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Citations for GE Life Sciences :
1 - 50 of 281 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... with 100nM miR scrambled or miR-181a mimic (100nM; GE Healthcare) (Soriano-Arroquia et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Truncated mouse RANKL transcript (encoding amino acids 137-316 of mouse RANKL) was PCR amplified (forward primer: CACCCCCGGGCAGCGCTTCTCAGGAGCT, reversed primer: GAGACTCGAGTCAGTCTA TGTCCTGAAC) and then cloned into pGEX-5X-2 vector (GE Healthcare) between SmaI and XhoI sites ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated using Illustra RT-PCR ready-to-go beads (GE Healthcare) in a two-step reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding ORF regions were PCR-amplified from the parent plasmid pcDNA3-Flag-RBM17 with specific primer sets (Supplementary Table S2) and subcloned into the pGEX6p2 vector (GE Healthcare Life Sciences). To construct the series of SAP30BP and SAP30 expression plasmids (pcDNA3-Myc-SAP30BP ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding ORF regions were PCR-amplified from HeLa cells cDNA with specific primer sets (Supplementary Table S2) and subcloned into the pcDNA3-Myc or pGEX6p2 vectors (GE Healthcare Life Sciences). Overlap extension PCR was performed to induce the mutation in the ULM of pcDNA3-Myc-SAP30BP (pGEX6p2-SAP30BP/ULMmt).
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bioRxiv - Plant Biology 2021Quote: ... nested PCR was performed using primers listed in Supporting Information Table S1 and Ready-ToGo beads (illustra PuReTaq PCR Beads, GE Healthcare). In the first round ...
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bioRxiv - Genomics 2021Quote: ... After capillary transfer in 10× SSC onto a Hybond N+ membrane (GE Healthcare), RNA was UV-crosslinked and stained with methylene blue to visualise ribosomal RNA bands ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... sections were washed in 4X SSC and RNA digested with RNase A (GE Healthcare) at 0.02 mg⁄mL in an appropriate buffer (0.5M NaCl ...
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bioRxiv - Molecular Biology 2022Quote: cDNAs were PCR-amplified using corresponding primers (Supplementary Table S3) and cloned into a modified pGEX4T1 vector (GE Healthcare) encoding the TEV protease cleavage site after GST and into the vector derived from pACYC and pET28a(+ ...
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bioRxiv - Plant Biology 2023Quote: ... The DNase I-treated RNAs were reverse transcribed using Ready-to-Go RT-PCR beads (GE Healthcare Life Science) and random oligomers ...
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bioRxiv - Genetics 2021Quote: ... DNA was transferred in 2 x SSC to positively charged nylon membranes (GE Healthcare Amersham Hybond-N+) and was then immobilized by ultraviolet cross-linking (1200 J) ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized from 2 µg of total RNA using Ready-to-Go RT-PCR beads (GE healthcare) and an oligo(dT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products amplified with primers Aeatro-Nix-F1/R1 were purified with GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and cloned into pJET1.2/blunt cloning vector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products amplified with primers Aeatro-Nix-F2/R2 were purified with GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) and directly sequenced.
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed (2x SSC/0.1% SDS) and then dried for exposure on a phosphorimager screen (GE Healthcare) and scanned with a Typhoon 9400 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The molecular weight of AmvR in solution was estimated by comparing its elution volume to those of a set of molecular weight standards (HMW and LMW calibration sets, GE Healthcare).
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bioRxiv - Plant Biology 2024Quote: ... the first cDNA strand was generated from 1 µg of the RNA using Ready-to-Go RT-PCR beads (GE Healthcare) and random oligomers ...
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bioRxiv - Genomics 2021Quote: ... coupling the resulting probe set to Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... The data sets were deconvolved with softWoRx (Applied Precision). 3D data sets were converted to Quick Projections in softWoRx. ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was then transferred by capillarity in SSC 20X onto an Amersham Hybond-X membrane (GE healthcare RPN203). For TTAGGG repeats probe the 800 bp EcoRI fragment of the Sty11 plasmid (a gift from Titia de Lange ...
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was washed in 0.1% SDS/0.1X SSC and hybridized DNA was visualized and quantitated by phosphor-imaging on a Typhoon Scanner (GE Healthcare). The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Cell Biology 2020Quote: ... Deconvolution microscopy (DeltaVision RT; Applied Precision) was performed using a 100x 1.40 NA Plan Apo objective and filter set (Sedat Quad ...
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bioRxiv - Molecular Biology 2019Quote: ... Gel was processed for Southern blotting: DNA was transferred on nylon membrane by passive transfer in 10×SSC buffer (Amersham Hybond XL, GE Healthcare). Membranes were UV-cross-linked and hybridized overnight with 32P-labeled single-stranded DNA probes at 42 °C ...
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bioRxiv - Immunology 2021Quote: ... Whole blood was collected 25 to 39 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Microbiology 2020Quote: ... the cDNA obtained in the section “Realtime and Endpoint RT-PCR detection of deltaviruses from animal specimens” was amplified using illustra GenomiPhi V2 Kit (GE healthcare; Chicago, IL, USA). The amplified DNA was then purified with innuPREP PCRpure Kit (Analytik Jena ...
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bioRxiv - Immunology 2020Quote: ... Whole blood was collected 31 and 32 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Cancer Biology 2020Quote: ... set up in an AKTA start system (GE Healthcare Life Science) and eluted as 1 ml fractions in PBS (Gibco® ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... All images were acquired by using the Deltavision set-up (GE Healthcare) with an inverted Olympus IX71 microscope ...
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bioRxiv - Neuroscience 2020Quote: ... a PCR product of the intracellular domain flanked by BamHI and XhoI restriction sites was created from the equivalent full-length construct (forward primer catcatggatcctacaagcgggaccggcgcc; reverse primer catcatctcgagctatacccgagtggtggagtg) and subcloned into pGEX4T3 (GE Healthcare). GFP-tagged Gephyrin-FingR was a gift from Don Arnold (Addgene plasmid #46296 (Gross et al. ...
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bioRxiv - Bioengineering 2023Quote: ... We then pooled all oligonucleotides and coupled the set to Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was performed on an Äkta primer (GE Healthcare) using a gradient from 0-100% Ni-elution buffer (50mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: Human Custom ON-TARGET plus siKSRP or mimic miRs were resuspended in siRNA dilution buffer (Dharmacon, GE Healthcare, Chalfont-St-Giles, United Kingdom) to a 20 µM stock solution ...
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bioRxiv - Cell Biology 2021Quote: A DeltaVision RT system (Applied precision, Olympus IX71 based) equipped with the Photometrics CoolSnap HQ camera (Roper Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... both sets of reactions were purified using Microspin Illustra G-25 columns (GE Healthcare) and Zeba spin desalting columns (7K MWCO ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were developed using ECL primer solution (GE healthcare RPN2236). The primary antibodies used in this study are the followings ...
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bioRxiv - Cancer Biology 2019Quote: ... Images were acquired on a DeltaVision RT system (Applied Precision) with a ×100/1.40NA UPlanSApo objective (Olympus ...
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bioRxiv - Microbiology 2019Quote: ... on a DeltaVision®RT microscope (Applied Precision, Washington, USA) as described by Domínguez-Cuevas et al ...
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bioRxiv - Cancer Biology 2019Quote: ... Images were acquired on a DeltaVision RT system (Applied Precision) with a ×100/1.40NA UPlanSApo objective (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
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bioRxiv - Systems Biology 2020Quote: ... we pooled their complementary oligos and coupled the probe set to either Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... The column was calibrated using a set of gel filtration markers (low range, GE Healthcare), including Ovalbumin (43.0 kDa) ...
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bioRxiv - Genomics 2021Quote: ... two sets of 20 µL of Protein A or Protein G Sepharose beads (GE Healthcare) were washed three times with nuclear lysis buffer for immunoprecipitation with antibody raised in guinea pig or in rabbit respectively ...
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bioRxiv - Systems Biology 2021Quote: ... We then pooled each gene’s complementary oligos and coupled the set to Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies ...
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bioRxiv - Systems Biology 2023Quote: ... We then pooled each gene’s complementary oligos and coupled the set to Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... We then pooled each gene’s complementary oligos and coupled the set to Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies ...