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Citations for Bioline :
1 - 50 of 159 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli DH5α (Bioline) was used for cloning and E ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli DH5α (Bioline) were used for cloning and grown in lysogeny broth (LB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sensifast Hi-Rox SyBr kit (Bioline) was used to perform the RT-qPCR on a StepOnePlus system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... using SensiFAST SYBR Hi-ROX (Bioline). A total cDNA concentration of 100 ng in combination with 200 nM of dapE oligonucleotide per reaction (Supplementary Table 5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... qRT-PCR was carried out on 1:2 diluted cDNA on an Applied Biosystems StepOnePlus system using a Sensifast Hi-Rox SYBR kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Biophysics 2021Quote: ... coli α-Select (Bioline, UK) was used for general plasmid storage and propagation ...
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bioRxiv - Developmental Biology 2019Quote: ... with SensiMix SYBR Hi-ROX Kit (Bioline) following manufactures instructions ...
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bioRxiv - Microbiology 2020Quote: ... The SensiFAST SYBR Hi-ROX kit (Bioline) was used for qPCR reactions ...
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bioRxiv - Microbiology 2021Quote: ... or SensiFAST SYBR Hi-ROX kit (Bioline). Reactions were run using Applied Biosystems 7500 fast RT-PCR machine (Thermofisher ...
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bioRxiv - Synthetic Biology 2020Quote: ... the SensiMix SYBR Hi-ROX (Bioline Reagents) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... and SensiFAST SYBR Hi-ROX mix (Bioline). Total reaction volume was 10 µl with 150 nM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... coli Silver α select cells (Bioline) were used for cloning and were grown in LB medium at 37°C with 25 μg/ml kanamycin or 10 μg/ml tetracycline when appropriate.
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bioRxiv - Synthetic Biology 2020Quote: ... Escherichia coli α-Select (Bioline, UK) was used as a host for plasmid propagation ...
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bioRxiv - Microbiology 2020Quote: ... coli α-select silver efficiency (Bioline) was used to manipulate the pGEM-TEasy library of gene cassettes ...
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bioRxiv - Cell Biology 2021Quote: ... SensiFAST™ SYBR® Hi-ROX Kit (Bioline) in QuantStudio™ 5 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The SensiFAST SYBR® Hi-ROX kit (Bioline) was used for qRT-PCR that was performed with the QuantStudio 3 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... using the SensiFAST SYBR Hi-ROX kit (Bioline). Predesigned KiCqStart SYBR green primers for the analyzed cytokines ...
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bioRxiv - Neuroscience 2019Quote: ... A nested PCR (Table S2) using MyTaq™ HS Red Mix (Bioline; United States) was necessary for a sufficient amplification of a 920 bp region flanking the gRNA1 target site ...
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bioRxiv - Immunology 2021Quote: ... and assessed by qPCR (SensiMix SYBR Hi-Rox, Bioline). Expression levels of each gene were normalized against GAPDH expression and fold change was calculated using the 2-ΔCT or 2-ΔΔCT equations [54] ...
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bioRxiv - Cell Biology 2023Quote: ... assays and SensiFAST™ Probe Hi-ROX Kit (Bioline) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... assays and SensiFAST™ Probe Hi-ROX Kit (Bioline) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... cells were harvested and resuspended in 800 μl TRIsure (#BIO-38032; Bioline) and stored at -80 °C until further use ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL was used as a template in 20 μL qPCR reactions containing 10 μL SensiFAST™ Probe Hi-ROX (Bioline, Cat. No. BIO-82020), 200 nM probe ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed using SensiFast SYBR Hi-ROX kit (Bioline) on CFX96 Touch Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... qPCR reactions were performed using SensiFAST SYBR Hi-ROX (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... 125nM) and SensiFAST Probe Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s instructions in a 20μl reaction with 5μl sample ...
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bioRxiv - Microbiology 2020Quote: ... The Sensi-FAST SYBR Hi-ROX One-Step Kit (Bioline) was used for quantitative real time PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2021Quote: ... with Sensi-FAST Probe Hi-ROX qPCR kit (Bioline, 82005) (8).
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bioRxiv - Developmental Biology 2023Quote: ... Reactions were performed with SensiFAST SYBR Hi-ROX kit (Bioline), and all samples and negative controls were run in technical duplicate on an Applied Bioscience StepOnePlus qPCR machine (Life Technologies).
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bioRxiv - Genomics 2021Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepared for plasmid extraction using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped using a midiprep kit ...
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bioRxiv - Genetics 2023Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped with a midiprep kit ...
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bioRxiv - Physiology 2023Quote: ... 800 nM of forward and reverse primers and SensiFAST SYBR Lo-ROX (Bioline). We ran gene-specific qPCR plates and each sample was analysed in duplicate ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using SensiFast SYBR Hi-ROX qPCR kit (BioLine). rp49 was used as a housekeeping gene for ΔΔCt calculations ...
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bioRxiv - Microbiology 2019Quote: ... The SensiFAST SYBR Hi-ROX kit (Bioline United Kingdom, BIO-92020) and custom gene-specific primer sets were used to assay 20 ng DNA per reaction for HCMV gB (UL55 ...
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bioRxiv - Microbiology 2020Quote: ... in 20μl reactions using the SensiFAST Probe Hi-ROX Kit (Bioline) (cycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed using SensiFAST™ SYBR Hi-ROX Kit (Bioline). rp49 was used as a housekeeping gene for ΔCt calculations ...
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bioRxiv - Microbiology 2020Quote: ... coli α-Select competent cells were obtained from Bioline Reagents Ltd (UK) ...
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bioRxiv - Biochemistry 2022Quote: ... coli α-select chemically competent cells (Bioline BIO-85026) and colonies were selected on YT glucose plus 50 mg/mL spectinomycin HCl (Sigma-Aldrich S9007) ...
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bioRxiv - Immunology 2019Quote: ... qPCR was then performed using the SensiFAST SYBR Hi-Rox kit (Bioline) and a StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... Resulting cDNA was amplified with the SensiFAST Probe Hi-ROX Kit (Bioline). The fold change in expression were calculated by the ΔΔCT method using snoRNA202 and U6 RNA as reference small RNAs in mouse and human cells ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed using the SensiFAST SYBR Hi-ROX kit (Bioline) and an AriaMX Real Time PCR system (Agilent Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed with the SensiFAST SYBR Hi-ROX Kit (Bioline) using a StepOnePlus 96-well plate reader (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... in 20μl reactions using the Sensi FAST Probe Hi-ROX Kit (Bioline) (cycling conditions ...
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bioRxiv - Microbiology 2019Quote: ... coli (alpha-select competent cells, Bioline or XL1-Blue, Stratagene) and plasmids were purified using Zyppy Plasmid Kits (Zymo Research Corporation) ...
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bioRxiv - Molecular Biology 2020Quote: ... 800 ng of RNA were used for reverse transcription with the SensiFASTTM cDNA Synthesis kit (Bioline) according to the standard protocol.
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bioRxiv - Immunology 2019Quote: ... Quantitative-PCR was set up using the SensiFAST SYBR Hi-ROX kit (Bioline) on a StepONE system (Applied Biosytems) ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 µL SensiFAST(tm) Probe Hi-ROX Kit master mix (Bioline, Tauton, MA), 1.25 µL each of Cx ...
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bioRxiv - Microbiology 2019Quote: ... using the SensiMix™ SYBR® Hi-ROX Kit (Bioline, Cat#QT605-05).