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Citations for GE Life Sciences :
1 - 50 of 214 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and HyClone RNase free water was obtained from GE Healthcare Cell Culture.
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bioRxiv - Biophysics 2021Quote: ... the mRNA was dissolved in HyClone HyPure Molecular Biology Grade RNase free water (GE Healthcare). DMEM cell culture media with high glucose ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Samples were first fully submerged in 3 mL of Ficoll-Plaque Plus endotoxin-free water (GE Healthcare, Uppsala, Sweden) in either a 6-well culture plate or 3-mL test tube for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: Inducible Lentiviral Nuclease hEF1α-Blast-Cas9 (GE Healthcare) was packaged into lentivirus by using MISSION Lentiviral Packaging Mix (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The labelled probes were then diluted in 40 µl of water and separated from free [γ-32P]dATP by gel filtration (Illustra Microspin columns; GE Healthcare). Dried membranes were pre-hybridized in 8 ml of pre-warmed ExpressHyb buffer (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... HyClone water (GE Healthcare) was used throughout.
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bioRxiv - Biochemistry 2022Quote: ... 1 mM CaCl2 and 10 µg/mL micrococcal nuclease (GE Healthcare) were added and incubated for 12 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Hyclone water (GE Healthcare Life Sciences) was used in all assays ...
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bioRxiv - Molecular Biology 2023Quote: ... fractions containing the nuclease were applied to a Superdex200 Increase 10/300 GL (GE Healthcare) column equilibrated with a buffer containing 50 mM Tris–HCl (pH 7.5 at room temperature) ...
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bioRxiv - Biochemistry 2020Quote: ... The nuclease substrate was separated from unincorporated dNTPs using an illustra ProbeQuant G-50 micro column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with HyClone Water (GE Healthcare Life Science) + 2% PFA for 20 min on ice ...
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bioRxiv - Pathology 2020Quote: ... free TPEN and free Zn were further removed by gel filtration on PD MidiTrap G-10 (GE Healthcare) equilibrated with 40 mM of the metal-free HEPES-KOH ...
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bioRxiv - Developmental Biology 2020Quote: ... Phenol red free DMEM (GE Healthcare/Cytiva, SH30284.01) or RPMI-1640 (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... cDNA was exchanged into water using G-50 columns (GE Life Sciences) the volume increased to 68 μl using nuclease free water ...
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bioRxiv - Cell Biology 2021Quote: ... water-swollen thiopropyl sepharose 6B beads (GE Healthcare Life Sciences, Mississauga, ON) were added and samples were incubated 2 hrs at RT on rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... Water-swollen thiopropyl sepharose 6B beads (GE Healthcare Life Sciences, Mississauga, ON) were added and samples were incubated 2 hrs at RT on rotating wheel ...
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bioRxiv - Molecular Biology 2021Quote: ... The oligomers were prepared by diluting the DMSO stock with serum-free and phenol red-free DMEM (GE Life Sciences #SH30284.01) and incubating at 4°C for 24 hours without shaking ...
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bioRxiv - Genomics 2020Quote: ... Ribosomes were treated with 60 U TruSeq Ribo Profile Nuclease to generate ribosome-protected fragments and isolated via size-exclusion with an Illustra MicroSpin S-400 HR column (GE healthcare). Ribosomal RNA was depleted from both ribosome-protected fragments and RNA-seq libraries using the Illumina Ribo-Zero Gold Kit (Human/Mouse/Rat ...
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bioRxiv - Neuroscience 2022Quote: ... half-filled with deionized water and lined with filter paper (GE Healthcare, WHA1001055) were introduced to the cage as continuously available egg-laying substrates between 3 and 6 days after blood-feeding ...
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bioRxiv - Bioengineering 2023Quote: ... we achieve this using water bottles and the Braino MRS phantom (GE Healthcare) placed in the scanner.
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bioRxiv - Molecular Biology 2019Quote: ... cell lysates corresponding to 2×105 cells in the case of in vivo experiments or 4×105 per sample in the case of nuclease-treated lysates were analyzed by electrophoresis on 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science). The proteins of interest were probed with antibodies against the following proteins ...
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A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activitybioRxiv - Biochemistry 2019Quote: DNA substrates were prepared by diluting plasmid DNA in HyClone water™ (GE healthcare), and unmodified HPLC-purified oligonucleotide substrates (Eurofins ...
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bioRxiv - Microbiology 2023Quote: ... Lake water was firstly filtered through a filter paper (Whatman, GE Healthcare, NY, USA) to remove large particles before filtering through 0.22 μm PES (Polyethersulfone ...
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bioRxiv - Microbiology 2024Quote: Cell-free supernatants were separated by Superdex 30 (GE Healthcare) size exclusion column equilibrated with distilled water and separated at 0.25 ml min-1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Gels were rinsed with water and scanned by Typhoon 9210 Imager (GE Healthcare, Amersham, UK) using the green laser (532 nm) ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified from free nucleotides through G25 spin columns (GE Healthcare). Binding was carried out by incubating 0.5 ng of labelled DNA with 1 μg of unmodified or PARP1 covalently PARylated TRF1 (as above described ...
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bioRxiv - Biochemistry 2019Quote: ... 0.5 mM PMSF and complete EDTA-free protease inhibitors (GE Healthcare). The S100 fraction of lysate supernatant was passed through PD10 column (GE Healthcare ...
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bioRxiv - Systems Biology 2019Quote: ... Free nucleotide was removed using G-25 MicroSpin columns (GE Healthcare). Oligonucleotides were subsequently heated to 75°C and slowly cooled to room temperature to allow proper annealing ...
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bioRxiv - Cell Biology 2019Quote: ... The free dye was removed using a NAP column (GE Healthcare). The concentration of dye and protein were determined by measuring absorbance at 488 and 280 nm ...
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bioRxiv - Biochemistry 2020Quote: ... Free dye was removed using a PD-MidiTrap column (GE Healthcare) and Amicon Ultra-15 Centrifugal Filter Units in a buffer containing 20mM HEPES pH 8.5 ...
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bioRxiv - Cell Biology 2020Quote: ... T151-cells were cultured with tetracycline-free FBS (GE Healthcare, SH30070.03T)22 ...
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bioRxiv - Microbiology 2021Quote: ... free dye was removed using a PD 25 MiniTrapTM (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Immunology 2020Quote: ... Free-unlabeled dye was removed by PD-10 columns (GE healthcare) and concentrated using Amicon® Ultra Centrifugal Filters (Millipore) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... RNA was purified using RNAse-free SPRI beads37,38 (GE Healthcare #65152105050250) and followed by cDNA synthesis using Superscript IV cDNA synthesis kit (Invitrogen #18091050 ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Cell Biology 2019Quote: ... and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare). Purified CiEB1-mCherry in distilled water was microinjected into the dechorionated Ciona eggs together with AF488 phalloidin solution ...
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bioRxiv - Biochemistry 2020Quote: ... free calcein was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the dialysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... free dye was removed through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer (5 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... The free dNTPs were removed on a Sephadex G-25 column (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... pre-equilibrated in endotoxin-free PBS using an AKTA chromatography system (GE Healthcare). Fractions with the protein of interest were pooled and the protein was concentrated and stored at – 80 °C until its use ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins with free cysteines were the purified using thiopropyl sepharose beads (GE Healthcare). Palmitoylation of substrates was determined by quantifying the amount capture relative to unfractionated lysates.
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bioRxiv - Biochemistry 2020Quote: ... Free dye was separated from labelled protein by Illustra NICK columns (GE Healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2019Quote: ... Free dyes were subsequently removed by a Sephadex G-25 column (GE Healthcare). Nucleosomes were assembled by salt gradient dialysis as previously described (Lee and Narlikar ...
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bioRxiv - Immunology 2020Quote: ... Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... Free 89Zr metal was separated using a size exclusion chromatography column (GE Healthcare) and 89Zr-DFO-3N39v4-Fc ...
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bioRxiv - Biophysics 2023Quote: ... free dye was removed using a spin column (PD 25 MiniTrap, GE Healthcare) preequilibrated with measurement buffer (40 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Free Flag peptides were removed using a PD-10 Desalting Column (GE Healthcare). The conjugate was further purified by a new PD-10 Desalting Column ...
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bioRxiv - Biochemistry 2022Quote: ... Free nucleotides were removed from the RNA using Sephadex G25 resin (GE Healthcare) and RNA integrity and purity was verified by denaturing urea-polyacrylamide gel electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... Gels were washed in distilled water and imaged using an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA).