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Citations for GE Life Sciences :
1 - 50 of 2694 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... Insulin Receptor siRNA was obtained by GE Healthcare Dharmacon ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... pH 7.4 (cytokines using Superdex 75 and receptors using Superdex200, prep grade media, GE Healthcare). In addition ...
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bioRxiv - Cell Biology 2020Quote: Receptor was further purified using anion-exchanged chromatography using HiTrap Q HP column (GE Healthcare). Protein was diluted 1:10 with buffer A (20 mM MOPS pH=7.0 ...
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bioRxiv - Biophysics 2023Quote: ... The eluted receptor was desalted from imidazole using a size-exclusion PD10 column (GE Healthcare) equilibrated with the desalt buffer (25 mM HEPES pH=7.5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Next the receptor was purified by size exclusion chromatography on a Sephadex S200 column (GE Healthcare) in 20 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... and the phosphorylation levels of receptor tyrosine kinase were detected with the image analyzer 680 (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Antibodies were then purified using size exclusion columns (Illustra NAP-5 columns, #17085302, GE Healthcare). The absorption of IgG at 280 nm and Cy3B dye at 559 nm of the reaction products was measured by spectrophotometry (NanoDrop ND-1000 Spectrophotometer) ...
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bioRxiv - Immunology 2024Quote: Affinity of bNAbs for Fcγ Receptors was assessed by SPR experiments performed on a Biacore T200 (GE Healthcare). SPR experiments were performed on a T200 apparatus at 25°C in PBS containing 0,05 % P20 surfactant (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... The strips were then washed three times with 5 mL of TBST-BSA and incubated with anti-GST horseradish peroxidase–conjugated antibody (GE Healthcare, RRID:AB_771429) diluted in TBST-BSA ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Immunology 2019Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...