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Citations for GE Life Sciences :
1 - 50 of 143 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Transfection efficiency was determined using the fluorescently-labelled siGLO RISC-free control siRNA (GE Healthcare) and miRNA and/or mRNA expression was assessed afterwards.
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bioRxiv - Cell Biology 2021Quote: ... Device control utilized softWoRx 4.1.0 software (Applied Precision). Images were acquired using a 60x 1.4 NA Plan Apochromat oil immersion lens (Olympus ...
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bioRxiv - Biochemistry 2023Quote: ... and UNICORN Control Software version 5.11 (GE Healthcare) were used for all purifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Rap1-GFP and Zip1-GFP were captured using a computer-assisted fluorescence microscope system (DeltaVision; Applied Precision). The objective lens was an oil immersion lens (100× ...
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bioRxiv - Cell Biology 2022Quote: ... Dharmacon DharmaFECT 1 transfection reagent (GE Healthcare, Manchester, UK) was used following the manufacturer’s siRNA transfection protocol with slight modification ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA transfections were performed using Dharmafect-1 (GE Healthcare) and 80nM of indicated siRNA ...
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bioRxiv - Cell Biology 2020Quote: ... or as a control Sepharose without IgG (GE Healthcare) for 3 hr at 4 °C rotating ...
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bioRxiv - Cell Biology 2023Quote: ... and RNAi negative control were purchased from GE Healthcare Dharmacon ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Physiology 2020Quote: ... After washing calmodulin and control Sepharose 4B beads (GE Healthcare) twice with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... hTert-RPE1 eGFP-HURP and hTert-RPE1 Centrin1-GFP/GFP-CENPA cells were recorded under same conditions using a Olympus DeltaVision wide-field microscope (GE Healthcare, Switzerland) equipped with a eGFP/RFP filter set (Chroma) ...
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bioRxiv - Cancer Biology 2021Quote: ... siRNA and respective siRNA control were purchased from Dharmacon (GE Healthcare) and used at a final concentration of 25nM ...
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bioRxiv - Cell Biology 2020Quote: ... Control siRNA (negative) was obtained from GE Healthcare (D-001810-10).
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed 48h post-transfection with mammalian lysis buffer (GE Healthcare), then the luciferase substrate (Promega ...
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bioRxiv - Biophysics 2023Quote: ... transfection and enhancement using Hyclone Cell Boost 1 (GE Healthcare/Cytiva, SH30584.02) as described previously(32 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP nanobody covalently coupled to NHS-agarose (GE Healthcare) and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... using a DeltaVision climate control unit (37°C, 5% CO2) (Applied Precision). The kinetics of pre-stress followed by treatment was analysed from images captured at 2 min intervals for 100 min ...
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bioRxiv - Microbiology 2020Quote: ... We isolated C1 and control donor PBMCs by Ficoll-Plaque (GE Healthcare) density centrifugation.
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bioRxiv - Microbiology 2022Quote: ... control or mutant trophozoites were rinsed once in PBS (GE Life Sciences) and twice in serum free TYI-S-33 medium (SFM) ...
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bioRxiv - Biochemistry 2020Quote: ... The standard single-cycle kinetics method (Biacore T200 Control Software, GE Healthcare) was used ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting data were fitted with Biacore T200 Control Software (GE Healthcare) using a kinetic global fit 1:1 binding model ...
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bioRxiv - Microbiology 2019Quote: ... Arf1 and the control siGENOME nontargeting siRNA were purchased from GE Healthcare Dharmacon (Lafayette ...
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bioRxiv - Microbiology 2020Quote: ... 48 hours post-transfection cells were lysed with mammalian lysis buffer (GE Healthcare), then luciferin (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... siRNAs were transfected with DharmaFECT transfection reagent (#T-2001-02, GE Healthcare Dharmacon) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... Most transfections used tetracycline-screened HyClone Fetal Bovine Serum (FBS) (SH30070.03T; GE Healthcare). This enabled a faster cell recovery after transfection than with constructs using phleomycin selection that were originally used in cell lines and appeared somewhat less stable during subculture ...
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bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 4 μL of DharmaFECT1™ (GE Healthcare, UK), 144 μL of 1 μM siRNA (35 nM final concentration ...
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bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 0.1 μL of DharmaFECT1™ (GE Healthcare, UK), 3.6 μL of 1 μM siRNA (35 nM final concentration ...
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bioRxiv - Microbiology 2019Quote: ... GFP and Bocillin were detected using a Typhoon FLA950 (GE Healthcare). For GFP ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... targeting siRNA and non-targeting scramble control siRNA were purchased from GE Healthcare (mouse Dnmt1 siRNA-SMARTpool (L-056796-01) ...
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bioRxiv - Microbiology 2020Quote: ... 1D gel electrophoresis analysis was performed with Ettan IPGphor II control software (GE Healthcare). For 2D gel electrophoresis ...
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bioRxiv - Bioengineering 2023Quote: ... Cell cultures were controlled using the UNICORN™ system control software (GE Healthcare, USA). Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... or the respective negative controls (non-targeting small interfering RNA, siCTR, GE Healthcare Dharmacon) using Lipofectamine RNAiMAX Reagent (Thermo Fisher Scientific #13778-150 ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Genetics 2020Quote: Images of ced-1::gfp were analyzed using softWoRx software (Applied Precision), and engulfed nuclei were scored only if there was complete engulfment.
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bioRxiv - Biochemistry 2021Quote: ... GFP images were taken on an Amersham Typhoon Biomolecular Imager (GE Healthcare) with a Cy2 525BP20 filter or on a ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... DC control RNA was then purified using G-50 columns (GE healthcare, 25-5330-01). All samples were then prepared for sequencing using the randomer library prep workflow protocol described in Smola et al ...
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bioRxiv - Biochemistry 2020Quote: ... GFP nanobody was coupled to CNBr-activated Sepharose Fast Flow beads (GE healthcare) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... LOO10-GFP was refolded by desalting on an Illustra NAP5 column (GE Healthcare) equilibrated with assay buffer 50 mM Hepes ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... siGENOME Control Pool Non-Targeting #2 (cat# D-001206-14-20) was purchased from GE Healthcare. siRNA transfection was performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Labeled □29 and DynA-GFP were visualized on a Delta Vision Elite (GE Healthcare) equipped with an Insight SSI illumination (Insight Lighting ...
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bioRxiv - Neuroscience 2021Quote: ... The anti-GFP nanobody was coupled to Glutathione Sepharose™ 4B beads (GE Healthcare) and incubated with lysates from transfected HEK 293T cells for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-CENH3 or CENH3 (by immunostaining) was measured using Softworx Explorer (Applied Precision Inc.). By scanning through the z-axis for each 3-D image ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biophysics 2023Quote: ... Anti-GFP nanobodies were conjugated with CNBr Sepharose beads (GE Healthcare, #17-0430-02). The resin was added to the cleared supernatant at a ratio of 1 ml of resin per 10 g of cell powder and incubated at 4 °C for 3 h with gentle shaking ...
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bioRxiv - Microbiology 2019Quote: ... Cells were imaged 24 hours post-transfection on the DeltaVision LIVE high resolution deconvolution microscope (GE Healthcare) using the 60X/1.4 Plan-Apo NA oil objective (Olympus) ...
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bioRxiv - Cell Biology 2021Quote: ... mCherry filter sets and a CoolSNAP HQ2 camera (Roper Scientific) under the control of SoftWorx (Applied Precision). 37°C and 5% CO2 were maintained in a TOKAI Hit stage incubator ...
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bioRxiv - Cell Biology 2021Quote: ... mCherry filter sets and a CoolSNAP HQ2 camera (Roper Scientific) under the control of SoftWorx (Applied Precision). Live cells were imaged using an Olympus 40x UPlanFL N Oil NA1.3 objective in imaging medium (Leibovitz’s L-15 medium (Gibco™ 21083027 ...