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Citations for GE Life Sciences :
1 - 50 of 109 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Gels were run at a constant 65 W using Sequi-Gen GT Systems from Bio-Rad or vertical electrophoresis systems from CBS Scientific and visualized using an Amersham™ Typhoon™ Biomolecular Imager (GE Healthcare). The intensity of each band was quantified using the ImageQuant software (GE Healthcare)/Image Gauge (Fuji ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Biochemistry 2021Quote: ... The second-dimension separation was carried on a 12.5% SDS-PAGE in a SE 600 Ruby Standard Dual Cooled Vertical Unit (GE Healthcare) at 20 °C ...
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bioRxiv - Genetics 2019Quote: ... centrifuged at 16,000 × g for 30 min and subjected to SDS-PAGE on a SE 250 mini-vertical unit (GE Healthcare). Proteins were transferred to Immobilon-P PVDF membrane (Millipore Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Physiology 2020Quote: ... and a Multiphor II Electrophoresis System (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... separated by electrophoresis and transferred onto nitrocellulose membranes (GE Healthcare). Membranes were blocked at room temperature with either 5% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare) in three running phase (phase 1 ...
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bioRxiv - Microbiology 2019Quote: ... Second dimension separation was performed using an EttanDalt Six Electrophoresis Unit (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... separated by electrophoresis and transferred to a nylon membrane Hybond N+ (GE Healthcare). The probes for the upstream regions of the pks1 (oligonucleotides 9 and 10 ...
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bioRxiv - Microbiology 2020Quote: ... 1D gel electrophoresis analysis was performed with Ettan IPGphor II control software (GE Healthcare). For 2D gel electrophoresis ...
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bioRxiv - Cancer Biology 2022Quote: ... resolved by electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare cat. number 10600003) as described [84] ...
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bioRxiv - Biochemistry 2024Quote: ... The second dimension run was performed in an EttanDalt Six Electrophoresis Unit (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... and resolved by denaturing polyacrylamide electrophoresis before electrophoretic transfer to Nytran Plus membranes (GE Healthcare) and hybridization with [32P]-end labeled oligonucleotide probes at 42°C or direct staining ...
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bioRxiv - Cancer Biology 2020Quote: ... subjected to polyacrylamide gel electrophoresis (PAGE) and electro-transferred onto nitrocellulose membranes (GE Healthcare, Germany). Blocking with 5% non-fat dry milk or bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used 1% agarose gel electrophoresis and a NanoVue spectrophotometer (GE Healthcare Life Sciences, Buckinghamshire, UK). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... After electrophoresis the proteins were transferred to Nitrocellulose membranes (Amersham Protran Premium Nitrocellulose, GE Healthcare, #10600048) via wet transfer at a current of 45V for approximately 20 hours at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... samples were characterized using agarose gel electrophoresis and purified using GFX DNA purification kit (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... 12.5% acrylamide:bis-acrylamide 19:1) electrophoresis and heat/vacuum dried for autoradiography (Amersham Hyperfilm ECL, GE Healthcare). Images were analyzed using ImageJ ...
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bioRxiv - Molecular Biology 2020Quote: ... and resolved by denaturing polyacrylamide electrophoresis before electrophoretic transfer to nylon membranes (GE Healthcare Amersham Hybond-N+) and hybridization with [32P]-end labelled oligonucleotide probes at 42°C ...
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bioRxiv - Biochemistry 2021Quote: ... IEF was then performed on a Multiphor II Electrophoresis System with ImmobilineDryStrip Kit (Amersham Biosciences, Ge Healthcare), in 4 consecutive steps ...
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bioRxiv - Biochemistry 2022Quote: ... Protein purity was assessed by SDS-polyacrylamide gel electrophoresis (PhastGel Homogenous 20 pre-cast gels, GE Healthcare) and the enzyme concentration ([E] ...
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bioRxiv - Pathology 2022Quote: ... followed by electrophoresis and Western blotting on to PVDF membranes (Hybond P, GE Healthcare Bio Sciences, Pittsburgh, Pennsylvania). Primary antibodies against proteins of interest were compared to GAPDH as a housekeeping gene (see Supplemental Table 3) ...
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bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... purified after agarose gel electrophoresis using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare) and ligated into the linearized pHT315-gfp vector ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose Amersham Protran membrane (GE Healthcare). Membranes were blocked at room temperature with either 5% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... separated by gel electrophoresis in 0.7% (w/v) agarose gels and blotted onto Hybond N nylon membranes (GE Healthcare). A ~500 bp probe was produced by PCR amplification (primer pair P19/P20 ...
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bioRxiv - Cancer Biology 2022Quote: ... 25µg of protein were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Protran Premium NC 0.45µm membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1h at RT and incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-antibody complexes were separated by 4–12% Bis-Tris gel electrophoresis at 180 V for 50 min and transferred to nitrocellulose membranes (Protran BA85 0.45µm, GE Healthcare) at 30 V for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were resolved by 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (#10600021, GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... 15μg of protein were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Protran Premium NC 0.45μm membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1h at room temperature (RT ...
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bioRxiv - Microbiology 2022Quote: ... and purified after agarose gel electrophoresis using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare). Insert fragments were amplified with Q5 DNA polymerase (NEB ...
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bioRxiv - Physiology 2019Quote: ... Purity of the isoHb fractions was verified by means of native polyacrylamide gel electrophoresis (PAGE) using a PhastSystem (GE Healthcare) in 10-15% gradient precast gels ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA (1 – 10 μg) was separated using formaldehyde (2.2 M) gel electrophoresis and transferred to a Hybond-NX Nylon membrane (GE Healthcare) via capillary transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Bio-Rad (4–15%) gradient gels were used for gel electrophoresis Proteins were blotted onto 0.2 µm nitrocellulose membranes (Amersham Protran, GE Healthcare) using the Thermo Scientific Pierce G2 Fast Blotter ...
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bioRxiv - Genetics 2019Quote: ... PCR amplicons from each species presenting positive signals for Micropia were separated by 1.5% agarose gel electrophoresis and purified using Illustra GFXTM PCR DNA and Gel Band Purification kit (GE Healthcare) according to the supplier’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... Electrophoresis was performed at 100 V and the gel was imaged using a Typhoon FLA 7000 (GE Healthcare Life Sciences) and quantification was performed using Fiji (63).
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bioRxiv - Molecular Biology 2023Quote: ... The resulting mixtures were analyzed by 6% native TBE polyacrylamide gel electrophoresis and visualized using Typhoon Trio equipment (GE Healthcare).
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bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
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bioRxiv - Microbiology 2023Quote: ... After electrophoresis the gels were stained with ethidium bromide and DNA bands were visualized using a AI600 imager (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... were resolved by SDS–polyacrylamide gel electrophoresis using a 10% acrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (GE Healthcare). After a blocking step (5% non-fat milk in TBS O/N at 4°C) ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences) using a wet transfer method ...
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...