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Citations for Bioline :
1 - 37 of 37 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The final volume contained 1× SensiMix (Bioline, London, UK), 800 nM of each primer and 200nM of each probe and the PCR cycling conditions included an initial denaturation at 95°C for 10 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated small RNA (bound by AGO4) was released from beads and isolated by TRIsure (Bioline) extraction ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplification was performed in 25µl volumes with 2Χ Ready-mix (Bioline, UK) (volume 12.5µl ...
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bioRxiv - Molecular Biology 2024Quote: ... A volume of GRASPS buffer A1 containing 0.16 U/μl RNase inhibitor (Bioline), 2 mM PMSF and cOmplete™ EDTA-free Protease Inhibitor Cocktail corresponding to approximately three times the volume of the cell pellet (600 μl ...
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bioRxiv - Microbiology 2022Quote: ... PCR used a total volume of 20 µL with 10 µL BioMix (BioLine, UK) 1 µL of each primer (10 µM) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PCR used a total volume of 20 μL with 10 μL BioMix (BioLine, UK) 1 μL forward primer (10 μM) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reaction volumes are as follows: 12.5 μL MyTaqTM Master Mix (Bioline, United Kingdom), 10 μL molecular-grade water (G-Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... A total reaction volume of 20 μL containing 1x SensiFAST SYBR Lo-ROX mix (Bioline), 200 μM of each forward and reverse primer ...
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bioRxiv - Pathology 2023Quote: ... All real-time PCR reactions were performed in 20 μL volumes containing 10 μL Immomix (Bioline) and 5 μL of DNA template ...
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bioRxiv - Pathology 2023Quote: ... Reactions for uniplex and duplex assays were performed in 20 μL volumes containing 10 μL Immomix (Bioline) and 5 μL of DNA template ...
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bioRxiv - Cancer Biology 2020Quote: The assay consists of 10 μl of reaction volume including 5 ul of 2X buffer (Bioline, Bio-11060), 2 ul of Primer/probe mix in 1xTE with final concentration of 100 nM-600 nM of primers and 50 nM – 500 nM of probes ...
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bioRxiv - Genetics 2022Quote: ... the multiplex-PCR was performed in a total volume of 25 μl mix solution containing 12.5μl MyTaq HS Red Mix (Bioline), 40ng of genomic DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplifications were carried-out in total volume of 10 µL containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of each primer set (dhfr-F/R and 529rpe-F/R) ...
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bioRxiv - Plant Biology 2020Quote: ... Final elution was performed with 40 µl DNA/RNAase-Free water supplied and the eluted RNA was subsequently quantified using a ND-1000 Spectrophotometer (NanoDrop Technologies) and quantified on a 1% (w/v) agarose gel (Bioline) by electrophoresis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were carried out in a volume of 15 μL including 0.25-1.0 U of MyTaq™ HS DNA polymerase (Bioline), 2 μL of MyTaq™ 5x reaction buffer (Bioline) ...
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bioRxiv - Zoology 2019Quote: ... The amplification was performed in a final volume of 12.5 μl using the SensiMix SYBR Low-ROX Kit (Bioline) with each primer at 200 nM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Reactions were performed in a total volume of 25 μl containing 12.5 μl 2 x MyTaq Red (Bioline, UK), 0.5 μl of each 10 μM primer stock ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of each forward primer 341F 5’-NNNNNNNNNNTCCTACGGGNGGCWGCAG and reverse primer 785R 5’-NNNNNNNNNNTGACTACHVGGGTATCTAAKCC in 20 μL volume of 1 x MyTaq buffer containing 1.5 units MyTaq DNA polymerase (Bioline) and 2 μl of BioStabII PCR Enhancer (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... with total reaction volume of 10 μl containing 5 μl Rotor-Gene SYBR® Green PCR Kit (Bioline, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleic acids were precipitated with three volumes of ethanol in the presence of a co-precipitating agent (PINK, Bioline) and dissolved in 20 μl of milliQ-grade water.
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bioRxiv - Genetics 2020Quote: The optimized final reaction conditions were performed in a final volume of 25 μl containing 1.6X NH4 buffer (Bioline); 4 mM MgCl2 (Bioline) ...
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bioRxiv - Microbiology 2019Quote: ... was used for preparation of cDNA-libraries which were amplified in a volume of 100 µl for 15 cycles using MyTaq (Bioline) and standard Illumina primers ...
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bioRxiv - Molecular Biology 2022Quote: RT-qPCR was performed in 20.0 µl final reaction volume containing 10.0 µl of 2X SensiMix SYBR® No-ROX master mix (Bioline, UK), 0.3 µM of forward and reverse primers and 1.0 µl of cDNA ...
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bioRxiv - Microbiology 2023Quote: ... The reactions (10 μL total volume) contained 5 μL of 2 × SensiFAST™ SYBR No-ROX Kit (Bioline, London, UK), 0.2 μM of each arsM gene primer ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was amplified on quantitative PCR in a total volume of 5 μl with SensiFAST SYBR® No-ROX Kit (Bioline) and specific primers on a LightCycler 480 (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reactions (a total volume of 50μl reaction mixture) each consisted of consisting of 10 μl Buffer of 5x MyTaq Reaction Buffer (Bioline, with dye), 0.75μl of each the primers intI1 ...
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bioRxiv - Microbiology 2020Quote: ... 250-1,000 ng of extracted RNA was converted to cDNA in a reaction volume of 20 μl using the Tetro cDNA synthesis kit (BIO-65043, Bioline, London, UK). The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001 ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR primer concentration was 500 nM in a volume of 10 μl with the SensiFast SYBR No-ROX Kit (Bioline, London, UK). The following amplification program was used ...
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bioRxiv - Microbiology 2020Quote: ... we used a primer concentration of 500 nM in a final volume of 10 μl with the SensiFast SYBR No-ROX Kit (Bioline, London, UK). The amplification conditions were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was prepared from 300 ng of RNA in a total reaction volume of 20 µl using the Sensi-FAST cDNA synthesis kit (Bioline, BIO-65053). RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline ...
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bioRxiv - Cancer Biology 2020Quote: Real-time RT-PCR reactions were performed in 20ml volumes with 10 ml of SensiFAST SYBR Lo-ROX Kit (Bioline, Taunton, MA) 2ml of cDNA template and 0.5ml each of the forward and reverse primers of the gene of interest (GOI) ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions were set up in total volumes of 10 µl each, containing 5× MyTaq reaction buffer (5 mM dNTPs, 15 mM MgCl2, stabilizers and enhancers) (Bioline, London, UK), 2 µM of each primer ...
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bioRxiv - Microbiology 2020Quote: All PCRs were carried out in 25 μl reaction volumes containing 0.05 units of MyTaq™ DNA Polymerase (Bioline Laboratories, London, UK), 1x MyTaq™ Mix (Bioline ...
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bioRxiv - Genomics 2023Quote: ... Samples were prepared for qPCR in technical duplicates in 10-μl reaction volumes using SensiFAST SYBR Lo-ROX 2× Master Mix (Bioline, BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2 μM and cDNA diluted at 1:20 by volume ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for qPCR in technical triplicates in 5 µL reaction volumes using SensiFAST SYBR Lo-ROX 2X master mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2 µM ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2LµM and cDNA diluted at 1:3 ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9 target sites within the AAVS1 locus were PCR amplified in a 25 or 50 μL reaction volume per sample using high-performance RANGER PCR Mix (Bioline USA, Inc., Memphis, TN, USA) or PlatinumTM SuperFiTM PCR Master Mix (Thermo Fisher Scientific ...