library was prepared with the Nextera XT DNA Library prep kit following manufacturer’s instructions with changes (Illumina #FC-131-1096). Initially ...
-
bioRxiv - Microbiology 2024Quote: Indexed sequencing libraries were generated from 1 ng genomic DNA using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the protocol of the manufacturer ...
-
bioRxiv - Immunology 2024Quote: ... RNA libraries were generated from 200 ng RNA using the Illumina total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina, United States). In brief ...
-
bioRxiv - Genetics 2024Quote: ... 10 ng of amplicon DNA from the first PCR reaction was combined with 10 µL of Nextera adapter index primers (Illumina Cat#20027213) and 25 µL Kapa HiFi Hotstart ReadyMix ...
-
bioRxiv - Microbiology 2024Quote: ... RiboZero: rRNA was removed from 10 ng of total RNA using Ribo-Zero(TM) rRNA Removal Kit (Illumina, San Diego, CA, USA). Stranded cDNA libraries were generated using the Illumina Truseq Stranded mRNA Library Prep kit ...
-
bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 200uL of Transposition Mix (1x TD buffer containing 20 U/mL Superase-In RNase Inhibitor, 40 U/mL RNasin ribonuclease inhibitor and 1.25 µl of Illumina Tagment DNA TDE1 Enzyme per every 100 000 nuclei ...
-
bioRxiv - Immunology 2021Quote: ... DRB3/4/5 kits) for sample library preparations and ran on a Miseq sequencer (Illumina), following the manufacturer’s instructions.
-
bioRxiv - Genomics 2024Quote: We followed the general GATK version 4 Best Practices to call genetic variants from Illumina RNA-seq data ...
-
bioRxiv - Microbiology 2023Quote: ... a 4 nM library was sequenced on the Illumina Nextseq500 platform (Illumina, San Diego, CA) at the Radboudumc sequencing facility ...
-
bioRxiv - Systems Biology 2023Quote: ... allowing 4 lanes of 10x to be sequenced per NovaSeq S1 flow cell (Illumina #20028319) using a 28bp read 1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 25 mL of 2x NEBNext Ultra II Q5 Master Mix, 3 mL of 10 mM Universal PCR primer from Illumina, 3 mL of 10mM Index PCR primer and 9 mL of nuclease-free water and amplified for 10 cycles (98C for 10 s ...
-
bioRxiv - Genetics 2023Quote: ... Plasma was divided from maternal peripheral blood (5[mL) by centrifuging and then 600[μL (Ion Torrent) or 1.4 mL (Illumina CN500) plasma was used to extract cell-free DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... were prepared from HepG2 cell samples using 200–500 ng of total RNA following the manufacturer’s instructions for the TruSeq RNA kit (Illumina, San Diego, CA, USA). With this kit ...
-
bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared starting from 1000 ng of total RNA using the RNA Sample Prep Kit v2 (Illumina Inc, San Diego, CA, USA) including a poly-A selection step following the manufacturer’s instructions and sequenced as 2 x 100 nt paired-end reads using an Illumina HiSeq 2500 ...
-
bioRxiv - Microbiology 2020Quote: ... Library construction and sequencing were conducted using the Illumina Nextera NGS Library Prep Kit or the Illumina Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina NextSeq with 150bp paired-end reads ...
-
bioRxiv - Developmental Biology 2020Quote: ... libraries were prepared from 1 ng of cDNA using the Nextera® XT DNA Library Preparation Kit (Illumina Inc., San Diego, CA USA) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... 500 ng of total RNA was used for each RNA-seq library preparation using a TruSeq Standard mRNA kit (Illumina, San Diego, CA) as described in our previous publications (Khristi et al. ...
-
bioRxiv - Genomics 2019Quote: ... The capture libraries were sequenced on MiSeq (9 samples per run) or HiSeq (96 samples per run) NGS platforms (Illumina, San Diego, CA) as previously described24 ...
-
bioRxiv - Genomics 2019Quote: RNA-seq libraries were prepared using 1000 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, CA, USA) on a Sciclone liquid handling robot (PerkinElmer ...
-
bioRxiv - Cancer Biology 2019Quote: ... mRNA sequencing libraries were generated from 50 ng total RNA using the Illumina Neoprep TruSeq stranded mRNA library prep kit (Illumina, NP-202-1001). RNA libraries were sequenced paired-end (2 × 75 bp ...
-
bioRxiv - Plant Biology 2019Quote: ... DNA fragments underwent an NGS library preparation procedure consisting in end repair and methylated adaptor ligation using the TruSeq DNA Sample Preparation kit (Illumina, San Diego, CA). Adapter-ligated fragments ranging from 200 to 300 nucleotides were isolated by agarose gel electrophoresis (Bio-Rad ...
-
bioRxiv - Plant Biology 2020Quote: ... RNA-seq libraries were prepared using 1000 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) on a Sciclone liquid handling robot (PerkinElmer ...
-
bioRxiv - Cancer Biology 2021Quote: ... 500 ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
-
bioRxiv - Neuroscience 2020Quote: ... Library preparation was performed by capture of sequence-specific coding RNA using 40 ng of total RNA and the TruSeq RNA Access Library Prep Kit (Cat No RS-301-2001, Illumina, San Diego, USA). Libraries were sequenced as paired-end 68 bp reads on a HiSeq2000 (Illumina ...
-
bioRxiv - Neuroscience 2019Quote: ... and libraries were constructed using 600 ng of total RNA from each sample and the TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following manufacturers suggested protocol ...
-
bioRxiv - Microbiology 2020Quote: ... The consortium metagenomic library was prepared with 200 ng of starting material using the TruSeq DNA Nano kit (Illumina, San Diego, CA, USA) and paired-end sequenced (2×100 bp ...
-
bioRxiv - Microbiology 2021Quote: ... The sequencing amplicon pools were diluted to 0.2 ng/µl and tagmented with Nextera XT library prep kit (Illumina, Cat#FC-131-1024). Nextera libraries were dual-barcoded and sequenced on an Illumina NextSeq1000 instrument ...